Mouse Pituitary-specific positive transcription factor 1 (POU1F1) ELISA Kit | POU1F1 elisa kit

Mouse Pituitary-specific positive transcription factor 1 (POU1F1) ELISA Kit

Cat. Num. AAA7207807

• Gene Names: POU1F1; PIT1; CPHD1; GHF-1; Pit-1; POU1F1a
• Reactivity: Mouse
• Synonyms: Pituitary-specific positive transcription factor 1 (POU1F1); Mouse Pituitary-specific positive transcription factor 1 (POU1F1) ELISA Kit; POU1F1 elisa kit

• Reactivity: Mouse

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for POU1F1 elisa kit

Intended Uses: This POSTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine POSTN. This ELISA kit for research use only!

Principle of the Assay: POSTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-POSTN antibody and an POSTN-HRP conjugate. The assay sample and buffer are incubated together with POSTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POSTN concentration since POSTN from samples and POSTN-HRP conjugate compete for the anti-POSTN antibody binding site. Since the number of sites is limited, as more sites are occupied by POSTN from the sample, fewer sites are left to bind POSTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POSTN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for POU1F1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4505955
• NCBI GeneID: 5449
• NCBI Accession #: NP_000297.1
• NCBI GenBank Nucleotide #: NM_000306.3
• Molecular Weight: 35,669 Da
• NCBI Official Full Name: pituitary-specific positive transcription factor 1 isoform alpha
• NCBI Official Synonym Full Names: POU class 1 homeobox 1
• NCBI Official Symbol: POU1F1
• NCBI Official Synonym Symbols: PIT1; CPHD1; GHF-1; Pit-1; POU1F1a
• NCBI Protein Information: pituitary-specific positive transcription factor 1; growth hormone factor 1; POU domain, class 1, transcription factor 1
• UniProt Protein Name: Pituitary-specific positive transcription factor 1
• Protein Family: Pituitary-specific positive transcription factor
• UniProt Gene Name: POU1F1
• UniProt Synonym Gene Names: GHF1; PIT1; PIT-1; GHF-1
• UniProt Entry Name: PIT1_HUMAN

NCBI Description

This gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combined pituitary hormone deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GHF-1: Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'- TAAAT-3'. Belongs to the POU transcription factor family. Class- 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3p11

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: chromatin binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; nuclear transport; regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; cell fate specification; positive regulation of inositol trisphosphate biosynthetic process; positive regulation of multicellular organism growth; negative regulation of transcription from RNA polymerase II promoter; determination of adult life span; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; B cell differentiation; positive regulation of cell proliferation; somatotropin secreting cell development; positive regulation of transcription from RNA polymerase II promoter

Disease: Pituitary Hormone Deficiency, Combined, 1