Mouse anti-Human 14.3.3 sigma Monoclonal Antibody | anti-14.3.3 sigma antibody

14.3.3 sigma antibody (BSA/Azide Free)

Cat. Num. AAA531075

• Gene Names: SFN; YWHAS
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: 14.3.3 Sigma antibody (BSA/Azide Free) was purified by Protein G chromatography.
• Synonyms: 14.3.3 sigma; Monoclonal Antibody; 14.3.3 sigma antibody (BSA/Azide Free); Monoclonal 14.3.3 Sigma; Anti-14.3.3 Sigma; 14/3/3 sigma; 14-3-3 sigma; 14-3-3 Sigma; anti-14.3.3 sigma antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1 kappa
• Clone Number: 1433S01
• Specificity: Human
• Purity/Purification: 14.3.3 Sigma antibody (BSA/Azide Free) was purified by Protein G chromatography.
• Form/Format: Antibody purified from ascites fluid by protein G chromotography and supplied in 10mM PBS, pH 7.4.
• Sequence Length: 248

• Applicable Applications for anti-14.3.3 sigma antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: IHC-F: 2-4 ug/ml; IHC-P: 2-4 ug/ml; IP: 2 ug/ml protein lysate
• Biological Significance: 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo.
• Immunogen: 14.3.3 Sigma antibody (BSA/Azide-free) was raised in mouse using recombinant human 14-3-3 sigma protein as the immunogen.
• Preparation and Storage: Store at 4 degree C.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human skin stained with 14.3.3 sigma antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of epidermis.)

Related Product Information for anti-14.3.3 sigma antibody

Mouse monoclonal 14.3.3 Sigma antibody (BSA/Azide Free)

Product Categories/Family for anti-14.3.3 sigma antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 5454052
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142.3
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 26-30 kDa (predicted detection band MW)
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma
• UniProt Protein Name: 14-3-3 protein sigma
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1
• UniProt Entry Name: 1433S_HUMAN

Uniprot Description

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; release of cytochrome c from mitochondria; regulation of epidermal cell division; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; regulation of cyclin-dependent protein kinase activity; negative regulation of caspase activity; signal transduction; positive regulation of cell growth