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914 results for "nuclear-receptor-binding-factor" - showing 1-50
a-Fetoprotein Receptor, Monoclonal Antibody (Cat# AAA602268)
Full Name
a-Fetoprotein Receptor (AFPr)
Gene Names
NR5A2; B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta
Reactivity
Human
Applications
WB, ICC
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$915/1 mg | $4,110/5x1 mg
Estrogen Receptor Alpha, Monoclonal Antibody (Cat# AAA190147)
Full Name
Estrogen Receptor- alpha Monoclonal Antibody
Gene Names
ESR1; ER; ESR; Era; ESRA; NR3A1; DKFZp686N23123
Applications
Immunoblot, Immunoprecipitation
Pricing
$480/0.1 mg | $2,115/5x0.1 mg
Western Blot (WB)
(Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
EGF Receptor, Monoclonal Antibody (Cat# AAA475155)
Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Monkey
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot detection of EGFR in A549 and COS7 cell lysates using EGFR mouse mAb (dilution 1:2000).Predicted band size:134 Kda.Observed band size:175KDa.)
EGF Receptor, Monoclonal Antibody (Cat# AAA475211)
Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Monkey
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Steroidogenic Factor 1, ELISA Kit (Cat# AAA46090)
Full Name
Mouse Steroidogenic Factor 1 ELISA Kit
Gene Names
NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
Reactivity
Mouse
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate,PC3 whole cell lysate,HepG2 whole cell lysate,Mouse kidney tissue,Mouse brain tissueAll lanes: NR5A2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 62,57,43,54 KDaObserved band size: 62,54 KDa)
NR5A2, Polyclonal Antibody (Cat# AAA7048770)
Full Name
NR5A2 Antibody
Gene Names
NR5A2; B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Activity Data
(Figure. The binding activity of RANKL with PPARg.Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a protein that in humans is encoded by the TNFSF11 gene. RANKL is a member of the tumor necrosis factor (TNF) cytokine family, it binds to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. It may also bind to osteoprotegerin, a protein secreted mainly by cells of the osteoblast lineage which is a potent inhibitor of osteoclast formation by preventing binding of RANKL to RANK. RANKL also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. Besides, Peroxisome Proliferator Activated Receptor Gamma (PPARg) has been identified as an interactor of RANKL, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse RANKL and recombinant mouse PPARg. Briefly, RANKL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PPARg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-RANKL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of RANKLand PPARg was shown in Figure 1, and this effect was in a dose dependent manner.)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Active Protein (Cat# AAA2105122)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$225/0.01 mg | $490/0.05 mg | $755/0.1 mg | $940/0.2 mg | $1,830/0.5 mg | $2,710/1 mg | $6,620/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
PDGFRB, Polyclonal Antibody (Cat# AAA9215252)
Full Name
PDGFRB Antibody (N-term)
Gene Names
PDGFRB; IMF1; IBGC4; JTK12; PDGFR; CD140B; PDGFR1; PDGFR-1
Reactivity
Human
Applications
EIA, IHC-P, IF, WB, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western BlotPositive WB detected in: Rat heart tissueAll lanes: MECR antibody at 3.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 41,33 KDaObserved band size: 41 KDa)
MECR, Polyclonal Antibody (Cat# AAA1492204)
Full Name
MECR Antibody
Gene Names
MECR; NRBF1; CGI-63; FASN2B
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with STAT3 Mouse Monoclonal Antibody (STAT3/2409).)
STAT3/Signal Transducer and Activator of Transcription 3, Monoclonal Antibody (Cat# AAA4380497)
Full Name
STAT3/Signal Transducer and Activator of Transcription 3
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blotAll lanes: NRBF2 antibody at 5.51ug/mlLane 1:HepG2 whole cell lysateLane 2:A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 33,27,32 kDaObserved band size: 32 kDa)
NRBF2, Polyclonal Antibody (Cat# AAA7045375)
Full Name
NRBF2 Antibody
Gene Names
NRBF2; COPR; COPR1; COPR2; NRBF-2
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunofluorescence (IF)
(MBS9600098 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9600098) and mouse anti-beta tubulin Ab(MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue))
FGFR3, Polyclonal Antibody (Cat# AAA9600098)
Full Name
FGFR3 Antibody
Gene Names
FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide Affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page
(SDS-PAGE Analysis Purified SREBP1 Mouse Monoclonal Antibody (SREBP1/1578). Confirmation of Integrity and Purity of Antibody.)
