Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit | DUSP1 elisa kit

Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit

Cat. Num. AAA453652

• Gene Names: DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
• Reactivity: Human
• Synonyms: Dual Specificity Phosphatase 1 (DUSP1); Human Dual Specificity Phosphatase 1 (DUSP1) ELISA Kit; CL100; HVH1; MKP1; PTPN10; Mitogen-activated protein kinase phosphatase 1; Protein-tyrosine phosphatase CL100; Dual specificity protein phosphatase hVH1; DUSP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DUSP1. No significant cross-reactivity or interference between DUSP1 and analogues was observed.
• Sequence Length: 367

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 12.9pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DUSP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DUSP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DUSP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DUSP1 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DUSP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DUSP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DUSP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DUSP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758204
• NCBI GeneID: 1843
• NCBI Accession #: NP_004408.1
• NCBI GenBank Nucleotide #: NM_004417.3
• UniProt Accession #: P28562; Q2V508; D3DQL9
• Molecular Weight: 39,298 Da
• NCBI Official Full Name: dual specificity protein phosphatase 1
• NCBI Official Synonym Full Names: dual specificity phosphatase 1
• NCBI Official Symbol: DUSP1
• NCBI Official Synonym Symbols: HVH1; MKP1; CL100; MKP-1; PTPN10
• NCBI Protein Information: dual specificity protein phosphatase 1
• UniProt Protein Name: Dual specificity protein phosphatase 1
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP1
• UniProt Synonym Gene Names: CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1

NCBI Description

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.