Rabbit RORA / ROR Alpha Polyclonal Antibody | anti-RORA antibody

Rabbit Polyclonal to Human RORA / ROR Alpha

Cat. Num. AAA243643

• Gene Names: RORA; ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
• Reactivity: Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity); Predicted: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: RORA / ROR Alpha; Polyclonal Antibody; Rabbit Polyclonal to Human RORA / ROR Alpha; Anti-RORA / ROR Alpha Antibody (Ligand-binding Domain) IHC-plus; RORA; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Roralpha; Transcription factor RZR-alpha; RAR-related orphan receptor A; ROR-alpha; ROR3; RZRA; NR1F1; ROR Alpha; RZR-ALPHA; Human RORA; anti-RORA antibody

• Host: Rabbit
• Reactivity: Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity); Predicted: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ROR Alpha. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 548

• