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223 results for " 14 3 3 sigma Antibody" - showing 1-50
Western Blot (WB)
(Anti-14-3-3 sigma antibody, MBS175643, Western blottingAll lanes: Anti 14-3-3 sigma (MBS175643) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SE620 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 28KD)
14-3-3 sigma, Polyclonal Antibody (Cat# AAA175643)
Full Name
Anti-14-3-3 sigma antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
((0. 03ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
14-3-3 sigma/Stratifin, Polyclonal Antibody (Cat# AAA421444)
Full Name
Goat anti-14-3-3 sigma/Stratifin Antibody
Gene Names
SFN; YWHAS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western Blot: Sample: Rat Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Human SFN Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Stratifin (SFN), Polyclonal Antibody (Cat# AAA2004708)
Full Name
Polyclonal Antibody to Stratifin (SFN)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Immunohistochemistry (IHC)
(MBS9600058 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
)
BAX, Polyclonal Antibody (Cat# AAA9600058)
Full Name
BAX Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page
(SDS-PAGE Analysis Purified GST Mu3 Mouse Monoclonal Antibody (CPTC-GSTMu3-1). Confirmation of Purity and Integrity of Antibody.)
Glutathione S-Transferase Mu3 (GSTM3), Monoclonal Antibody (Cat# AAA4380581)
Full Name
Glutathione S-Transferase Mu3 (GSTM3)
Gene Names
GSTM3; GST5; GSTB; GTM3; GSTM3-3
Reactivity
Human. Others not tested.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Skin stained with Stratifin Mouse Monoclonal Antibody (CPTC-SFN-2).)
14-3-3 Sigma/Stratifin, Monoclonal Antibody (Cat# AAA4380575)
Full Name
14-3-3 Sigma/Stratifin
Gene Names
SFN; YWHAS
Reactivity
Human. Others not tested.
Applications
WB, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis on HepG2 cell lysate using Bax Antibody.The lane on the left is treated with the antigen-specific peptide.)
BAX, Polyclonal Antibody (Cat# AAA9600035)
Full Name
BAX Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Placenta stained with GSTM1 Mouse Monoclonal Antibody (GSTMu1-3).)
Glutathione S-Transferase Mu1 (GSTM1), Monoclonal Antibody (Cat# AAA4380579)
Full Name
Glutathione S-Transferase Mu1 (GSTM1)
Gene Names
GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
Reactivity
Human. Others not tested.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using SFN Antibody. The lane on the left is treated with the antigen-specific peptide.)
SFN, Polyclonal Antibody (Cat# AAA9606848)
Full Name
SFN Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SFN antibody.)
SFN, Polyclonal Antibody (Cat# AAA9408332)
Full Name
SFN Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL
Western Blot (WB)
(Western blot analysis of cell lysates using Rabbit anti 14-3-3 sigma antibody)
14-3-3 Sigma, Polyclonal Antibody (Cat# AAA225067)
Full Name
Rabbit anti 14-3-3 Sigma
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, WB
Purity
Purified whole serum by affinity chromatography.
Pricing
$430/0.05 mL | $1,780/5x0.05 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SFN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
SFN, Polyclonal Antibody (Cat# AAA128099)
Full Name
SFN Polyclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded section of Human Esophageal squamous cell carcinoma Cancer)
SFN, Polyclonal Antibody (Cat# AAA5313080)
Full Name
SFN Antibody
Gene Names
SFN; YWHAS
Applications
WB, IHC
Purity
Affinity Chromatography
Pricing
$660/0.1 mg | $2,965/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from A431,Hela cell line (from left to right), using SFN Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
SFN, Polyclonal Antibody (Cat# AAA9231736)
Full Name
SFN Antibody (C-term)
Gene Names
SFN; YWHAS
Reactivity
Human
Predicted: Mouse, Bovine
Predicted: Mouse, Bovine
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat liver tissue slide using MBS7607418 (SFN antibody) at dilution of 1:100)
SFN, Antibody (Cat# AAA7607418)
Full Name
Anti-SFN Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
>=95% as determined by SDS-PAGE
Immunogen Affinity Purified
Immunogen Affinity Purified
Pricing
$305/0.1 mg | $1,275/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from Hela cell line,mouse bladder tissue lysate,A431 cell line (from left to right), using SFN Antibody (Center). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
SFN, Polyclonal Antibody (Cat# AAA9231786)
Full Name
SFN Antibody (Center)
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human skin stained with 14.3.3 sigma antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis.)
