$370/7 mL (RTU) | $350/0.1 mL (Concentrate) | $565/0.5 mL (Concentrate) | $855/1 mL (Concentrate) | $455/0.2 mL (Concentrate) | $525/15 mL (RTU) | $195/1 mL (RTU) | $205/0.04 mL (Concentrate) | $3,575/5x1 mL (Concentrate)
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Western Blot (WB) (Western Blot analysis of PTPN7 expression in transfected 293T cell line using MBS6007765: Lane 1: PTPN7 transfected lysate (40.5kD). Lane 2: Non-transfected lysate.)
Immunoprecipitation (IP) (Immunoprecipitation of PTPN7 transfected lysate using MBS6007765cand Protein A Magnetic Bead, and immunoblotted with PTPN7 mouse polyclonal antibody.)
Testing Data (Published customer image: Chronic effects of celastrol on microgliosis in Tg PS1/APPsw mice. A) Representative photomicrographs (taken with a 20x and 60x objective providing a respective magnification of 200x and 600x respectively) depicting the presence of CD45 reactive microglia around Abeta deposits in Tg PS1/APPsw treated with a placebo and celastrol. B) Histogram representing the burden of activated microglia (CD45 positive) in the cortex of Tg PS1/APPsw mice treated with placebo and celastrol pellets. Statistically significant difference in microgliosis burden (P < 0.03) was observed between placebo and celastrol treated mice. (* P < 0.05)..)
Testing Data (Published customer image: Sensitivity of cytokine detection using flow cytometry. Histograms and dot plots of IL-1beta (A) and TNF-a (B) expression in LPS-activated peritoneal macrophages versus macrophages/granulocytes isolated from cortex 24 hours after pMCAO. Light colored histograms represent cells stained with isotype control antibodies and filled histograms represent cells stained with antibodies for either IL-1beta (A) or TNF-a (B).From: Clausen et al. Journal of Neuroinflammation 2008 5:46)
Testing Data (Published customer image: Increased CD45+ microglial cells in hippocampus and cortex of the TgAPPsw mice with reduced GRK5. Representative IF results with anti-CD45 staining (green) in hippocampus (A-D) and cortex (F-I) of WT (A &F), heterozygote GRK5KO (B &G), TgAPPsw (C &H), and the double mice (D &I), respectively. Scale bar in panel I is for panels A-D and F-I: 100 um. Panels E &J, examples of high magnification views of CD45+-microglial cells in hippocampus (E) and cortex (J) of the double mice that show details of activated microglial morphology. Scale bar in panel J is for panels E &J: 20 um. Blue indicates reference DAPI staining of nuclei.From: Li et al. Journal of Neuroinflammation 2008 5:24.)
Testing Data (Published customer image: Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen et al. Journal of Neuroinflammation 2008 5:46.)
Testing Data (Published customer image Percentage of microgliosis (mean +/- s.e.m) by area in (a) Tg APPsw/CD40 def. mice versus Tg APPsw mice and in (b) Tg PSAPP/CD40 def. mice versus Tg PSAPP mice at 22 to 24 months of age calculated by quantitative image analysis. Post hoc comparison between groups are indicated by the marked bars (* p < 0.05; ** p < 0.01). Representative photographs of brain area in (c) Tg APPsw, (e) Tg APPsw/CD40 def., (d) Tg PSAPP and (f) Tg PSAPP/CD40 def. mice stained with CD45 antibody (each bar represents 0.1 mm).Laporte et al..)
Testing Data (Published customer image: Inflammatory response following permanent MCA occlusion. (A-C) Dot plots of viable CD11b+CD45high macrophages/granulocytes (top right quadrants) and CD11b+CD45dim microglia (bottom right quadrants) in cortex from unmanipulated control mice (A, B), and mice exposed to pMCAO with 24 hour survival (C). (D) At 24 hours, flow cytometric analysis of the CD11b+CD45high profiles showed that approximately half of the population consisted of CD45highGr1+ granulocytes. (E) Quantification of CD11b+CD45dim and CD11b+CD45high cells in unmanipulated control mice (n = 10), in mice 6 (n = 7), 12 (n = 7), or 24 hours after pMCAO (n = 10), and in sham-operated mice 24 hours after pMCAO (n = 7). (F) Bar graphs showing equal recruitment of CD11b+CD45highGr1- macrophages and CD11b-CD45highGr1+ granulocytes in unmanipulated mice, in mice 6, 12, or 24 hours after pMCAO, and in sham-operated mice 24 hours after pMCAO. (G, H) Bar graphs showing the mean fluorescent intensity (MFI) of CD45 expression by CD45dim microglia (G) and CD45high macrophages/granulocytes (H). *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen et al. Journal of Neuroinflammation 2008 5:46.)
