Western Blot (WB)
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Mouse MEK2 Monoclonal Antibody | anti-MEK2 antibody
MEK2 antibody
Applications
Western Blot, Immunoprecipitation
Purity
MEK2 antibody was purified by ammonium sulfate precipitation
Synonyms
MEK2; Monoclonal Antibody; MEK2 antibody; Monoclonal MEK2; Anti-MEK2; MAP2K2; mitogen-activated protein kinase kinase 2; BOS_7688; dual specificity mitogen activated protein kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2; MAP kinase/Erk kinase; MAP kinase kinase 2; MAPKK2; MAPKK 2; MEK 2; mitogen activated protein kinase kinase 2; p45; mitogen-activated protein kinase kinase type 2; MK2; MKK2; PRKMK2; protein kinase; mitogen activated; kinase 2; anti-MEK2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
1A1
Purity/Purification
MEK2 antibody was purified by ammonium sulfate precipitation
Form/Format
Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
Applicable Applications for anti-MEK2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1: 2000
IP: 2 ul
IP: 2 ul
Biological Significance
The ERK (extracellular-signal regulated kinase) cascade is a central MAPK pathway which contains Ras as a G protein, Raf as a MAPKKK, MEK1 (MAPK/ERK kinase1) and MEK2 (MAPK/ERK kinase2) as MAPKK, and ERKs as MAPK. MEK1 and MEK2 are 80% identical to each other, and are essentially identical in most of their kinase domain. MEK1 and MEK2 phosphorylate ERKs equally well, both in vivo and in vitro. MEK1 (45 kDa) and MEK2 (46 kDa) are composed of a catalytic kinase domain, which is surrounded by a regulatory N-terminal domain (80 amino acids) and a shorter C-terminal region (30 amino acids). Unlike the kinase domains, the N-termini and the characteristic Pro-rich inserts are quite divergent between the two MEKs (40% identity). MEKs are activated by phosphorylation of two Ser residues in their activation loop (Ser218 and Ser222 in MEK1) located within a Ser?Xaa?Ala?Xaa?Ser/Thr motif, typical to all MAPKKs.
Cross-Reactivity
Human
Immunogen
MEK2 antibody was raised in Mouse using a recombinant human protein purified from E Coli (His-MEK2) as the immunogen
Preparation and Storage
Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles
Western Blot (WB)
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Western Blot (WB)
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Related Product Information for anti-MEK2 antibody
Mouse monoclonal MEK2 antibody
Product Categories/Family for anti-MEK2 antibody
Similar Products
Product Notes
The MEK2 (Catalog #AAA838190) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1: 2000 IP: 2 ul. Researchers should empirically determine the suitability of the MEK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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