14.3.3 sigma, Monoclonal Antibody (Cat# AAA531075)
Full Name
14.3.3 sigma antibody (BSA/Azide Free)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
IF, IHC, IP
Purity
14.3.3 Sigma antibody (BSA/Azide Free) was purified by Protein G chromatography.
Pricing
$1,095/0.1 mL | $4,880/5x0.1 mL
14.3.3 sigma, Monoclonal Antibody (Cat# AAA530976)
Full Name
14.3.3 sigma antibody
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
IF, IHC, IP
Purity
14.3.3 Sigma antibody was purified by Protein G chromatography.
Pricing
$1,015/0.5 mL | $1,655/1 mL | $7,385/5x1 mL
Western Blot (WB)
(Expression analysis of 14-3-3 sigma . Anti- 14-3-3 sigma antibody (11-1020) was used at 1 and 2 µg/ml on Molt-4 lysate.)
14-3-3 sigma, Polyclonal Antibody (Cat# AAA668151)
Full Name
14-3-3 sigma Polyclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
WB
Pricing
$305/0.1 mg | $160/0.025 mg | $1,315/5x0.1 mg
Western Blot (WB)
(Western Blot of MBS633309 |Human cells-13)
14-3-3, sigma, Monoclonal Antibody (Cat# AAA633309)
Full Name
14-3-3, sigma (SFN, Stratifin, HGNC:10773) (BSA & Azide Free)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
WB, IP, IHC, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$810/0.1 mg | $3,645/5x0.1 mg
Western Blot (WB)
(WB: EGF-stimulated A431 cell lysate probed with MBS609422 (0.5ug/ml).)
14-3-3, sigma, Monoclonal Antibody (Cat# AAA609422)
Full Name
14-3-3, sigma (SFN, Stratifin, HGNC:10773)
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$745/0.2 mg | $3,355/5x0.2 mg
14-3-3, sigma, Polyclonal Antibody (Cat# AAA628869)
Full Name
14-3-3, sigma (14-3-3 Protein sigma, Stratifin, SFN, Epithelial Cell Marker Protein 1, Er, HGNC:10773, HME1, Mme1, OTTHUMP00000004242, YWHAS)
Gene Names
SFN; YWHAS
Reactivity
Canine, Human, Mouse, Rat
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$790/0.1 mg | $3,555/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of whole cell extracts from different cell lines using antibody MBS632037. Samples were electrophoresced usng Novex NuPAGE 10% Bris-Tris gel. Resolved proteins were transferred onto a nitrocellulose membrane and blocked with 5% skim milk. Detection was by chemiluminescence using IgG (HRP) goat anti-mouse secondary antibody and Novex ECL chemiluminescent reagent kit (Pierce Cat. # WP20005).)
14-3-3, sigma, Monoclonal Antibody (Cat# AAA632037)
Full Name
14-3-3, sigma (SFN, Stratifin, HGNC:10773)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
WB, IP, IHC, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$765/0.5 mL | $3,440/5x0.5 mL
Chemiluminescence
(0.2µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Online Catalogue > 14-3-3 sigma to FZD9 > Goat Anti-CXCL2 / MIP-2 Antibody)
Macrophage inflammatory protein 2-alpha, Polyclonal Antibody (Cat# AAA620038)
Full Name
Macrophage inflammatory protein 2-alpha (CXCL2, Chemokine (C-X-C motif) ligand 2, CINC-2a, GRO2, GROb, GROB, Gro-beta, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha precursor, MGSA-b, MGSA beta, MIP2, MIP2A, MIP-2a, MIP2-alpha, SC
Gene Names
CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Pricing
$620/0.1 mg | $2,775/5x0.1 mg
Western Blot
(Western Blot analysis of 14.3.3 tau antibodyCell lysates of Jurkat (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 tau (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conj ugated to HRP and an ECL detection system.)
