Mouse Hsp70 Monoclonal Antibody
Anti-Hsp70 Monoclonal Antibody
Applications
ELISA, Flow Cytometry, Immunoblot, Western Blot, Immunohistochemistry, Immunoprecipitation
Synonyms
Hsp70; Monoclonal Antibody; Anti-Hsp70 Monoclonal Antibody; anti-Hsp70 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
Hsp70 [Mouse monoclonal antibody to Hsp70]
This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436-503 of human Hsp70.
This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436-503 of human Hsp70.
Form/Format
100ug in PBS, pH 7.4, purified by Protein G affinity chromatography.
Sequence Length
608
Applicable Applications for anti-Hsp70 antibody
ELISA, Flow Cytometry, Immunoblot, Immunohistochemistry, Immunoprecipitation
Application Notes
Immunoblotting: 1ug/ml. A band of 70kDa is detected.
Immunohistochemistry: 5-10ug/ml
Immunoprecipitation: 1-10ug/ml
ELISA: 1-10ug/ml
Flow cytometry: 10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
Immunohistochemistry: 5-10ug/ml
Immunoprecipitation: 1-10ug/ml
ELISA: 1-10ug/ml
Flow cytometry: 10ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
Antigen
Human Hsp70 purified from HeLa cells.
Preservatives
0.1% sodium azide.
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
Related Product Information for anti-Hsp70 antibody
Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat-shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C-terminus binds proteins and polypeptides. Hsp70s bind nascent polypeptides as well as partially folded intermediates of proteins, thus preventing their aggregation and/or misfolding. Binding of ATP triggers a conformational change that results in the release of bound protein. Hsp70s function in protein synthesis, protein folding and oligomerization, and protein transport.
Product Categories/Family for anti-Hsp70 antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
67,016 Da
NCBI Official Full Name
HSP70
UniProt Protein Name
HSP70
Protein Family
UniProt Entry Name
Q40058_HORVU
Uniprot Description
Sequence similarities: Belongs to the heat shock protein 70 family. RuleBase RU003322
Similar Products
Product Notes
The Hsp70 (Catalog #AAA190039) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hsp70 can be used in a range of immunoassay formats including, but not limited to, ELISA, Flow Cytometry, Immunoblot, Immunohistochemistry, Immunoprecipitation. Immunoblotting: 1ug/ml. A band of 70kDa is detected. Immunohistochemistry: 5-10ug/ml Immunoprecipitation: 1-10ug/ml ELISA: 1-10ug/ml Flow cytometry: 10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate. Researchers should empirically determine the suitability of the Hsp70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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