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738 results for " MAPK Erk Pathway" - showing 1-50
Testing Data
MEK1/2 (Ser218,222), Polyclonal Antibody (Cat# AAA502074)
Full Name
Anti-Phospho-Ser218,222 MEK 1/2
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Mouse
Applications
WB
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Testing Data
MEK1 (Thr292), Polyclonal Antibody (Cat# AAA502072)
Full Name
Anti-Phospho-Thr292 MEK 1
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Recombinant
Applications
WB
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Uterine Carcinoma stained with MAP3K1 Monoclonal Antibody (2F6).)
MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1), Monoclonal Antibody (Cat# AAA439590)
Full Name
MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1) Mouse Monoclonal Antibody
Gene Names
MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
Reactivity
Human. Others not known
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
MEK1 (Thr386), Polyclonal Antibody (Cat# AAA502073)
Full Name
Anti-Phospho-Thr386 MEK 1
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Rat, human
Applications
WB
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from A549, MCF7, C6 and Hela cell lysates using Shc mouse mAb (1:1000 diluted).Predicted band size:63KDa.Observed band size:46, 52, 66KDa.)
Shc, Monoclonal Antibody (Cat# AAA476595)
Full Name
Anti-Shc Mouse mAb
Gene Names
SHC1; SHC; SHCA
Reactivity
Human, Rat
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Fig-1: Induction of SRE activity by FBS or FBS plus PMA in SRE Leeporter? ? HEK293 cells.)
SRE, Cell Line (Cat# AAA668875)
Full Name
SRE Leeporter HEK293 Cell Line
Applications
Functional Assay
Pricing
$2,845/1 Vial | $12,730/5x1 Vial
Western Blot (WB)
(Western blot detection of GRB2 in K562, Raji, Jurkat, Ramos, C6, 3T3 and Hela cell lysates using GRB2 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.)
GRB2, Monoclonal Antibody (Cat# AAA475234)
Full Name
Anti-GRB2 Mouse mAb
Gene Names
GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of OPRM1 Antibody (Center) in mouse heart tissue lysates (35ug/lane). OPRM1 (arrow) was detected using the purified Pab.)
OPRM1, Polyclonal Antibody (Cat# AAA9208620)
Full Name
OPRM1 Antibody (Center)
Gene Names
OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
Reactivity
Human, mouse
(Predicted Reactivity: Monkey, Pig, Rat)
(Predicted Reactivity: Monkey, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot
(Western Blot analysis using MAP4K4 antibodyWestern Blot showing MAP4K4 antibody used against truncated Trx-MAP4K4 recombinant protein (1), MBP-MAP4K4 (aa300-400) recombinant protein (2) and MAP4K4 (aa194-436)-hIgGFc transfected CH0-K1 cell lysate (3).)
MAP4K4, Monoclonal Antibody (Cat# AAA830965)
Full Name
MAP4K4 antibody
Gene Names
MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
Applications
EIA, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Test Data
(Anti-MAP3KI5 Antibody (N -term) at 1:1000 dilution + human fetal brain lysate Lysates/proteins at 20 I1g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 147 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
MAP3K15, Polyclonal Antibody (Cat# AAA9201039)
Full Name
MAP3K15 Antibody (N-term)
Gene Names
MAP3K15; ASK3; bA723P2.3
Reactivity
Human, mouse
Applications
EIA, IHC, FC/FACS, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot
(Western Blot analysis using MAP4K4 antibodyWestern Blot showing MAP4K4 antibody used against truncated Trx-MAP4K4 recombinant protein (1), MBP-MAP4K4 (aa300-400) recombinant protein (2) and MAP4K4 (aa194-436)-hIgGFc transfected CH0-K1 cell lysate (3).)
MAP4K4, Monoclonal Antibody (Cat# AAA831034)
Full Name
MAP4K4 antibody
Gene Names
MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
Applications
EIA, WB
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Western Blot (WB)
(Western blot analysis of anti-ERK5 C-term Pab in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.)
MAPK7, Polyclonal Antibody (Cat# AAA9213301)
Full Name
MAPK7 Antibody (C-term)
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
( Western blot analysis of lysates from 293 cell line, untreated or treated with TPA, 200nM, 30min, using MAP4K1-pS171 (upper) or Tubulin (lower).)