SREBP1, Monoclonal Antibody (Cat# AAA4381269)
Full Name
SREBP1
Gene Names
SREBF1; SREBP1; bHLHd1
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
SDS-Page
(SDS-PAGE Analysis Purified SREBP2 Mouse Monoclonal Antibody (SREBP2/1579). Confirmation of Purity and Integrity of Antibody.)
SREBP2, Monoclonal Antibody (Cat# AAA4381270)
Full Name
SREBP2
Gene Names
SREBF2; SREBP2; bHLHd2; SREBP-2
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
FMR1, Polyclonal Antibody (Cat# AAA9207834)
Full Name
FMR1 Antibody (N-term)
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, mouse
Applications
IHC-P, WB, FC,E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of NR5A1 Antibody (N-term) (MBS9205805) in HepG2 cell line lysates (35ug/lane).NR5A1 (arrow) was detected using the purified Pab.)
NR5A1, Polyclonal Antibody (Cat# AAA9205805)
Full Name
NR5A1 Antibody (N-term)
Gene Names
NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXY3
Reactivity
Human
Predicted Reactivity: Bovine, Mouse, Pig, Rat
Predicted Reactivity: Bovine, Mouse, Pig, Rat
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NLRC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NLRC4, Polyclonal Antibody (Cat# AAA9126899)
Full Name
NLRC4
Gene Names
NLRC4; CLAN; IPAF; AIFEC; CLAN1; CLANA; CLANB; CLANC; CLAND; FCAS4; CARD12; CLR2.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF)
( Immunofluorescence staining of fixed A549 cells with anti-EDAR antibody EDAR12 (Ab00785) Immunofluorescence analysis of paraformaldehyde fixed A549 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of EDAR12 (Ab00785-1.1) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing weak membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00785-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (Ab00178-1.1) followed by Alexa Fluor® 488 secondary antibody.)
EDAR, Monoclonal Recombinant Antibody (Cat# AAA488351)
Full Name
Anti-EDAR [EDAR12]
Gene Names
Edar; dl; ED3; ED5; ED1R; EDA3; EDA-A1R
Reactivity
Chicken, Dog, Rat, Human, Mouse
Applications
EIA, SPR, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot analysis of RAP1 using HeLa whole cell lysates)
RAP1, Polyclonal Antibody (Cat# AAA9608915)
Full Name
RAP1 Antibody
Gene Names
TERF2IP; RAP1; DRIP5
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CHD9 using Jurkat whole cell lysates)
CHD9, Polyclonal Antibody (Cat# AAA9609701)
Full Name
CHD9 Antibody
Gene Names
CHD9; AD013; CHD-9; CReMM; KISH2; PRIC320
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
( Immunofluorescence staining of fixed Daudi cells with anti-CD40 antibody 5D12 Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 5D12 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD40, Monoclonal Recombinant Antibody (Cat# AAA488354)
Full Name
Anti-CD40 [5D12]
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Cynomolgus, Marmoset Monkey, Human
Applications
BL, EIA, FC/FACS, SPR, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fas antibody R-125224 (Ab00802) Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (Ab00802-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fas, Monoclonal Recombinant Antibody (Cat# AAA488362)
Full Name
Anti-Fas [R-125224]
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Human
Applications
EIA, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
EGF Receptor, Monoclonal Antibody (Cat# AAA475201)
Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-EGFR antibody Matuzumab HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
EGFR, Recombinant Antibody (Cat# AAA488209)
Full Name
Anti-EGFR [Matuzumab]
Reactivity
Human
Applications
FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Western blot detection of GCNF in Jurkat, MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.)
GCNF, Monoclonal Antibody (Cat# AAA476329)
Full Name
Anti-GCNF Mouse mAb
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
Human
Applications
WB, IF
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD36 antibody (Center) in CEM cell line lysates (35ug/lane). CD36 (arrow) was detected using the purified Pab.)
CD36, Polyclonal Antibody (Cat# AAA9214096)
Full Name
Rabbit anti-Human, Mouse CD36 Polyclonal Antibody
Gene Names
CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
Reactivity
Reactivity: Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
IF, WB, IHC-P, FC, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Sample Type: Human brainBrain, cortex)
FKBP5, Polyclonal Antibody (Cat# AAA3211818)
Full Name
FKBP5 antibody - C-terminal region
Gene Names
FKBP5; P54; AIG6; FKBP51; FKBP54; PPIase; Ptg-10
Reactivity
Tested Reactivity: Human, Mouse
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
SDS-Page
(SDS-PAGE Analysis Purified HER-4/ERBB4 Mouse Monoclonal Antibody (HFR-1). Confirmation of Purity and Integrity of Antibody.)
