Western Blot (WB)
(Western Blot analysis of whole cell extracts from different cell lines using antibody MBS632037. Samples were electrophoresced usng Novex NuPAGE 10% Bris-Tris gel. Resolved proteins were transferred onto a nitrocellulose membrane and blocked with 5% skim milk. Detection was by chemiluminescence using IgG (HRP) goat anti-mouse secondary antibody and Novex ECL chemiluminescent reagent kit (Pierce Cat. # WP20005).)
Mouse anti-Human 14-3-3, sigma Monoclonal Antibody | anti-SFN antibody
14-3-3, sigma (SFN, Stratifin, HGNC:10773)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Immunoprecipitation: 2ug/mg protein lysate. Use Protein G.
Western Blot: 1ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Western Blot (WB)
(Western Blot analysis of whole cell extracts from different cell lines using antibody MBS632037. Samples were electrophoresced usng Novex NuPAGE 10% Bris-Tris gel. Resolved proteins were transferred onto a nitrocellulose membrane and blocked with 5% skim milk. Detection was by chemiluminescence using IgG (HRP) goat anti-mouse secondary antibody and Novex ECL chemiluminescent reagent kit (Pierce Cat. # WP20005).)
Western Blot (WB)
(Western Blot analysis of whole cell extracts from different cell lines using antibody MBS632037. Samples were electrophoresced usng Novex NuPAGE 10% Bris-Tris gel. Resolved proteins were transferred onto a nitrocellulose membrane and blocked with 5% skim milk. Detection was by chemiluminescence using IgG (HRP) goat anti-mouse secondary antibody and Novex ECL chemiluminescent reagent kit (Pierce Cat. # WP20005).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human skin using antibody MBS632037. Peroxidase-conjugated secondary antibody and AEC staining were used for visualization. Note cytoplasmic staining of epidermis.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human skin using antibody MBS632037. Peroxidase-conjugated secondary antibody and AEC staining were used for visualization. Note cytoplasmic staining of epidermis.)
NCBI and Uniprot Product Information
Uniprot Description
14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; cytosol; nucleus
Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding
Biological Process: positive regulation of protein export from nucleus; release of cytochrome c from mitochondria; regulation of epidermal cell division; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; negative regulation of caspase activity; regulation of cyclin-dependent protein kinase activity; signal transduction; positive regulation of cell growth
Research Articles on SFN
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Product Notes
The SFN sfn (Catalog #AAA632037) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 14-3-3, sigma (SFN, Stratifin, HGNC:10773) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 14-3-3, sigma can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Dilution: Immunoprecipitation: 2ug/mg protein lysate. Use Protein G. Western Blot: 1ug/ml for 2 hours at RT. Immunohistochemistry (Formalin/paraffin): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the SFN sfn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "14-3-3, sigma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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