14.3.3 tau, Monoclonal Antibody (Cat# AAA533324)
Full Name
14.3.3 tau antibody
Reactivity
Human
Applications
EIA, WB
Purity
14-3-3 tau antibody was purified by protein-G affinity chromatography
Pricing
$435/0.1 mL | $1,945/5x0.1 mL
14-3-3-pan, Blocking Peptide (Cat# AAA8244301)
Full Name
14-3-3-pan (AcK51/49) Blocking Peptide
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, Mouse, Rat, Bovine, Sheep, Zebrafish
Applications
BL
Purity
>85%
Pricing
$220/1 mg | $465/5 mg | $2,050/5x5 mg
Western Blot (WB)
(Western blot analysis of 14-3-3-pan (AcK51/49) expression in mouse spleen (A) whole cell lysates.)
14-3-3-pan, Polyclonal Antibody (Cat# AAA8242883)
Full Name
Anti-14-3-3-pan (AcK51/49) Acetyl Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, Mouse, Rat, Bovine, Sheep, Zebrafish
Applications
WB
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$185/0.03 mL | $275/0.1 mL | $380/0.2 mL | $1,650/5x0.2 mL
stratifin, Polyclonal Antibody (Cat# AAA714324)
Full Name
Rabbit anti-human stratifin polyclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
EIA
Purity
Antigen Affinity Purified
Pricing
$250/0.05 mL | $540/0.15 mL | $2,410/5x0.15 mL
Western Blot (WB)
(Western blot analysis 14-3-3sigma (Phospho-Ser186) using EGF treated HepG2 whole cell lysates)
14-3-3sigma, Polyclonal Antibody (Cat# AAA9416827)
Full Name
14-3-3sigma (Phospho-Ser186) Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse Brain Tissue lysate The Recombinant Protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 epsilon antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 14-3-3 Zeta Recombinant Protein Lane 2.: 14-3-3 Beta Recombinant Protein Lane 3.: 14-3-3 Epsilon Recombinant Protein Lane 4.: 14-3-3 Eta Recombinant Protein Lane 5.: 14-3-3 Gamma Recombinant Protein Lane 6.: 14-3-3 Sigma Recombinant Protein Lane 7.: 14-3-3 Tau Recombinant Protein)
14-3-3 epsilon, Monoclonal Antibody (Cat# AAA200428)
Full Name
14-3-3 epsilon antibody
Gene Names
YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells and RAW264.7 cells, using 14-3-3 sigma antibody.)
14-3-3 sigma, Antibody (Cat# AAA8509803)
Full Name
14-3-3 sigma Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(SFN Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SFN antibody detected the SFN protein (arrow).)
SFN, Polyclonal Antibody (Cat# AAA6004754)
Full Name
SFN, CT (SFN, HME1, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
EL/EIA, WB, IHC, FC/FACS, IF
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
14-3-3 sigma, Polyclonal Antibody (Cat# AAA8520993)
Full Name
14-3-3 sigma Polyclonal Antibody
Gene Names
SFN; YWHAS
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of 14-3-3 sigma expression in A431 cell lysate (MBS179566).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFN )
14-3-3 sigma, Monoclonal Antibody (Cat# AAA179566)
Full Name
Anti-14-3-3 sigma Rabbit Monoclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
((Figure. Western Blot; Sample: Recombinant protein.))
Stratifin (SFN), Polyclonal Antibody (Cat# AAA2001851)
Full Name
Biotin-Linked Antibody to Stratifin (SFN)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$175/0.1 mL | $220/0.2 mL | $375/0.5 mL | $460/1 mL | $1,295/5x1 mL
Immunohistochemistry (IHC)
(Human Skin (formalin-fixed, paraffin-embedded) stained with SFN at 1:250 followed by biotinylated goat rabbit IgG, alkaline phosphatase-streptavidin and chromogen.)
SFN, Polyclonal Antibody (Cat# AAA6007229)
Full Name
SFN, NT (14-3-3 Protein sigma, Epithelial Cell Marker Protein 1, Stratifin, HME1)
Gene Names
SFN; YWHAS
Reactivity
Sheep
Applications
WB, IHC
Purity
Serum
Serum
Serum
Pricing
$800/0.05 mL | $3,585/5x0.05 mL
Immunohistochemistry (IHC)
(Biotinylated (0.1ug/ml) staining of Mouse Skin lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
14-3-3 sigma/Stratifin, Antibody (Cat# AAA423714)
Full Name
Goat anti-14-3-3 sigma/Stratifin, Biotinylated Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse
Expected from sequence similarity: Rat, Dog, Pig
Expected from sequence similarity: Rat, Dog, Pig
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
SFN, Antibody (Cat# AAA8501040)
Full Name
SFN Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
$330/0.1 mL | $570/0.1 mL (Biotin) | $1,425/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(SFN Antibody (Center) western blot analysis in A431 cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the SFN antibody detected the SFN protein (arrow).)
