Immunohistochemistry (IHC) (IHC image of MBS1496770 diluted at 1:800 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (IHC image of MBS1496770 diluted at 1:800 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (Immunofluorescence staining of HepG2 cells with MBS1496770 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Western Blot (WB) (All lanes: Charged multivesicular body protein 2a antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 24 kDaObserved band size: 16kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human placenta using MBS715743 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS1493383 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1493383 at dilution of 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: Rat lung tissueAll lanes: CHMP6 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 24 KDaObserved band size: 24 KDa)
Western Blot (WB) (Western BlotPositive WB detected in: Mouse brain tissueAll lanes: VPS26B antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 40 KDaObserved band size: 40 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of human colon cancer using MBS1498295 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of PC3 cells using MBS1498295 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: VPS33B antibody at 2.72 ug/mlLane 1: Hela whole cell lysateLane 2: A549 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 71,61 kDaObserved band size: 71,51,60 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7043823 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7043823 at dilution of 1:100)
Immunofluorescence/Immunocytochemistry (IF/ICC) (MBS9604763 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
Immunohistochemistry (IHC) (MBS9604763 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis on mouse brain lysates using VPS72 Antibody)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7052497 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells using MBS7052497 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western BlotPositive WB detected in: Mouse heart tissue,Mouse brain tissueAll lanes: VPS4B antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 50 KDaObserved band size: 50 KDa)
Western Blot (WB) (Western blotAll lanes: VPS45 antibody at 3ug/mlLane 1: Mouse brain tissueLane 2: U87 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 66,62 kDaObserved band size: 41 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7003340 at dilution of 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: MCF-7 whole cell lysate,HepG2 whole cell lysate,Hela whole cell lysate,Mouse heart tissue,Mouse brain tissue,Rat liver tissue,Rat kidney tissueAll lanes: VPS29 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 21 KDaObserved band size: 28 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7053341 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of PC3 cells using MBS7053341 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..)
Immunoprecipitation (IP) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)
Western Blot (WB) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)
Western Blot (WB) (Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
Western Blot (WB) (Western blot analysis of CHMP4B Antibody (N-term) in SK-BR-3 cell line lysates (35ug/lane).CHMP4B (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CHMP4B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (CHMP4B Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ATP6V1B1 protein (Right) or empty vector (Left) detected with ATP6V1B1 antibody)
Immunohistochemistry (IHC) (Immunohistochemical analysis of ATP6V1B1 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using ATP6V1B1 antibody)
Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP6V1B1 protein using ATP6V1B1 antibody)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 5A9 depletes VPS35 from the A549 cell extract..)
Immunoprecipitation (IP) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)
Western Blot (WB) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)
Western Blot (WB) (Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
Western Blot (WB) (Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 μg/ml.Green: VPS39 antibody (6183)Red: Phylloidin stainingBlue: DAPI staining)
Western Blot (WB) (Western blot analysis of extracts of mouse heart tissue, using CHMP4B antibody.)
Immunohistochemistry (IHC) (MBS9603484 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using CHMP4B antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: Rat brain; Lane 3: Mouse brain; )
SDS-Page
Western Blot (WB) (Western blot analysis of extracts from Mouse brain tissue, using VPS39 Antibody. The lane on the left was treated with blocking peptide.)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..)
Immunoprecipitation (IP) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)
Western Blot (WB) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)
Western Blot (WB) (Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..)
Immunoprecipitation (IP) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)
Western Blot (WB) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)
Western Blot (WB) (Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
Immunohistochemistry (IHC) (MBS9603375 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using CHMP2A antibody.)
Western Blot (WB) ((0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) (staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3ug/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining.)
Immunofluorescence (IF) (staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.)
Immunofluorescence (IF) (staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.)
Western Blot (WB) (Western blot analysis of VPS54 using HepG2 whole cell lysates)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CHMP2B antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human thyroid cancer using CHMP2B antibody at dilution of 1:200 (400x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cell using CHMP2B antibody. Blue: DAPI for nuclear staining.)
Immunohistochemistry (IHC) (MBS9603409 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of HeLa cells, using VPS33B antibody.)
Western Blot (WB) (Western blot analysis of extracts of human spleen, using CHMP6 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CHMP1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using CHMP1B antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using CHMP1B antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver injury using CHMP1B antibody at dilution of 1:100 (40x lens).)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..)
Immunoprecipitation (IP) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)
Western Blot (WB) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)
Western Blot (WB) (Western Blot analysis of Human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
Western Blot (WB) (Western blot analysis of Cadherin 24 expression in SHSY5Y whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of VPS72 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF) (Immunofluorescent analysis of VPS72 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Testing Data (Sample(30 ug whole cell lysate)A: H1299B: HeLa S3C: MOLT4 12% SDS PAGEPrimary antibody diluted at 1: 1000)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using VTA1 antibody at 1: 500 dilution.)
Immunofluorescence (IF) (Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using VTA1 antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 500.)
Western Blot (WB) (Western blot analysis of VPS4B expression in 293T whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC) (MBS9603728 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts of HEK-293 cells, using VPS11 antibody.)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degree C. clone 10A8 depletes VPS35 from the A549 cell extract..)
Immunoprecipitation (IP) (Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.)
Western Blot (WB) (Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.)
Testing Data ()
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of A549 lysate for 2 hours at 4 degree C.)
Immunoprecipitation (IP) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 10A8. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
Western Blot (WB) (Western blot analysis of extracts of mouse liver tissue, using VPS37A antibody.)
Immunohistochemistry (IHC) (MBS9603471 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB) (Western blot analysis of extracts from rat brain, using VPS37A antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB) (Western blot-VPS51 Polyclonal Antibody)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CHMP1A antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.