ACE2 recombinant protein

ACE2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8118589

• Gene Names: ACE2; ACEH
• Purity: >95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC.
• Synonyms: ACE2; ACE2 Protein; Human; Recombinant (His Tag); Human ACE2/Angiotensin-Converting Enzyme 2 Protein (His Tag) (HPLC-verified); angiotensin I converting enzyme 2; ACEH Protein; ACE2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 734aa
• Sequence: Met1-Ser740

• Species: Human
• Tag: C-His
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Gln18
• Protein Construction: |A DNA sequence encoding the human ACE2 (NP_068576.1) (Met1-Ser740) was expressed with a polyhistidine tag at the C-terminus
• Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized 2019-nCoV Spike Protein (RBD, mFc Tag) at 2ug/mL (100uL/well) can bind human ACE2 protein (His tag). The EC50 of ACE2 is 10-30ng/mL.; 2. Measured by its ability to cleave a fluorogenic peptide substrate, McaYVADAPK (Dnp) OH, R&D Systems; The specific activity is >1000 pmols/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized 2019-nCoV Spike Protein (RBD, mFc Tag)(40592-V05H) at 2ug/mL (100uL/well) can bind human ACE2 protein (His tag),the EC50 of ACE2 is 20-90ng/mL.)

Testing Data

Related Product Information for ACE2 recombinant protein

Angiotensin-converting enzyme 2 (ACE2), a first homolog of ACE, regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular, renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme, an homolog of ACE, hydrolyzes angiotensin (Ang) I to produce Ang-(1-9), which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus, the major biologically active product of ACE2 is Ang-(1-7), which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension, cardiac function, heart function and diabetes, and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS), ACE, AngII, and AT1R promote the disease pathogenesis, whereas ACE2 and the AT2R protect from ARDS. Importantly, ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore, the recent explosion of research into the ACE2 homolog, collectrin, has revealed a new physiological function of ACE2 as an amino acid transporter, which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis.

References

Koitka A, et al. (2008) Angiotensin converting enzyme 2 in the kidney. Clin Exp Pharmacol Physiol. 35(4): 420-5.Raizada MK, et al. (2007) ACE2: a new target for cardiovascular disease therapeutics. J Cardiovasc Pharmacol. 50(2): 112-9.Imai Y, et al. (2007) Angiotensin-converting enzyme 2 (ACE2) in disease pathogenesis. Circ J. 74(3): 405-10.Turner AJ, et al. (2004) ACE2: from vasopeptidase to SARS virus receptor. Trends Pharmacol Sci. 25(6): 291-4.

NCBI and Uniprot Product Information

• NCBI GI #: 11225609
• NCBI GeneID: 59272
• NCBI Accession #: NP_068576.1
• NCBI GenBank Nucleotide #: NM_021804.2
• UniProt Accession #: Q9BYF1; Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6; C7ECU1
• Molecular Weight: 92,463 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: ACE2
• NCBI Official Synonym Symbols: ACEH
• NCBI Protein Information: angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• Protein Family: Angiotensin-converting enzyme
• UniProt Gene Name: ACE2
• UniProt Synonym Gene Names: ACEH
• UniProt Entry Name: ACE2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

Uniprot Description

ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.23; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; plasma membrane; extracellular region; lipid raft

Molecular Function: peptidyl-dipeptidase activity; viral receptor activity; protein binding; metallopeptidase activity; carboxypeptidase activity; zinc ion binding; peptide hormone binding; endopeptidase activity; glycoprotein binding

Biological Process: virion attachment, binding of host cell surface receptor; entry of virus into host cell; maternal process involved in pregnancy; regulation of vasodilation; proteolysis; angiotensin maturation; regulation of systemic arterial blood pressure by renin-angiotensin; regulation of cell proliferation; regulation of cytokine production; cellular protein metabolic process; regulation of vasoconstriction; regulation of inflammatory response; angiotensin catabolic process in blood; receptor biosynthetic process; angiotensin mediated drinking behavior