Phospho-MAP4K1 (S171), Polyclonal Antibody (Cat# AAA9201545)
Full Name
Phospho-MAP4K1 (S171) Antibody
Gene Names
MAP4K1; HPK1
Reactivity
Human (Predicted: Mouse)
Applications
WB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
MAP3K8, Polyclonal Antibody (Cat# AAA540421)
Full Name
MAP3K8 Antibody
Gene Names
MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
Reactivity
Human, Mouse, Rat
Applications
EIA, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Western Blot (WB)
()
RSK3, Monoclonal Antibody (Cat# AAA833537)
Full Name
RSK3 antibody
Applications
WB
Purity
RSK3 antibody was purified by ammonium sulfate precipitation
Testing Data
Erk1/2 (MAPK), Monoclonal Antibody (Cat# AAA670164)
Full Name
Mouse Anti-Human Erk1/2 (MAPK)-UNLB
Reactivity
Human
Applications
Immunoprecipitation; Western Blot
Pricing
$285/0.5 mg | $1,145/5x0.5 mg
Mek1, Polyclonal Antibody (Cat# AAA835593)
Full Name
Phospho-Mek1 antibody
Gene Names
MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
Applications
IF, WB
Purity
Phospho-Mek1 antibody was purified by affinity chromatography
Pricing
$625/0.1 mL | $2,795/5x0.1 mL
Western Blot (WB)
(Western blot of rat testes lysate showing specific labeling of the ~49k MEK5 protein (Control))
MAP2K5, Polyclonal Antibody (Cat# AAA835495)
Full Name
MAP2K5 antibody (Ser311, Thr315)
Gene Names
MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
Applications
WB
Purity
MAP2K5 antibody was purified by affinity chromatography
Pricing
$640/0.1 mL | $2,870/5x0.1 mL
Western Blot (WB)
(Western Blot showing p44/42 MAPK antibody used against Jurkat (1), Hela (2), A431 (3) and NIH/3T3 (4) cell lysate.)
ERK1/2, Monoclonal Antibody (Cat# AAA830810)
Full Name
ERK1/2 antibody
Applications
EIA, FC/FACS, WB
Pricing
$565/0.1 mL | $2,540/5x0.1 mL
Western Blot (WB)
()
MEK3, Monoclonal Antibody (Cat# AAA832113)
Full Name
MEK3 antibody
Applications
WB
Purity
MEK3 antibody was purified by IgG purification
Erk1/2 (MAPK), Monoclonal Antibody (Cat# AAA670166)
Full Name
Mouse Anti-Human Erk1/2 (MAPK)-BIOT
Reactivity
Human
Applications
Immunoprecipitation; Western Blot
Pricing
$300/0.5 mg | $1,200/5x0.5 mg
Western Blot (WB)
()
MEK6, Monoclonal Antibody (Cat# AAA832830)
Full Name
MEK6 antibody
Gene Names
MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
Applications
EIA, IP
Purity
MEK6 antibody was purified by ammonium sulfate precipitation
Erk1/2 (MAPK), Monoclonal Antibody (Cat# AAA670165)
Full Name
Mouse Anti-Human Erk1/2 (MAPK)-HRP
Reactivity
Human
Applications
Immunoprecipitation; Western Blot
Pricing
$305/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western blot detection of MEK1/2 in A549, MCF7 and Hela cell lysates using MEK1/2 mouse mAb (dilution 1:500).Predicted band size:45kDa.Observed band size:45kDa.)
MEK1/2, Monoclonal Antibody (Cat# AAA475421)
Full Name
Anti-MEK1/2 Mouse mAb
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB)
(Western blot of human T47D cells showing specific immunolabeling of ~45k MEK 1 (Control))
MAP2K1, Polyclonal Antibody (Cat# AAA837308)
Full Name
MAP2K1 antibody (Thr386)
Applications
WB
Purity
MAP2K1 antibody was purified by affinity chromatography
Pricing
$640/0.1 mL | $2,870/5x0.1 mL
Western Blot (WB)
(Western blot analysis of p44/42 MAPK using rat kidney tissue lysate (Lane 1), mouse 3T3 cell lysate (Lane 2) and Jurkat call lysate (Lane 3 & 4).)
Erk1/2 MAPK, Polyclonal Antibody (Cat# AAA835230)
Full Name
Erk1/2 MAPK antibody
Applications
WB
Purity
Erk1/2 MAPK antibody was purified by affinity chromatography
Pricing
$480/0.1 mg | $2,145/5x0.1 mg
MAP Kinase, Monoclonal Antibody (Cat# AAA832788)
Full Name
MAP Kinase antibody
Applications
WB, IHC, ICC
Pricing
$660/0.1 mg | $2,965/5x0.1 mg
Western Blot (WB)
()
MEK6, Monoclonal Antibody (Cat# AAA834837)
Full Name
MEK6 antibody
Gene Names
MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
Applications
EIA, IP
Purity
MEK6 antibody was purified by ammonium sulfate precipitation
Western Blot (WB)
()
MEK2, Monoclonal Antibody (Cat# AAA838190)
Full Name
MEK2 antibody
Applications
WB, IP
Purity
MEK2 antibody was purified by ammonium sulfate precipitation
Western Blot (WB)
(Western blot analysis of MEKK3 expression in Hela cell lysate (MBS179973).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K3 monoclonal antibody (MBS179973) overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K3 )
MEKK3, Monoclonal Antibody (Cat# AAA179973)
Full Name
Anti-MEKK3 Rabbit Monoclonal Antibody
Gene Names
MAP3K3; MEKK3; MAPKKK3
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
MAPK1, Polyclonal Antibody (Cat# AAA1490658)
Full Name
Rabbit Anti Human MAPK1 PolyClonal Antibody
Gene Names
MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$325/0.1 mL | $1,465/5x0.1 mL
MAP3K4, Polyclonal Antibody (Cat# AAA542036)
Full Name
MAP3K4 Antibody
Gene Names
MAP3K4; MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412
Reactivity
Human, Mouse, Rat
Applications
EIA, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Erk5, Polyclonal Antibody (Cat# AAA839727)
Full Name
Erk5 antibody
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Applications
WB
Purity
Erk5 antibody was purified by affinity purification.