Testing Data (Published customer image: Colocalization of activated microglial cells with Abeta plaques and IL-1beta immunoreactivity in the TgAPPsw mice with reduced GRK5. Panels A-C, IF staining of Abeta+ plaques (A, red) and surrounding CD45+ microglial cells (B, green), as well as their merged view (C) in the double mice. Scale bar: 50 um for panels A-C. Panels D-F, IF staining of Abeta+ plaques (D, red) and surrounding CD11b+ microglial cells (E, green), as well as their merged view (F) in the double mice. Scale bar: 50 um for panels D-F. Panel G, an example of merged view for CD45 (green) and CD11b/c (clone OX42, red) co-staining of the microglial cells. The image showed that, at least in this particular experimental paradigm, the CD45 antibody stained more specifically for the microglial cell profiles; while the OX42 antibody, in addition to its positive staining of the microglial cells, also non-specifically decorated the plaques. Scale bar: 45 um. Panel H, an example of merged view for Abeta+ plaques (red) and surrounding IL-1beta immunoreactivity (green) in the double mice. Scale bar: 30 um. Panel I, colocalization of CD45+ microglial cells (green) with IL-1beta immunoreactivity (red) in the double mice. Scale bar: 30 um. Blue indicates reference DAPI staining of nuclei.From: Li et al. Journal of Neuroinflammation 2008 5:24.)
Testing Data (Published customer image:e) Cryosectioned brain samples from day 6 p.i. were stained for WNV envelope protein (green), CD45 (as a pan-leukocyte marker; red), and DAPI (blue) and utilized for laser scanning confocal microscopy (20X images are shown and are representative of n = 5 mice per group).From: Wang P, Bai F, Zenewicz LA, Dai J, Gate D, et al. (2012) IL-22 Signaling Contributes to West Nile Encephalitis Pathogenesis. PLoS ONE 7(8): e44153.)
Testing Data (Published customer image: Cytokine expression in segregated populations of cells following stroke. (A, B) Dot plots showing CD11b+CD45high macrophages/granulocytes (upper right quadrants) and CD11b+CD45dim microglia (bottom right quadrants) expressing IL-1beta (A) or TNF-a (B). (C-J) Bar graphs showing numbers and proportions of IL-1beta (C, D), TNF-a (F, G) and IL-1beta/TNF-a co-expressing (I, J) CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in unmanipulated control mice (n = 10), in mice 6 (n = 7), 12 (n = 7), or 24 hours after pMCAO (n = 10), and in sham-operated mice 24 hours after pMCAO (n = 7). (E, H) Comparison of the MFI values for IL-1beta (E) and TNF-a (H) in viable CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in unmanipulated mice, in mice 6, 12, or 24 hours after pMCAO, and in sham-operated mice 24 hours after pMCAO. Macrophages/granulocytes express significantly more IL-1beta than do microglial in unmanipulated mice, in mice 6, 12, or 24 hours after pMCAO, and in sham-operated mice 24 hours after pMCAO (E), whereas microglial cells express significantly higher levels of TNF-a than do macrophages/granulocytes at 12 h and 24 hours, and in sham-operated mice 24 hours after pMCAO (H). (K) CD11b+CD45highGr1- macrophages and not CD11b+CD45highGr1+ granulocytes are the main producers of IL-1beta and TNF-a 24 hours after pMCAO. *P < 0.05, **P < 0.01, and ***P < 0.001.From: http://www.jneuroinflammation.com/content/5/1/46.)
Western Blot (WB) (Western BlotPositive WB detected in: Rat heart tisue,Rat kidney tissueAll lanes: PTPMT1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23,16,17 KDaObserved band size: 23 KDa)
Immunohistochemistry (IHC) (IHC image of MBS1494066 diluted at 1:1200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS1491825 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: Tyrosine-protein phosphatase non-receptor type 5 antibody at 12ug/ml+ HepG-2 whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 64,61 kDaObserved band size: 64 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human spleen tissue using MBS7005115 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue using MBS7005115 at dilution 1:100)
Western Blot (WB) (Western blotAll lanes: PTPN6 antibody at 12ug/mlLane 1: Jurkat whole cell lysateLane 2: Raji whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 68,64,71 kDaObserved band size: 68 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS1489594 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human spleen tissue using MBS1489594 at dilution 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of MCF-7 cells using MBS1489594 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of Focal Adhesion Kinase (pY397) expression in HEK293T (A), MDA-MB-231 (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of Focal Adhesion Kinase (pY397) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Typical Testing Data/Standard Curve
Testing Data
Western Blot (WB) (Western blot of rat hippocampal lysate showing specific immuno labeling of the ~180 kDa NR2A subunit of the NMDAR phosphorylated at Tyr1325 in the first lane (-). Phospho specificity is shown a in the second lane (+) where immuno labeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).)
Western Blot (WB) (Western blot analysis of PTP kappa expression in HEK293T (A), Raw264.7 (B), PC12 (C) whole cell lysates.)
Western Blot (WB) (Western blotAll lanes: RMDN3 antibody at 4ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateLane 3:mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 53,39 kDaObserved band size: 52 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skin using MBS1497052 at dilution 1:100)
Western Blot (WB) (Western blot detection of CD45 in Jurkat and MCF7 cell lysates using CD45 mouse mAb (1:2000 diluted).Predicted band size:147KDa.Observed band size:180KDa.)
Western Blot (WB) (Western blot analysis using PTPRC mAb against HEK293 (1) and PTPRC (AA)
Western Blot (WB) (Western blot analysis using PTPRC mouse mAb against Hela (1) and A431 (2) cell lysate.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PTPRC mouse mAb with DAB staining.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RMDN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB) (Western blotAll lanes: Signal-regulatory protein delta antibody at 14ug/mlLane 1: 293T whole cell lysateLane 2: Mouse spleen tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 22 kDaObserved band size: 22 kDa)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RO Monoclonal Antibody (SPM125).)
Immunohistochemistry (IHC) (Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC) (Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB) (Cell lysates of Jurkat(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD45 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
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