Rabbit anti-Human, Mouse 14-3-3sigma Polyclonal Antibody | anti-14-3-3sigma antibody

14-3-3sigma (Phospho-Ser186) Antibody

Cat. Num. AAA9416827

• Gene Names: SFN; YWHAS
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: 14-3-3sigma; Polyclonal Antibody; 14-3-3sigma (Phospho-Ser186) Antibody; SFN; 14-3-3 sigma; 14-3-3 protein sigma; HME1; Stratifin; YWHAS; anti-14-3-3sigma antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: 14-3-3sigma (Phospho-Ser186) Antibody detects endogenous levels of 14-3-3sigma only when phosphorylated at Ser186
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-14-3-3sigma antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human 14-3-3sigma (Phospho-Ser186)
• Immunogen Type: Peptide
• Modification: Phospho-Ser186
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis 14-3-3sigma (Phospho-Ser186) using EGF treated HepG2 whole cell lysates)

Product Categories/Family for anti-14-3-3sigma antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5454052
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142.3
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 24,336 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma
• UniProt Protein Name: 14-3-3 protein sigma
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1
• UniProt Entry Name: 1433S_HUMAN

Uniprot Description

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cell-cell adherens junction; cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space

Molecular Function: identical protein binding; protein binding; protein kinase C inhibitor activity

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of caspase activity; negative regulation of protein kinase activity; positive regulation of epidermal cell differentiation; regulation of epidermal cell division; release of cytochrome c from mitochondria; signal transduction