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833 results for " Anti Human Heat Shock Proteins" - showing 1-50


Western Blot (WB) (The recombinant proteins and tissue lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.A : Recombinant human Crystallin alpha A proteinB : Recombinant human Crystallin alpha B proteinC : Mouse eye tissue lysate)

A crystallin A, Monoclonal Antibody (Cat# AAA200007)

Full Name
A crystallin A antibody
Gene Names
CRYAA; CRYA1; HSPB4; CTRCT9
Reactivity
Human
Applications
EIA, WB
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB) (The Recombinant Human HSP90 (50ng) and Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection systemLane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate)

Hsp90, Monoclonal Antibody (Cat# AAA200047)

Full Name
Hsp90 antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human,
Applications
EIA, Cell lysate, IHC
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL

Alpha B Crystallin, Polyclonal Antibody (Cat# AAA194398)

Full Name
Anti-Alpha B Crystallin Polyclonal Antibody
Gene Names
CRYAB; CRYA2; CTPP2; HSPB5
Applications
Immunoblot
Pricing
$375/0.1 mL | $1,630/5x0.1 mL
Western Blot (WB) (HSPA4 polyclonal antibody. Western Blot analysis of HSPA4 expression in human liver.)

HSPA4, Polyclonal Antibody (Cat# AAA6001642)

Full Name
HSPA4 (Heat Shock 70kD Protein 4, Heat Shock 70-related Protein APG-2, HSP70RY, APG2)
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$620/0.05 mg | $2,775/5x0.05 mg

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA190040)

Full Name
Anti-Hsp70/Hsc70 Monoclonal Antibody
Applications
Immunoblot, Immunohistochemistry
Pricing
$320/0.1 mg | $1,390/5x0.1 mg
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA212109)

Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Immunofluorescence (IF) (Fluorescent image of A549 cell stained with PCM-1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)

PCM-1, Polyclonal Antibody (Cat# AAA9209983)

Full Name
PCM-1 Antibody (Center)
Gene Names
PCM1; PTC4; RET/PCM-1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA210328)

Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 . Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

p23, Monoclonal Antibody (Cat# AAA8001908)

Full Name
p23 Antibody
Gene Names
PTGES3; P23; TEBP; cPGES
Reactivity
Human, Mouse, Rabbit, Chicken, Guinea Pig, Yeast.
Applications
WB, IHC, ICC, IF, IP, EIA, AM
Purity
Protein G Purified
Pricing
$235/0.025 mg | $845/5x0.025 mg
Western Blot (WB) (Western blot analysis of extracts from 3T3, A549, C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.)

BiP/GRP78, Monoclonal Antibody (Cat# AAA475249)

Full Name
Anti-BiP/GRP78 Mouse mAb
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Mouse, Rat
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

HSP60 (Heat Shock Protein 60), Monoclonal Antibody (Cat# AAA4380986)

Full Name
HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (9G10.F8.2).)

GRP94 / HSP90B1, Monoclonal Antibody (Cat# AAA439463)

Full Name
GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Rat Monoclonal Antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human, Monkey, Horse, Cow, Sheep, Pig, Rabbit, Dog, Rat, Mouse, Hamster, Guinea Pig, Chicken, Xenopus laevis
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Western Blot (WB) (Western blot detection of BiP/GRP78 (C-terminus) in Hela, C6, Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.)

BiP/GRP78, Monoclonal Antibody (Cat# AAA475111)

Full Name
Anti-BiP/GRP78 (C-term) Mouse mAb
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Rat
Applications
WB, ICC, IHC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

HSP90, Monoclonal Antibody (Cat# AAA8001863)

Full Name
HSP90 Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Dog, Fish, Shark, Hamster
Applications
WB, IHC, ICC, IF, IP, EIA, AM
Purity
Protein G Purified
Pricing
$440/0.2 mg | $1,760/5x0.2 mg
Western Blot (WB) (Western blot analysis of lysates from H-4-II-E, mouse NIH/3T3 cell line (from left to right), using HSPA8 Antibody (C-term). MBS9208423 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

HSPA8, Polyclonal Antibody (Cat# AAA9208423)

Full Name
HSPA8 Antibody (C-term)
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Mouse, Rat)
Applications
IF, EIA, IHC, FC/FACS, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Breast stained with Crystallin Alpha B Mouse Monoclonal Antibody (CPTC-CYRAB-1).)

