Western Blot (WB) (All lanes: BAG family molecular chaperone regulator 2 antibody at 6ug/mlLane 1:Jurkat whole cell lysateLane 2:Hela whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 24,21 kDaObserved band size: 24 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS1492793 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS1492793 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: BAG family molecular chaperone regulator 5 antibody at 2ug/mlLane 1:mouse kidney tissueLane 2:mouse brain tissueLane 3:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52,57 kDaObserved band size: 51 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7003400 at dilution of 1:100)
Western Blot (WB) (All lanes: BAG family molecular chaperone regulator 5 antibody at 1ug/mlLane 1:Hela whole cell lysateLane 2:jurkat whole cell lysateLane 3:A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52,57 kDaObserved band size: 51 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1497693 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectum using MBS1497693 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using MBS1497693 at dilution 1:100)
Immunoprecipitation (IP) (Immunoprecipitating Bag3 in K562 whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS1495442 in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS1495442 (6ug)+ K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (10ug))
Western Blot (WB) (Western BlotPositive WB detected in:Jurkat whole cell lysateAll lanes: MCL1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38,29 kDaObserved band size: 38 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7049428 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of MCF-7 cells using MBS7049428 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7049428 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7047741 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells using MBS7047741 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in: Mouse liver tissueAll lanes: BAG4 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50,46 KDaObserved band size: 50 KDa)
Western Blot (WB) (Western blot analysis of anti-Cyclin D1 Antibody (S90) in mouse lung lysates (35ug/lane). Cyclin D1(arrow) was detected using the purified Pab.)
Western Blot (WB) (All lanes : Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution Lane 1: MCF-7 whole cell lysateLane 2: A431 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Western Blot (WB) (All lanes : Anti-Cyclin D1 Antibody (S90) at 1:2000 dilution Lane 1: HT-1080 whole cell lysateLane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Cyclin D1 Antibody (S90), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human Testis tissue reacted with Cyclin D1 Antibody (S90) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) (MBS9214117 staining Cyclin D1 in human tonsil tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of Cyclin D1 Antibody (S90) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
Western Blot (WB) (Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of BAG 1 in mouse brain tissue with BAG 1 Antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of BAG 1 in mouse brain tissue with BAG 1 Antibody at 20 μg/mL.Green: BAG 1 antibody (3869)Red: Phylloidin stainingBlue: DAPI staining)
Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde-fixed A431, using BCL2L10 antibody at 1:200 dilution.)
Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 12 % SDS PAGE gel and BclB antibody at a dilution of 1:1000)
Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded A549 Xenograft using BclB antibody at a dilution of 1:100)
Western Blot (WB) (Western blot detection of Beclin 1 in Hela, 3T3, C6, COS7, CHO-K1, Rat brain and Jurkat cell lysates using Beclin 1 mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:55KDa.)
Immunohistochemistry (IHC) (Anti-BCL2A1 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of Bfl-1 in Mouse Kidney cells with Bfl-1 antibody at 20 ug/ml.)
Western Blot (WB) (Western blot of Bfl-1 in human kidney tissue lysate with Bfl-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)
Western Blot (WB) (Fig: 1 Western blot analysis of Bid using at 1: 2000. Detected in vitro translated Bid, but not in vitro translated Bcl-2, Bcl-XL. Bax, or Bad. These results show that is specific for Bid and does not crossreact with other Bcl-2 family members)
Immunohistochemistry (IHC) (Fig: 2 Formalin-fixed, paraffin-embedded mouse embryos stained for Bid using at 1: 2000. A. Intrauterine embryo at E.95 dpc (days post conception). B. 10.5-11 dpc embryo isolated from the yolk sac. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC) (Fig: 3 Formalin-fixed, paraffin-embedded human tissue sections stained for Bid using at 1: 2000. A and A1, Adult pancreas. B and B1, Adult mesencephalon (midbrain). A1 and B1 are higher magnifications of A and B, respectively. Hematoxylin-eosin counterstain.)