SFN, Polyclonal Antibody (Cat# AAA6010269)
Full Name
SFN, ID (SFN, HME1, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
14-3-3 sigma, Monoclonal Antibody (Cat# AAA8534148)
Full Name
14-3-3 sigma
Gene Names
SFN; YWHAS
Reactivity
Human, Rat
Applications
WB, IHC
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: SFN knockout Hela cell lysate; Predicted MW: 28kd Observed MW: 30kd Primary Ab: 1ug/ml Rabbit Anti-Human SFN Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Stratifin (SFN), Polyclonal Antibody (Cat# AAA2013396)
Full Name
FITC-linked Antibody to Stratifin (SFN)
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
IHC, ICC, IF, WB
Purity
Affinity Chromatography
Pricing
$205/0.1 mL | $255/0.2 mL | $470/0.5 mL | $575/1 mL | $1,615/5x1 mL
Western Blot (WB)
(Western blot analysis of lysates from HT29 cells and RAW264.7 cells, using 14-3-3 sigma Antibody. The lane on the right is blocked with the synthesized peptide.)
14-3-3 sigma, Polyclonal Antibody (Cat# AAA9505587)
Full Name
14-3-3 sigma Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
Western Blot (WB)
(Western blot analysis of 14-3-3 sigma expression in HT29 (A), MCF7 (B) whole cell lysates.)
14-3-3 sigma, Polyclonal Antibody (Cat# AAA8291554)
Full Name
Anti-14-3-3 sigma Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
Purified by immunogen affinity chromatography
Pricing
$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant SFN, Mouse.)
Stratifin, Polyclonal Antibody (Cat# AAA2027492)
Full Name
Polyclonal Antibody to Stratifin (SFN)
Gene Names
Sfn; Er; Mme1; Ywhas
Reactivity
Mouse
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $210/0.2 mL | $355/0.5 mL | $435/1 mL | $1,240/5x1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/25.)
SFN, Polyclonal Antibody (Cat# AAA9416427)
Full Name
SFN Antibody
Gene Names
SFN; YWHAS
Reactivity
Human
Applications
IHC
Purity
Antigen Affinity Purification
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of 14-3-3 sigma using anti-14-3-3 sigma antibody (MBS1750577).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela cell lysate,Lane 2: human placenta tissue lysate,Lane 3: human MCF-7 cell lysate,Lane 4: human 22RV1 cell lysate,Lane 5: rat lung tissue lysate,Lane 6: rat PC-12 cell lysate,Lane 7: mouse lung tissue lysate,Lane 8: mouse HEPA1-6 cell lysate,Lane 9: mouse NIH3T3 cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 sigma antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for 14-3-3 sigma at approximately 28KD. The expected band size for 14-3-3 sigma is at 28KD. )
14-3-3 sigma, Polyclonal Antibody (Cat# AAA1750577)
Full Name
Anti-14-3-3 sigma Picoband antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Bax, Blocking Peptide (Cat# AAA9223528)
Full Name
Bax Antibody (Center) Blocking peptide
Gene Names
BAX; BCL2L4
BAX, Blocking Peptide (Cat# AAA9219752)
Full Name
BAX Antibody (N-term) Blocking Peptide
Gene Names
BAX; BCL2L4
SFN, Blocking Peptide (Cat# AAA9223772)
Full Name
SFN Antibody (Center) Blocking Peptide
Gene Names
SFN; YWHAS
Stratifin (SFN), Polyclonal Antibody (Cat# AAA2096368)
Full Name
Biotin-Linked Polyclonal Antibody to Stratifin (SFN)
Gene Names
SFN; YWHAS
Reactivity
Mouse
Applications
WB, IHC
Pricing
$180/0.1 mL | $220/0.2 mL | $385/0.5 mL | $475/1 mL | $1,320/5x1 mL
