Western Blot (WB)
(MIC1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIC1 antibody detected the MIC1 protein (arrow).)
Rabbit anti-Human MIC1 Polyclonal Antibody | anti-MIC1 antibody
MIC1, NT (C18orf8, MIC1, Uncharacterized protein C18orf8, Colon cancer-associated protein Mic1) (PE)
Gene Names
C18orf8; MIC1; Mic-1; HsT2591
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
MIC1; Polyclonal Antibody; NT (C18orf8; Uncharacterized protein C18orf8; Colon cancer-associated protein Mic1) (PE); C18orf8; Colon cancer-associated protein Mic1; anti-MIC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MIC1 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IF: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IF: 1:10-50
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
MIC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-74 amino acids from the N-terminal region of human MIC1.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(MIC1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIC1 antibody detected the MIC1 protein (arrow).)
Western Blot (WB)
(MIC1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIC1 antibody detected the MIC1 protein (arrow).)
Flow Cytometry (FC/FACS)
(MIC1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MIC1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MIC1 Antibody (N-term) with MDA-MBA231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MIC1 Antibody (N-term) with MDA-MBA231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-MIC1 antibody
References
Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,975 Da
NCBI Official Full Name
uncharacterized protein C18orf8 isoform 1
NCBI Official Synonym Full Names
chromosome 18 open reading frame 8
NCBI Official Symbol
C18orf8
NCBI Official Synonym Symbols
MIC1; Mic-1; HsT2591
NCBI Protein Information
uncharacterized protein C18orf8; colon cancer-associated protein Mic1
UniProt Protein Name
Uncharacterized protein C18orf8
Protein Family
UniProt Gene Name
C18orf8
UniProt Synonym Gene Names
MIC1; Mic-1
UniProt Entry Name
MIC1_HUMAN
NCBI Description
This gene encodes a colon cancer associated protein. [provided by RefSeq, Jan 2013]
Uniprot Description
C18orf8: Belongs to the Mic1 family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 18q11.2
Cellular Component: lysosomal membrane
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Product Notes
The MIC1 c18orf8 (Catalog #AAA6320722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIC1, NT (C18orf8, MIC1, Uncharacterized protein C18orf8, Colon cancer-associated protein Mic1) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIC1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IF: 1:10-50 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MIC1 c18orf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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