Crystallin Alpha B, Monoclonal Antibody (Cat# AAA4380537)

Full Name
Crystallin Alpha B
Gene Names
CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Reactivity
Human. Others not tested.
Applications
WB, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

Hsp70, Monoclonal Antibody (Cat# AAA190039)

Full Name
Anti-Hsp70 Monoclonal Antibody
Applications
ELISA, Flow Cytometry, Immunoblot, Immunohistochemistry, Immunoprecipitation
Pricing
$320/0.1 mg | $1,390/5x0.1 mg
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA214958)

Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant HSPA1A protein (Right) or empty vector (Left) detected with HSPA1A antibody)

HSPA1A, Monoclonal Antibody (Cat# AAA533700)

Full Name
HSPA1A antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Applications
FC/FACS, IF, IHC, WB
Purity
HSPA1A antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-GRP78 Polyclonal Antibody . Tissue: Hippocampal Section. Species: Mouse. Fixation: 4% Formaldehyde for 12 hours at RT. Paraffin embedded. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Rabbit at 1:250 for 1 hour at RT. Counterstain: Hoechst at 1:1000 for 10 min at RT. Localization: Grp78 staining in mouse pyramidal cell layer. Magnification: 20x. Slice thickness: 7 um. Courtesy of: Rachel Reith, NIH/NIMH.)

GRP78, Polyclonal Antibody (Cat# AAA8001985)

Full Name
GRP78 Antibody
Gene Names
Hspa5; BIP; GRP78; GRP 78
Reactivity
Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus, Dog
Applications
WB, IHC, ICC, IF, IP
Purity
Peptide Affinity Purified
Pricing
$275/0.05 mL | $990/5x0.05 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA210664)

Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunohistochemistry (IHC) (Anti-HSP90B1 / GRP94 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

HSP90B1 / GP96 / GRP94, Monoclonal Antibody (Cat# AAA245355)

Full Name
Mouse Monoclonal [clone 2H3] (IgG2a,k) to Human HSP90B1 / GP96 / GRP94
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human
Applications
IHC, IHC - Paraffin, IF, WB
Purity
Protein G Purified
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Western Blot (WB) (Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.)

Hsp90, Monoclonal Antibody (Cat# AAA475175)

Full Name
Anti-Hsp90 Mouse mAb
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Anti- HMOX1 antibody, MBS177453, Western blottingAll lanes: Anti HMOX1 (MBS177453) at 0.5ug/mlWB: Recombinant Human HMOX1 Protein 0.5ngPredicted bind size: 37KDObserved bind size: 37KD )

Heme oxygenase 1, Polyclonal Antibody (Cat# AAA177453)

Full Name
Anti-HMOX1 Antibody
Gene Names
HMOX1; HO-1; HSP32; HMOX1D; bK286B10
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
WB, IHC-P
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

Hsp90, Monoclonal Antibody (Cat# AAA801445)

Full Name
Hsp90 Antibody: Biotin
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat, Rabbit, Chicken, Dog, Fish, Shark, Hamster
Applications
WB, IHC, ICC, IF, EIA, AM
Pricing
$535/0.2 mg | $2,165/5x0.2 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Liver stained withHSP60 Mouse Recombinant Monoclonal Antibody (rGROEL/780).)

HSP60 (Heat Shock Protein 60), Monoclonal Antibody (Cat# AAA4380026)

Full Name
HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey, Chicken, Xenopus laevis, Drosophila.
Does not react with Bacteria, Helminths, Spinach. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

HSP70, Polyclonal Antibody (Cat# AAA8001980)