Western Blot (WB) (Western blot detection of Bax in COS7, Raji, CEM and Hela cell lysates using Bax mouse mAb (1:1000 diluted). Predicted band size:20KDa. Observed band size:20KDa.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: breast carcinoma. Species: Human. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:100.)
Western Blot (WB) (Western blot analysis of Human K562 cells lysates showing detection of PUMA protein using Rabbit Anti-PUMA Polyclonal Antibody . Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:500.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-PUMA Polyclonal Antibody . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-PUMA Polyclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)
Western Blot (WB) (Western blot analysis of Bag 1 expression in HeLa cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9601309 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9601309 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at104)
Western Blot (WB) (Fig: 1 Western blot analysis of BAG-3 using at 1: 2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.)
Immunohistochemistry (IHC) (Fig: 2 Immunohistochemistry-Paraffin: BAG3 Antibody. Staining of human pancreas tissue sections using this antibody at 1: 2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC) (Fig: 3Frozen mouse muscle tissue section stained for BAG-3 expression using at 1: 2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.)
Western Blot (WB) (Western blot analysis of BAG2 expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using BAG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney cancer using BAG1 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney injury using BAG1 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG1 Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human esophagus using BAG1 Antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cells using BAG1 antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using BAG3 antibody.)
Immunohistochemistry (IHC) (MBS9606222 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from mouse brain, using BAG3 Antibody.)
Western Blot (WB) (Western blot analysis on COS7 cell lysate using MCL1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (IHC analysis of mouse colon tissue, using MCL1 Antibody at 1:100.)
Immunofluorescence (IF) (MBS9600143 staining U-2 OS cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB) (Western blot analysis of SODD in HeLa (1,3) and THP-1 (2,4) whole cell lysates in the absence (1,2) or presence (3,4) of blocking peptide with SODD antibody at 1:500 dilution.)
Immunocytochemistry (IHC) (Immunocytochemistry of SODD in HeLa cells with SODD antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of SODD in Hela cells with SODD antibody at 20 μg/mL.)
Western Blot (WB) (Western blot analysis of BAGE5 using HeLa whole cell lysates)
Immunofluorescence (IF) (MBS9606223 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of extracts from K562 cells, using BAG4 antibody.)
Immunohistochemistry (IHC) (MBS9606223 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis on rat spleen tissue lysate using MCL1 Antibody)
Immunofluorescene (IF) (MBS9601483 staining LOVO cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9601483 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF) (MBS9601483 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC) (MBS9601483 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9601483 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC) (MBS9601483 at 1/200 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 μg/mL)
Immunofluorescence (IF) (Immunofluorescence of BAG-1 in Human Lymph Node cells with BAG-1 antibody at 20 μg/mL.)
Western Blot (WB) (Western blot analysis of BCL7B expression in Y79 cell line lysates, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (Anti-BCL2A1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF) (Immunofluorescence of Bfl-1 in Mouse Kidney cells with Bfl-1 antibody at 20 ug/ml.)
Western Blot (WB) (Western blot of Bfl-1 in mouse kidney tissue lysate with Bfl-1 antibody at (A) 1 and (B) 2 ug/ml.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-Bcl-2 Polyclonal Antibody . Tissue: Colon. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-Bcl-2 Polyclonal Antibody . Tissue: Colon. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.)
Western Blot (WB) (Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~26.3 kDa Bcl-2 protein using Rabbit Anti-Bcl-2 Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Bcl-2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~26.3 kDa.)
Western Blot (WB) (Gel: 12%SDS-PAGE Lysate: 40 μ g Lane 1-5: A549£¬A431,Hela£¬Jurkat and HEPG2 cell Primary antibody: 1/300 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/25.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/25.)
Immunohistochemistry (IHC) (MBS9606904 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of NCI-H460, using BAG1 antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB) (Western blot analysis of BAG2 using Jurkat whole lysates.)
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