Epithelial Membrane Antigen (MUC1) (ABT-EMA), Monoclonal Antibody (Cat# AAA8541235)
Full Name
Epithelial Membrane Antigen (MUC1) (ABT-EMA) Mouse mAb
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Applications
IHC, WB
Purity
Affinity Chromatography
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Erk5, Polyclonal Antibody (Cat# AAA839709)
Full Name
Erk5 antibody
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Applications
WB
Purity
Erk5 antibody was purified by affinity chromatography
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Mitogen Activated Protein Kinase Kinase Kinase 1 (MAP3K1), Antibody Pair Kit (Cat# AAA2099965)
Full Name
Human Mitogen Activated Protein Kinase Kinase Kinase 1 (MAP3K1) Antibody Pair Kit (with Standard)
Gene Names
MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
Reactivity
Human
Applications
EIA, CLIA
Pricing
$1,470/10x96 Tests | $925/5x96 Tests | $1,000/5x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 5x96 Tests) | $1,595/10x96 Tests + MBS2090685 (Ab Pairs Support Pack 1, 10x96 Tests)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human bladder, using ERK1 Antibody(MBS179109)MAPK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK3 Antibody (MBS179109)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
ERK1, Monoclonal Antibody (Cat# AAA179109)
Full Name
Anti-ERK1 Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ERK1 Antibody(MBS179107)MAPK3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK3 Antibody (MBS179107)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
ERK1, Monoclonal Antibody (Cat# AAA179107)
Full Name
Anti-ERK1 Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-MEK1 (S298) expression in (1) HeLa cell treated with LP lysate; (2) HeLa cell lysate (MBS1750230).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP2K1 )
MEK1, Monoclonal Antibody (Cat# AAA1750230)
Full Name
Anti-Phospho-MEK1 (S298) Rabbit Monoclonal Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (MBS1750208).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK3 )
Erk1, Monoclonal Antibody (Cat# AAA1750208)
Full Name
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MEK5 expression in Hela cell lysate (MBS179884).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP2K7 )
MEK7, Monoclonal Antibody (Cat# AAA179884)
Full Name
Anti-MEK7 Rabbit Monoclonal Antibody
Gene Names
MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho- Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (MBS1750209).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK3 )
Erk1, Monoclonal Antibody (Cat# AAA1750209)
Full Name
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MEKK2 expression in HeLa cell lysate (MBS179832).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K2 )
MEKK2, Monoclonal Antibody (Cat# AAA179832)
Full Name
Anti-MEKK2 Rabbit Monoclonal Antibody
Gene Names
MAP3K2; MEKK2; MEKK2B
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody(MBS179244)MAP2K1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K1 Antibody (MBS179244)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
MEK1/2, Monoclonal Antibody (Cat# AAA179244)
Full Name
Anti-MEK1/2 Rabbit Monoclonal Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ERK5 expression in Hela cell lysate (MBS179898).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK7 )
ERK5, Monoclonal Antibody (Cat# AAA179898)
Full Name
Anti-ERK5 Rabbit Monoclonal Antibody
Gene Names
MAPK7; BMK1; ERK4; ERK5; PRKM7
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MEK1 expression in (1) A431 cell lysate;(2) HeLa cell lysate (MBS179245).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP2K1 )
MEK1, Monoclonal Antibody (Cat# AAA179245)
Full Name
Anti-MEK1 Rabbit Monoclonal Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
WWC1 / Protein KIBRA, Recombinant Protein (Cat# AAA2903357)
Full Name
Human WWC1 / Protein KIBRA Recombinant Protein
Gene Names
WWC1; KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates (MBS179108).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK3 )
ERK1/2, Monoclonal Antibody (Cat# AAA179108)
Full Name
Anti-ERK1/2 Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of MEK6 using anti-MEK6 antibody (MBS1750738).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysate,Lane 2: mouse skeletal muscle tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK6 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MEK6 at approximately 38KD. The expected band size for MEK6 is at 38KD. )
MEK6, Polyclonal Antibody (Cat# AAA1750738)
Full Name
Anti-MEK6 Picoband Antibody
Gene Names
MAP2K6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
MAPK3 / Mitogen-Activated Protein Kinase 3, Recombinant Protein (Cat# AAA2902460)
Full Name
Human MAPK3 / Mitogen-Activated Protein Kinase 3 Recombinant Protein
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