HER-4/ERBB4, Monoclonal Antibody (Cat# AAA4380869)
Full Name
HER-4/ERBB4
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse
Predicted to work with Rat, Chicken.
Predicted to work with Rat, Chicken.
Applications
FC/FACS, IF
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Lanes:Lane1: 30 ug Human liverLane2: 30 ug Rat liverLane3: 30 ug Mouse (wild-type) liverLane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liverLane5: 30 ug Human muscleLane6: 30 ug Rat muscleLane7: 30 ug Mouse musclePrimary Antibody Dilution:1:1000Secondary Antibody:Secondary Antibody Dilution:1:10000Gene Name:PPARGC1ASubmitted by:Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Center)
PPARGC1A, Polyclonal Antibody (Cat# AAA3204399)
Full Name
PPARGC1A antibody - N-terminal region
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Activity Data
(Figure1. TRAP levels in the cell of RAW264.7 cells induced by RANKL.Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a member of the tumor necrosis factor (TNF) superfamily. RANKL has been identified to affect the immune system and control bone regeneration and remodeling. RANKL is an apoptosis regulator gene, a binding partner of osteoprotegerin (OPG), a ligand for the receptor RANK and controls cell proliferation by modifying protein levels of Id4, Id2 and cyclin D1. The protein can bind to RANK on cells of the myeloid lineage and functions as a key factor for osteoclast differentiation and activation. Thus, we use recombinant RANKL to induce RAW264.7 mouse monocyte/macrophage cells to differentiate to osteoclast cell. RAW264.7 mouse monocyte/macrophage cells were seeded into 24-well plates at a density of 2×105 and allowed to attach overnight, then treated with RANKL (1ng/mL, 10ng/mL) and incubated for 72h. Then we assay TRAP (a kind of enzyme expressed by osteoclast cell) by ELASA. Result: TRAP levels in the cell supernatant of RAW264.7 cells increased significantly after stimulated with RANK, the data was shown in Table 1 and Figure1.)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Active Protein (Cat# AAA2105123)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPGL; RANKL; Ly109l; Trance
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using TNFRSF1A antibody. Blue: DAPI for nuclear staining.)
TNFRSF1A, Polyclonal Antibody (Cat# AAA125977)
Full Name
TNFRSF1A Monoclonal Antibody
Gene Names
TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
Reactivity
Human
Applications
IF
Purity
Affinity Purification
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL
Immunohistochemistry (IHC)
(Anti-RORA / ROR Alpha antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
RORA / ROR Alpha, Polyclonal Antibody (Cat# AAA243643)
Full Name
Rabbit Polyclonal to Human RORA / ROR Alpha
Gene Names
RORA; ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
Reactivity
Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Predicted: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin
Purity
Immunoaffinity Purified
Pricing
$610/0.05 mg | $2,685/5x0.05 mg
Western Blot (WB)
(Western blot analysis of CCL2 Antibody (C-term) in Hela cell line lysates (35ug/lane). CCL2 (arrow) was detected using the purified Pab.)
CCL2, Polyclonal Antibody (Cat# AAA9207814)
Full Name
CCL2 Antibody (C-term)
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of EEF1A1 Antibody (C-term) in MCF-7 cell line lysates (35ug/lane). EEF1A1 (arrow) was detected using the purified Pab.)
EEF1A1, Polyclonal Antibody (Cat# AAA9205539)
Full Name
EEF1A1 Antibody (C-term)
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Reactivity: Human
Predicted Reactivity: Xenopus, Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken, Zebrafish
Predicted Reactivity: Xenopus, Bovine, Hamster, Horse, Mouse, Rabbit, Rat, Chicken, Zebrafish
Applications
IF, WB, IHC-P, FC
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of NR5A1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
NR5A1, Polyclonal Antibody (Cat# AAA9603921)
Full Name
NR5A1 Antibody
Gene Names
NR5A1; ELP; SF1; FTZ1; POF7; SF-1; AD4BP; FTZF1; SPGF8; SRXX4; SRXY3; hSF-1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CENPR expression in A-431 cell lysate at 25ug/lane, The lane on the left is treated with the antigen-specific peptide.)