Full Name
HSP70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Beluga, Bovine, Dog, Fish, Carp, Guinea Pig, Hamster, Monkey, Pig, Sheep, Coral, Plant, Tomato, Tobacco, Shark, Spiny Dogfish Shark, Atlantic Hagfish
Applications
WB, IHC, ICC, IF, IP, EIA
Purity
Peptide Affinity Purified
Pricing
$275/0.025 mL | $1,000/5x0.025 mL
Activity Data (Figure. The binding activity of CTSD with HSP90b1.Cathepsin D (CSTD) is an aspartic protease that depends critically on protonation of its active site Asp residue. Along with Asp-protonation, lower pH also leads to conformational switch in cathepsin-D: the N-terminal segment of the protease moves out of the active site as pH drops. Similar to other aspartic protainases, cathepsin D accommodates up to 8 amino acid residues in the binding cleft of the active site. The main physiological functions of cathepsin D consist of metabolic degradation of intracellular proteins, activation and degradation of polypeptide hormones and growth factors, activation of enzymatic precursors, processing of enzyme activators and inhibitors, brain antigen processing and regulation of programmed cell death. Besides, Heat Shock Protein 90kDa Beta 1 (HSP90b1) has been identified as an interactor of CSTD, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CSTD and recombinant human HSP90b1. Briefly, CSTD were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to HSP90b1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CSTD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CSTD and HSP90b1 was shown in Figure 1, and this effect was in a dose dependent manner.)

Cathepsin D (CTSD), Active Protein (Cat# AAA2105134)

Full Name
Active Cathepsin D (CTSD)
Gene Names
CTSD; CPSD; CLN10; HEL-S-130P
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$245/0.01 mg | $530/0.05 mg | $820/0.1 mg | $1,015/0.2 mg | $1,985/0.5 mg | $2,935/1 mg | $7,180/5 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HSPA5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

HSPA5, Polyclonal Antibody (Cat# AAA125484)

Full Name
HSPA5 Polyclonal Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Cell lysates of HeLa (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp60 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

HSP60, Monoclonal Antibody (Cat# AAA200078)

Full Name
HSP60 antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human
Applications
EIA, WB, IHC, ICC, IF, FC/FACS
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Testing Data (Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)

V5-TAG, Monoclonal Antibody (Cat# AAA216888)

Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S82 antibody at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1min.)

HSPB1-S82, Antibody (Cat# AAA125215)

Full Name
Phospho-HSPB1-S82 Polyclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 3A3 . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 40X.)

HSP70, Monoclonal Antibody (Cat# AAA8001917)

Full Name
HSP70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat, Amphibians, Chicken, Fish, Yeast, Fruit Fly, Bacteria, Brine Shrimp
Applications
WB, IHC, ICC, IF, IP, AM
Purity
Protein G Purified
Pricing
$265/0.025 mg | $980/5x0.025 mg
Western Blot (WB) (Anti-Hsp70 antibody, Western blottingAll lanes: Anti Hsp70 at 0.5ug/ml Lane 1: 293T Whole Cell Lysate at 40ug Lane 2: HELA Whole Cell Lysate at 40ug Lane 3: A549 Whole Cell Lysate at 40ug Lane 4: A431 Whole Cell Lysate at 40ug Lane 5: PANC Whole Cell Lysate at 40ug Predicted bind size: 70KD Observed bind size: 70KD)

Hsp70, Polyclonal Antibody (Cat# AAA176327)

Full Name
Anti-Hsp70 antibody
Gene Names
HSPA1B; HSP70-2; HSP70-1B
Reactivity
Human, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Chicken Anti-Hsp70 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Chicken Anti-Hsp70 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Chicken (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

HSP70, Polyclonal Antibody (Cat# AAA8002016)

Full Name
HSP70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, EIA
Purity
PEG Purified
Pricing
$275/0.025 mg | $1,000/5x0.025 mg
SDS-Page (SDS-PAGE Analysis Purified ER-beta Mouse Monoclonal Antibody (ESR2/3207). Confirmation of Purity and Integrity of Antibody.)

ER-beta-1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA4380884)

Full Name
ER-beta-1 (Estrogen Receptor beta-1)
Gene Names
ESR2; Erb; ESRB; ODG8; ESTRB; NR3A2; ER-BETA; ESR-BETA
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Erp57 (Grp58) Monoclonal Antibody, Clone Map.ERP57 . Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Erp57 (Grp58) Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erp57 (Grp58) Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

ERp57, Monoclonal Antibody (Cat# AAA8001922)

Full Name
ERp57 (GRP58) Antibody
Gene Names
PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea Pig, Hamster, Monkey, Pig, Rabbit
Applications
WB, IHC, ICC, IF, IP
Purity
Protein G Purified
Pricing
$280/0.05 mg | $1,015/5x0.05 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HSPA9 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

HSPA9, Polyclonal Antibody (Cat# AAA125123)

Full Name
HSPA9 Polyclonal Antibody
Gene Names
HSPA9; CSA; MOT; MOT2; CRP40; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot detection of Hsp60 in Hela, Raji, Jurkat, C6, C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.)

