Mouse anti-Human HSP60 (Heat Shock Protein 60) Monoclonal Antibody | anti-HSP60 antibody

HSP60 (Heat Shock Protein 60) (Mitochondrial Marker)

Cat. Num. AAA4380986

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: HSP60 (Heat Shock Protein 60); Monoclonal Antibody; HSP60 (Heat Shock Protein 60) (Mitochondrial Marker); 60kDa chaperonin; 60kDa heat shock protein mitochondrial; Chaperonin; 60-KD (CPN60); GROEL; HLD4; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; Short heat shock protein 60 Hsp60s1; Spastic paraplegia 13 (SPG13); anti-HSP60 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CPTC-HSPD1-1
• Specificity: The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes, and the regulation of protein folding. The mitochondrial and cytosolic localization of HSP60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker for T cell recognition, as well as being involved in a danger signal cascade immune response. HSP60 has been shown to influence apoptosis in tumor cells, and changes in its expression level may serve as a biomarker, as down-regulated HSP60 expression indicates a poor prognosis as well as a risk of tumor infiltration development, especially with regard to urothelial carcinomas. In ovarian cancer, decreased expression of HSP60 correlates with aggressive tumor types, while overexpression is correlated with a better patient prognosis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 573

• Applicable Applications for anti-HSP60 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 1-2ug/0.5 million cells in 0.1ml; IF: 1-2ug/ml; WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HEP-2, PC3 or HeLa cells. Breast or Colon Carcinoma.
• Immunogen: Recombinant full-length human HSPD1 protein
• Cellular Localization: Mitochondria in cytoplasm
• Hu-chromosome Location: 2q33.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

Immunofluorescence (IF)

(Immunofluorescence Analysis of human MCF-7 cells labeling GPI with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells labeling HSP60 with HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1) followed by Goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red))

Western Blot (WB)

(Western Blot Analysis of HEP-2 and PC3 cell lysates using HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1).)

SDS-Page

(SDS-PAGE Analysis Purified HSP60 Mouse Monoclonal Antibody (CPTC-HSPD1-1). Confirmation of Purity and Integrity of Antibody.)

References

Cheng, Ming Y., et al. 29 Nov. 1990.

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NP_002147.2
• UniProt Accession #: P10809
• Molecular Weight: 60kDa
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock protein family D (Hsp60) member 1
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• Protein Family: Hydrogenosomal chaperonin
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN

NCBI Description

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Uniprot Description

HSP60: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Interacts with HRAS. Interacts with HBV protein X and HTLV-1 protein p40tax. Belongs to the chaperonin (HSP60) family.

Protein type: Chaperone; Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: extracellular space; protein complex; cell surface; mitochondrion; early endosome; coated pit; cytosol; secretory granule; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; plasma membrane; lipopolysaccharide receptor complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: protein binding; lipopolysaccharide binding; p53 binding; ubiquitin protein ligase binding; chaperone binding; unfolded protein binding; double-stranded RNA binding; ATPase activity; DNA replication origin binding; ATP binding; single-stranded DNA binding

Biological Process: caspase activation; B cell proliferation; viral reproduction; B cell activation; protein stabilization; T cell activation; positive regulation of interleukin-12 production; positive regulation of apoptosis; protein maturation; isotype switching to IgG isotypes; positive regulation of interleukin-6 production; B cell cytokine production; positive regulation of interleukin-10 production; response to unfolded protein; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; 'de novo' protein folding; protein refolding; positive regulation of T cell mediated immune response to tumor cell; positive regulation of T cell activation; chaperone-mediated protein complex assembly; positive regulation of interferon-alpha production; negative regulation of apoptosis; positive regulation of macrophage activation

Disease: Spastic Paraplegia 13, Autosomal Dominant; Leukodystrophy, Hypomyelinating, 4