Rabbit 14-3-3 Sigma Polyclonal Antibody | anti-SFN antibody

Rabbit anti 14-3-3 Sigma

Cat. Num. AAA225067

• Gene Names: SFN; YWHAS
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: 14-3-3 Sigma; Polyclonal Antibody; Rabbit anti 14-3-3 Sigma; 14-3-3 Sigma Antibody; anti-SFN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 248

• Applicable Applications for anti-SFN antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IF: 1:50-1:200; IHC-Paraffin: 1:50-1:200; WB: 1:500-1:2000
• Immunogen: Recombinant human 14-3-3 sigma
• Preperation: Antiserum to 14-3-3 sigma was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell lysates using Rabbit anti 14-3-3 sigma antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver injury using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal tissue using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse liver using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using Rabbit anti 14-3-3 sigma antibody at dilution of 1/100)

Related Product Information for anti-SFN antibody

Rabbit anti 14-3-3 sigma antibody recognizes 14-3-3 sigma, also known as SFN, YWHAS or stratifin. 14-3-3 Sigma is a member of the 14-3-3 family of proteins. 14-3-3 proteins regulate multiple cell signaling pathways through their interaction with discrete protein sequences.

NCBI and Uniprot Product Information

• NCBI GI #: 398953
• NCBI GeneID: 2810
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 24,336 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma
• UniProt Protein Name: 14-3-3 protein sigma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1

NCBI Description

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Uniprot Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.