Typical Testing Data/Standard Curve (for reference only)
Mouse AATK ELISA Kit | AATK elisa kit
Mouse AATK (Apoptosis Associated Tyrosine Kinase) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AATK. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AATK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AATK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AATK. You can calculate the concentration of AATK in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene contains a tyrosine kinase domain at the N-terminus and a proline-rich domain at the C-terminus. This gene is induced during apoptosis, and expression of this gene may be a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells. This gene has been shown to produce neuronal differentiation in a neuroblastoma cell line. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
Uniprot Description
Lmr1: a protein kinase kinase of the Lmr family. Predominantly expressed in the nervous system and is involved in neurite extension and apoptosis of cerebellar granule cells. Contains a tyrosine kinase domain at the N-terminal end and a proline-rich domain at the C-terminal end. May be necessary for the induction of growth arrest and/or apoptosis of myeloid cells induced by cytokine withdrawal, such as IL3, and during G-CSF-induced terminal differentiation of myeloblasts to granulocytes. Four alternatively spliced isoforms have been reported.
Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.11.1; TK group; Lmr family
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: recycling endosome; perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; ATP binding
Biological Process: neuron apoptosis; negative regulation of axon extension; brain development; Rab protein signal transduction
Research Articles on AATK
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Product Notes
The Mouse AATK aatk (Catalog #AAA2504462) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504462 ELISA Kit recognizes Mouse AATK. It is sometimes possible for the material contained within the vial of "AATK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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