CENPR, Polyclonal Antibody (Cat# AAA9603848)
Full Name
CENPR Antibody
Gene Names
ITGB3BP; CENPR; NRIF3; TAP20; CENP-R; HSU37139
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9603005 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
NRBP1, Polyclonal Antibody (Cat# AAA9603005)
Full Name
NRBP1 Antibody
Gene Names
NRBP1; MADM; NRBP; BCON3; MUDPNP
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data
(Figure. The binding activity of TNFRSF11A with TRAF5.RANK tumor necrosis factor receptor superfamily member 11A protein (TNFRSF11A) also known as receptor Activator of Nuclear Factor kappa B (RANK) or TRANCE Receptoris a member of the tumor necrosis factor receptor (TNFR) molecular sub-family. TNFRSF11A is the receptor for RANK-Ligand (RANKL) and part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is associated with bone remodeling and repair, immune cell function, lymph node development, thermal regulation, and mammary gland development. Besides, TNF Receptor Associated Factor 5 (TRAF5) has been identified as an interactor of TNFRSF11A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF11A and recombinant human TRAF5. Briefly, TNFRSF11A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TRAF5-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF11A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF11A and TRAF5 was shown in Figure 1, and this effect was in a dose dependent manner.)
Receptor Activator Of Nuclear Factor Kappa B (RANk), Active Protein (Cat# AAA2105163)
Full Name
Active Receptor Activator Of Nuclear Factor Kappa B (RANk)
Gene Names
TNFRSF11A; FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$225/0.01 mg | $480/0.05 mg | $740/0.1 mg | $915/0.2 mg | $1,790/0.5 mg | $2,645/1 mg | $6,465/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-4/ERBB4 Mouse Monoclonal Antibody (ERBB4/2582).)
HER-4/ERBB4, Monoclonal Antibody (Cat# AAA4380550)
Full Name
HER-4/ERBB4
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TRAF3 antibody.)
TRAF3, Polyclonal Antibody (Cat# AAA9413819)
Full Name
TRAF3 antibody
Gene Names
TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL
Western Blot (WB)
(Western blot analysis of HeLA cell lysate, using C1QBP Antibody. The lane on the left is treated with the antigen-specific peptide.)
C1QBP, Polyclonal Antibody (Cat# AAA9607331)
Full Name
C1QBP Antibody
Gene Names
C1QBP; p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R; COXPD33; SF2AP32
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of TAAR5 using HepG2 whole cell lysates)
TAAR5, Polyclonal Antibody (Cat# AAA9609041)
Full Name
TAAR5 Antibody
Gene Names
TAAR5; PNR
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis NR5A2 using COS7 whole cell lysates)
NR5A2, Polyclonal Antibody (Cat# AAA9608815)
Full Name
NR5A2 Antibody
Gene Names
NR5A2; B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(Sample Type :Mouse retina Primary Antibody Dilution :1:200 Secondary Antibody :Donkey anti rabbit-Cy3Secondary Antibody Dilution :1:1000 Color/Signal Descriptions :Red: PPARGC1AGene Name :PPARGC1ASubmitted by :Dr. Joshua Sanes )
PPARGC1A, Polyclonal Antibody (Cat# AAA3200467)
Full Name
PPARGC1A antibody - N-terminal region
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IF, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ITGB3BP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
ITGB3BP, Polyclonal Antibody (Cat# AAA9125664)
Full Name
ITGB3BP Polyclonal Antibody
Gene Names
ITGB3BP; CENPR; NRIF3; TAP20; CENP-R; HSU37139
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC)
(RORA antibody - middle region Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Nucleus in Human Pineal TissuePrimary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec)
RORA, Polyclonal Antibody (Cat# AAA3207815)
Full Name
RORA antibody - middle region
Gene Names
RORA; ROR1; ROR2; ROR3; RZRA; NR1F1; IDDECA; RZR-ALPHA
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
WB, IHC
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse liver, using NR5A2 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
NR5A2, Polyclonal Antibody (Cat# AAA9133716)
Full Name
NR5A2 Polyclonal Antibody
Gene Names
NR5A2; B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta
Reactivity
Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of NRIP1 expression in A498 (Human Kidney Carcinoma) Whole Cell Lysate ;, The lane on the left is treated with the antigen-specific peptide.)
NRIP1, Polyclonal Antibody (Cat# AAA9604154)
Full Name
NRIP1 Antibody
Gene Names
NRIP1; RIP140
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF)
(MBS9605711 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NRBF2, Polyclonal Antibody (Cat# AAA9605711)
Full Name
NRBF2 Antibody
Gene Names
NRBF2; COPR; COPR1; COPR2; NRBF-2
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GAB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 80s.)
GAB1, Polyclonal Antibody (Cat# AAA9125030)
Full Name
GAB1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
