Rabbit anti-Human, Mouse ENSA Polyclonal Antibody | anti-ENSA antibody

ENSA, ID (ENSA, Alpha-endosulfine, ARPP-19e) (Biotin)

Cat. Num. AAA6295734

• Gene Names: ENSA; ARPP-19e
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ENSA; Polyclonal Antibody; ID (ENSA; Alpha-endosulfine; ARPP-19e) (Biotin); ARPP-19e; anti-ENSA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ENSA antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ENSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the Central region of human ENSA.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ENSA Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).)

Western Blot (WB)

(ENSA Antibody (Center) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ENSA antibody detected the ENSA protein (arrow).)

Flow Cytometry (FC/FACS)

(ENSA Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ENSA antibody

ENSA belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes.

Product Categories/Family for anti-ENSA antibody

Antibodies; Proteins

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Gabrielsson, B.G., et al. Mol. Cell. Biochem. 258 (1-2), 65-71 (2004): Thameem, F., et al. Mol. Genet. Metab. 81(1):16-21(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 46389554
• NCBI GeneID: 2029
• NCBI Accession #: NP_996927.1; NP_996929.1
• NCBI GenBank Nucleotide #: NM_207044.1
• UniProt Accession #: O43768; Q5T5H2; Q68D48; Q6FHW0; Q6IAM4; Q6NUL2; Q6VUC6; Q6VUC7; Q6VUC8; Q6VUC9; A8K1Z9; E9PB69
• Molecular Weight: 14,868 Da
• NCBI Official Full Name: alpha-endosulfine isoform 4
• NCBI Official Synonym Full Names: endosulfine alpha
• NCBI Official Symbol: ENSA
• NCBI Official Synonym Symbols: ARPP-19e
• NCBI Protein Information: alpha-endosulfine
• UniProt Protein Name: Alpha-endosulfine
• Protein Family: Alpha-endosulfine
• UniProt Gene Name: ENSA
• UniProt Entry Name: ENSA_HUMAN

NCBI Description

The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

ENSA: a cytoplasmic, highly conserved cAMP-regulated phosphoprotein (ARPP) and regulator of ATP-sensitive potassium (KATP) channels. An endogenous ligand for the sulfonylurea receptor, ABCC8, the regulatory subunit of the KATP channel located on the plasma membrane of pancreatic beta cells. Modulates insulin secretion through the interaction with ABCC8, and may be associated with type 2 diabetes. Eight alternatively spliced isoforms have been observed.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: phosphatase inhibitor activity; protein phosphatase 2A binding; protein phosphatase type 2A regulator activity; protein phosphatase inhibitor activity; potassium channel inhibitor activity; ion channel inhibitor activity; receptor binding

Biological Process: mitosis; regulation of catalytic activity; transport; cell division; negative regulation of catalytic activity; response to glucose stimulus; mitotic cell cycle; G2/M transition of mitotic cell cycle; regulation of insulin secretion; response to nutrient