WWC1 / Protein KIBRA Recombinant Protein | WWC1 recombinant protein

Human WWC1 / Protein KIBRA Recombinant Protein

• Gene Names: WWC1; KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: WWC1 / Protein KIBRA; Human WWC1 / Protein KIBRA Recombinant Protein; Protein KIBRA; HBeAg-binding protein 3; Kidney and brain protein; KIBRA; WW domain-containing protein 1; KIAA0869; WWC1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 1119

• Applicable Applications for WWC1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Homodimer. Interacts with DDN. Interacts with DYNLL1 and histone H3. The interaction with DYNLL1 is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin and the interaction with histone H3 ensures proper regulatory interaction of WWC1-DYNLL1-ESR1 complexes with target chromatin. Interacts (via WW domains) with DDR1 (via PPxY motif) in a collagen-regulated manner. Interacts with PRKCZ (via the protein kinase domain). Forms a tripartite complex with DDR1 and PRKCZ, but predominantly in the absence of collagen. Interacts (via the ADDV motif) with PATJ (via PDZ domain 8). Interacts (via WW domains) with SYNPO (via PPxY motifs). Interacts with NF2 and SNX4.
• Entry Function: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 242247251
• NCBI GeneID: 23286
• NCBI Accession #: NP_001155133.1
• NCBI GenBank Nucleotide #: NP_001155133.1
• UniProt Accession #: Q8IX03
• NCBI Official Full Name: protein KIBRA isoform 1
• NCBI Official Synonym Full Names: WW and C2 domain containing 1
• NCBI Official Symbol: WWC1
• NCBI Official Synonym Symbols: KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
• NCBI Protein Information: protein KIBRA
• UniProt Protein Name: Protein KIBRA
• UniProt Gene Name: WWC1
• UniProt Synonym Gene Names: KIAA0869; KIBRA
• UniProt Entry Name: KIBRA_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

KIBRA: a postsynaptic scaffolding protein associated with human memory performance. Interacts via its WW domains with the postsynaptic protein DDN, which plays a role in synaptic plasticity. Expressed in the hypothalamus and cerebellum, and in the hippocampus and cortex, both regions of the brain that are related to memory. Co-localizes with PKCZ in the mouse hippocampus. Binds to synaptopodin, modulating podocyte directional migration. Forms a complex with dynein light chain 1 that is recruited to ER-responsive promoters, and that is required for ER transactivation in breast cancer cells. Binds the minus end-directed microtubule motor dynein. Its amino terminus binds the eight PDZ domain of the polarity protein INADL . Two human isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: protein complex; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding, bridging; protein binding; transcription coactivator activity; protein complex scaffold; kinase binding

Biological Process: cell migration; regulation of intracellular transport; positive regulation of MAPKKK cascade; transcription, DNA-dependent; establishment of cell polarity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of organ growth

Disease: Memory Quantitative Trait Locus