Human BCL2-associated athanogene 4 ELISA Kit | BAG4 elisa kit

Human BAG family molecular chaperone regulator 4, BAG4 ELISA Kit

Cat. Num. AAA9339475

• Gene Names: BAG4; SODD; BAG-4
• Reactivity: Human
• Synonyms: BCL2-associated athanogene 4; Human BAG family molecular chaperone regulator 4; BAG4 ELISA Kit; Human BAG family molecular chaperone regulator 4 (BAG4) ELISA kit; BAG-4; SODD; BAG-family molecular chaperone regulator-4; silencer of death domains; BAG4 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human BAG4 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 ng/ml - 1000 ng/ml.
• Sensitivity: 5.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated BAG4 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAG4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAG4 and BAG4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for BAG4 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated BAG4 concentrations in Human serum, plasma and other body fluids. Using Purified Human BAG4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add BAG4 and BAG4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BAG4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 325910869
• NCBI GeneID: 9530
• NCBI Accession #: NP_001191807.1
• NCBI GenBank Nucleotide #: NM_001204878.1
• UniProt Accession #: O95429; O95818; B4E217
• Molecular Weight: 49,594 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 4 isoform 2
• NCBI Official Synonym Full Names: BCL2-associated athanogene 4
• NCBI Official Symbol: BAG4
• NCBI Official Synonym Symbols: SODD; BAG-4
• NCBI Protein Information: BAG family molecular chaperone regulator 4; silencer of death domains; bcl-2-associated athanogene 4; BAG-family molecular chaperone regulator-4
• UniProt Protein Name: BAG family molecular chaperone regulator 4
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG4
• UniProt Synonym Gene Names: SODD; BAG-4
• UniProt Entry Name: BAG4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

BAG4: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Prevents constitutive TNFRSF1A signaling. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; positive regulation of actin filament polymerization; protein folding; protein heterooligomerization; positive regulation of stress fiber formation; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis