Goat 14-3-3, sigma Polyclonal Antibody | anti-SFN antibody

14-3-3, sigma (14-3-3 Protein sigma, Stratifin, SFN, Epithelial Cell Marker Protein 1, Er, HGNC:10773, HME1, Mme1, OTTHUMP00000004242, YWHAS)

Cat. Num. AAA628869

• Gene Names: SFN; YWHAS
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: 14-3-3; sigma; Polyclonal Antibody; sigma (14-3-3 Protein sigma; Stratifin; SFN; Epithelial Cell Marker Protein 1; Er; HGNC:10773; HME1; Mme1; OTTHUMP00000004242; YWHAS); Anti -14-3-3; anti-SFN antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human 14-3-3 sigma at ~28kD in human skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Species Crossreactivity: Mouse, rat and dog.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, 0.5 mg/ml, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-SFN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:64000; Western Blot: 0.1-0.3ug/ml
• Immunogen: Synthetic peptide of 14-3-3 sigma.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SFN antibody

14-3-3 proteins are 30kD polypeptides with nine closely related members in mammals. They are also found in plants and fungi. 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins which bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.

14-3-3 proteins form homo- and heterodimeric cup-like structures that bind to discrete phosphoserine-containing motifs. In some instances, 14-3-3 proteins appear to export their binding partners from the nucleus to the cytoplasm in a phosphorylation and Crm1-dependent manner.
14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1 and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo. Expression of 14-3-3 sigma is higher in normal epithelial cells.

Product Categories/Family for anti-SFN antibody

Antibodies; Abs to 14-3-3

NCBI and Uniprot Product Information

• NCBI GI #: 5454052
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142.3
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 27,774 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma; 14-3-3 sigma; OTTHUMP00000004242; epithelial cell marker protein 1
• UniProt Protein Name: 14-3-3 protein sigma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1
• UniProt Entry Name: 1433S_HUMAN

Uniprot Description

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; regulation of epidermal cell division; release of cytochrome c from mitochondria; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth