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177 results for "Rat IgG2b kappa Isotype Control" - showing 1-50
Flow Cytometry (FC/FACS)
(Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.)
CD16/CD32, Recombinant Antibody (Cat# AAA488078)
Full Name
Anti-CD16/CD32 [2.4G2]
Gene Names
Fcgr3; CD16
Reactivity
Mouse
Applications
FC/FACS, IF, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western Blot analysis of mTLR3. Anti- mTLR3 antibody (Clone: ABM24E5) was used at 2 ug/ml on mouse brain lysate.)
mTLR3, Monoclonal Antibody (Cat# AAA668027)
Full Name
Monoclonal Antibody to mTLR3 (ABM24E5)
Gene Names
Tlr3; AI957183
Reactivity
Mouse, Human
Applications
WB, IHC
Purity
Protein G Chromatography
Pricing
$325/0.1 mg | $165/0.025 mg | $1,405/5x0.1 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed mouse splenocytes with anti-CD74 antibody In-1 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of In-1 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD74, Monoclonal Recombinant Antibody (Cat# AAA488365)
Full Name
Anti-CD74 [In-1]
Reactivity
Mouse
Applications
IP, FC/FACS, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR860.103.5 Mouse spleenocyts were stained with an isotype control or the rabbit-chimeric version of YGITR860.103.5 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the leukocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
GITR, Monoclonal Recombinant Antibody (Cat# AAA488307)
Full Name
Anti-GITR [YGITR 860.103.5]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
GITR, Monoclonal Recombinant Antibody (Cat# AAA488333)
Full Name
Anti-GITR [YGITR765]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
SDS-Page
(SDS-PAGE Analysis Purified HER-4/ERBB4 Mouse Monoclonal Antibody (HFR-1). Confirmation of Purity and Integrity of Antibody.)
HER-4/ERBB4, Monoclonal Antibody (Cat# AAA4380869)
Full Name
HER-4/ERBB4
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse
Predicted to work with Rat, Chicken.
Predicted to work with Rat, Chicken.
Applications
FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 Mouse lymphocytes were stained with an isotype control or the rabbit-chimeric version of 7G6 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
Complement Receptor 1 & 2, Recombinant Antibody (Cat# AAA488171)
Full Name
Anti-Complement Receptor 1 & 2 [7G6]
Reactivity
Mouse
Applications
FC/FACS, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
TER-119, Monoclonal Antibody (Cat# AAA2556957)
Full Name
Purified Anti-Mouse TER-119 Monoclonal Antibody
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, IP
Pricing
$130/0.1 mg | $85/0.025 mg | $570/5x0.1 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557870)
Full Name
Rat IgG2b, kappa Isotype Control (PE/Cy5 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
F4/80, Antibody (Cat# AAA2553715)
Full Name
Purified Anti-Mouse F4/80 Monoclonal Antibody
Gene Names
Adgre1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Reactivity
Mouse
Applications
FC, WB, IHC
Pricing
$90/0.05 mg | $105/0.1 mg | $460/5x0.1 mg
CD44, Antibody (Cat# AAA2558661)
Full Name
Anti-Mouse/Human CD44 Monoclonal Antibody (PE/Cyanine7 Conjugated) [IM7]
Gene Names
Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
Reactivity
Human, Mouse
Applications
FCM
Pricing
$155/0.05 mg | $190/0.1 mg | $275/0.2 mg | $125/0.025 mg | $1,230/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557867)
Full Name
Rat IgG2b, kappa Isotype Control (PE Conjugated)
Applications
FC/FACS
Pricing
$125/100 Tests | $145/200 Tests | $115/50 Tests | $90/20 Tests | $645/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557879)
Full Name
Purified Rat IgG2b, kappa Isotype Control
Applications
FC/FACS
Pricing
$85/0.05 mg | $100/0.1 mg | $115/0.2 mg | $75/0.025 mg | $505/5x0.2 mg
CD1d, Monoclonal Antibody (Cat# AAA2558228)
Full Name
AF/LE Purified Anti-Mouse CD1d Monoclonal Antibody
Gene Names
Cd1d1; Cd1a; Cd1d; CD1.1; Ly-38; AI747460
Reactivity
Mouse
Applications
FC/FACS
Pricing
$120/0.05 mg | $225/0.5 mg | $365/1 mg | $1,630/5x1 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557880)
Full Name
Rat IgG2b, kappa Isotype Control (Biotin Conjugated)
Applications
FC/FACS
Pricing
$85/0.05 mg | $100/0.1 mg | $115/0.2 mg | $75/0.025 mg | $505/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557885)
Full Name
Rat IgG2b, kappa Isotype Control (PE/Cy5 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557890)
Full Name
Rat IgG2b, kappa Isotype Control (Alexa Fluor 488 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
Western Blot (WB)
(Western Blot using anti-CD2 antibody YTH 655 Human tonsil lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 655 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD2 is 39.5kDa, but this protein is glycosylated at several residues. successfully detected CD2 in human tonsil lysate.)