Hsp60, Monoclonal Antibody (Cat# AAA475226)

Full Name
Anti-Hsp60 Mouse mAb
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat
Applications
WB, IF, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot detection of Hsp90 beta in Hela, 3T3, C6, CHO-K1 and COS7 cell lysates using Hsp90 beta mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.)

Hsp90 beta, Monoclonal Antibody (Cat# AAA475214)

Full Name
Anti-Hsp90 beta Mouse mAb
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 . Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

GRP94, Monoclonal Antibody (Cat# AAA8001858)

Full Name
GRP94 Antibody
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Hamster, Horse, Monkey, Pig, Rabbit, Sheep, African Clawed Frog
Applications
WB, ICC, IF, IP, FC/FACS
Purity
Protein G Purified
Pricing
$245/0.05 mg | $890/5x0.05 mg
Testing Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP27 Mouse Monoclonal Antibody (CPTC-HSPB1-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Heat Shock 27kDa Protein 1, Monoclonal Antibody (Cat# AAA4380593)

Full Name
Heat Shock 27kDa Protein 1
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human. Others not tested.
Applications
WB, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.)

Hsp90, Monoclonal Antibody (Cat# AAA800582)

Full Name
Hsp90 Antibody
Reactivity
Bovine, Chicken, Human, Mouse, Porcine (Pig), Rat, Rabbit
Applications
IP, IHC, WB, EIA, AM
Purity
Protein G Purified
Pricing
$275/0.025 mg
Western Blot (WB) (Western blot detection of Hsp70 (C-terminus) in Hela and HsHela cell lysates using Hsp70 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 70KDa.Observed band size: 70KDa.)

Hsp70, Monoclonal Antibody (Cat# AAA475008)

Full Name
Anti-Hsp70 (C-term) Mouse mAb
Gene Names
HSPA1B; HSP70-2; HSP70.2; HSP70-1B
Reactivity
Human
Applications
WB, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using DNAJB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

DNAJB1, Polyclonal Antibody (Cat# AAA9125485)

Full Name
DNAJB1 Polyclonal Antibody
Gene Names
DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)

HSP70/HSC70, Monoclonal Antibody (Cat# AAA8001860)

Full Name
HSP70/HSC70 Antibody
Gene Names
HSPA2; HSP70
Reactivity
Human, Mouse, Rat, Bovine, Sheep, Dog, Beluga, Fish, Guinea Pig, Pig, Hamster, Rabbit, Chicken, African Clawed Frog, Fruit Fly, Yeast
Applications
WB, ICC, IF, IHC, IP
Purity
Protein G Purified
Pricing
$275/0.05 mg | $990/5x0.05 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at-20 degree C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.)

HSP60, Monoclonal Antibody (Cat# AAA8001868)

Full Name
HSP60 Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Bovine, Dog, Chicken, Fruit Fly, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, African Clawed Frog, Plant, Soybean, Silkworm
Applications
WB, IHC, IP, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$355/0.2 mg | $1,360/5x0.2 mg
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using Phospho-HSPB1-S15 antibody at 1:1000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5min.)

HSPB1-S15, Antibody (Cat# AAA125428)

Full Name
Phospho-HSPB1-S15 Polyclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at-20 degree C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.)

HSP60, Monoclonal Antibody (Cat# AAA8001867)

Full Name
HSP60 Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Bovine, Dog, Chicken, Fruit Fly, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Sheep, African Clawed Frog, Plant, Soybean, Silkworm
Applications
WB, IHC, IP, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$240/0.05 mg | $860/5x0.05 mg
Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A431 cell lysate Lane 2.: HepG2 cell lysate )

Hsp27, Monoclonal Antibody (Cat# AAA200046)

Full Name
Hsp27 antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
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