CD2, Monoclonal Recombinant Antibody (Cat# AAA488386)
Full Name
Anti-CD2 [YTH 655]
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557868)
Full Name
Rat IgG2b, kappa Isotype Control (APC Conjugated)
Applications
FC/FACS
Pricing
$145/100 Tests | $175/200 Tests | $120/50 Tests | $95/20 Tests | $785/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557884)
Full Name
Rat IgG2b, kappa Isotype Control (PerCP Conjugated)
Applications
FC/FACS
Pricing
$145/0.05 mg | $170/0.1 mg | $225/0.2 mg | $110/0.025 mg | $960/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557871)
Full Name
Rat IgG2b, kappa Isotype Control (PE/Cy7 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557882)
Full Name
Rat IgG2b, kappa Isotype Control (PE Conjugated)
Applications
FC/FACS
Pricing
$85/0.05 mg | $100/0.1 mg | $115/0.2 mg | $75/0.025 mg | $505/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557873)
Full Name
Rat IgG2b, kappa Isotype Control (PerCP/Cy5.5 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557886)
Full Name
Rat IgG2b, kappa Isotype Control (PE/Cy7 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
Unknown Specificity (Isotype control), Recombinant Antibody (Cat# AAA488155)
Full Name
Anti-Unknown Specificity (Isotype control) [MOPC-21]
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557883)
Full Name
Rat IgG2b, kappa Isotype Control (APC Conjugated)
Applications
FC/FACS
Pricing
$90/0.05 mg | $105/0.1 mg | $130/0.2 mg | $85/0.025 mg | $565/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557869)
Full Name
Rat IgG2b, kappa Isotype Control (PerCP Conjugated)
Applications
FC/FACS
Pricing
$160/100 Tests | $210/200 Tests | $125/50 Tests | $100/20 Tests | $935/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557887)
Full Name
Rat IgG2b, kappa Isotype Control (PE/Cy5.5 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557892)
Full Name
Rat IgG2b, kappa Isotype Control (APC/Cy7 Conjugated)
Applications
FC/FACS
Pricing
$145/0.05 mg | $170/0.1 mg | $225/0.2 mg | $110/0.025 mg | $960/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557866)
Full Name
Rat IgG2b, kappa Isotype Control (FITC Conjugated)
Applications
FC/FACS
Pricing
$125/100 Tests | $145/200 Tests | $115/50 Tests | $90/20 Tests | $645/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557878)
Full Name
LE/AF Purified Rat IgG2b, kappa Isotype Control
Applications
FC/FACS
Purity
>95% as determined by SDS-PAGE.
Pricing
$85/0.05 mg | $100/0.1 mg | $115/0.2 mg | $75/0.025 mg | $505/5x0.2 mg
Fluorescein, Recombinant Antibody (Cat# AAA488047)
Full Name
Anti-Fluorescein [4-4-20 (enhanced)]
Applications
WB, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Testing Data
(Anti-CD11b staining of murine neutrophils. Ex vivo murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-CD11b antibody and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-CD11b (hybridoma-derived antibody obtained from competitor, clone M1/70) directly conjugated to PerCP. Comparison to unstained cells shows that that cells analysed are predominantly CD11b-positive. Right panel shows staining first with anti-CD11b) or isotype control followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control, cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive, agreeing with findings obtained using hybridoma-derived antibody.)
CD11b, Monoclonal Recombinant Antibody (Cat# AAA488381)
Full Name
Anti-CD11b [5C6 (Recombinant Version)]
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Mouse, Human
Applications
IF, IHC
Purity
Protein A Affinity Purified
Pricing
$635/0.1 mg | $2,660/5x0.1 mg
Western Blot (WB)
(Western Blot using anti-CD2 antibody YTH 655 Human tonsil lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of YTH 655 (MBS488385) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD2 is 39.5kDa, but this protein is glycosylated at several residues. MBS488385 successfully detected CD2 in human tonsil lysate.)
CD2, Monoclonal Recombinant Antibody (Cat# AAA488385)
Full Name
Anti-CD2 [YTH 655]
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557874)
Full Name
Rat IgG2b, kappa Isotype Control (APC/Cy5.5 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557881)
Full Name
Rat IgG2b, kappa Isotype Control (FITC Conjugated)
Applications
FC/FACS
Pricing
$85/0.05 mg | $100/0.1 mg | $115/0.2 mg | $75/0.025 mg | $505/5x0.2 mg
Testing Data
(Anti-CD11b staining of murine neutrophils. Ex vivo murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-CD11b antibody and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-CD11b (hybridoma-derived antibody obtained from competitor, clone M1/70) directly conjugated to PerCP. Comparison to unstained cells shows that that cells analysed are predominantly CD11b-positive. Right panel shows staining first with anti-CD11b) or isotype control followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control, cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive, agreeing with findings obtained using hybridoma-derived antibody.)
CD11b, Monoclonal Recombinant Antibody (Cat# AAA488382)
Full Name
Anti-CD11b [5C6 (Recombinant Version)]
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Mouse, Human
Applications
IF, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557891)
Full Name
Rat IgG2b, kappa Isotype Control (Alexa Fluor 647 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557875)
Full Name
Rat IgG2b, kappa Isotype Control (Alexa Fluor 488 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557872)
Full Name
Rat IgG2b, kappa Isotype Control (PE/Cy5.5 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557889)
Full Name
Rat IgG2b, kappa Isotype Control (APC/Cy5.5 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557888)
Full Name
Rat IgG2b, kappa Isotype Control (PerCP/Cy5.5 Conjugated)
Applications
FC/FACS
Pricing
$130/0.05 mg | $155/0.1 mg | $195/0.2 mg | $100/0.025 mg | $860/5x0.2 mg
IgG2b, kappa, Isotype Control (Cat# AAA2557877)
Full Name
Rat IgG2b, kappa Isotype Control (APC/Cy7 Conjugated)
Applications
FC/FACS
Pricing
$185/100 Tests | $255/200 Tests | $145/50 Tests | $115/20 Tests | $1,105/5x200 Tests
IgG2b, kappa, Isotype Control (Cat# AAA2557876)
Full Name
Rat IgG2b, kappa Isotype Control (Alexa Fluor 647 Conjugated)
Applications
FC/FACS
Pricing
$165/100 Tests | $225/200 Tests | $125/50 Tests | $100/20 Tests | $995/5x200 Tests
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD73 antibody YDBYT 208.3 Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YDBYT 208.3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD73, Monoclonal Recombinant Antibody (Cat# AAA488417)
Full Name
Anti-CD73 [YDBYT 208.3]
Gene Names
Nt5e; NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
Reactivity
Mouse
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Rat IgG2b Isotype Control, Monoclonal Isotype Control (Cat# AAA679102)
Full Name
Rat IgG2b-FITC
Applications
ELISA; Flow Cytometry
Pricing
$395/0.5 mg | $1,620/5x0.5 mg
Flow Cytometry (FC/FACS)
(Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug Rat IgG2b Isotype Control (open histogram) or 0.25 ug of CD279 antibody (filled histogram) followed by Anti-Rat IgG (biotin) and Streptavidin (PE). Total viable cells were used for analysis.)
PD1, Monoclonal Antibody (Cat# AAA832832)
Full Name
PD1 antibody
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Applications
Functional Assay
Purity
PD1 antibody was purified by affinity chromatography.
Pricing
$500/0.1 mg | $2,235/5x0.1 mg
IL-4, Monoclonal Antibody (Cat# AAA670066)
Full Name
Rat Anti-Mouse IL-4-UNLB
Reactivity
Mouse
Applications
ELISA; Flow Cytometry; Immunofluorescence; Western Blot
Pricing
$295/0.5 mg | $1,185/5x0.5 mg
Rat IgG2b Isotype Control, Monoclonal Isotype Control (Cat# AAA679103)
Full Name
Rat IgG2b-BIOT
Applications
ELISA,FLISA, Flow Cytometry
Pricing
$365/0.5 mg | $1,495/5x0.5 mg
Rat IgG2b-PE/TXRD, Monoclonal Secondary Antibody (Cat# AAA675061)
Full Name
Rat IgG2b-PE/TXRD
Applications
Flow Cytometry, ELISA
Pricing
$310/0.1 mg | $1,240/5x0.1 mg
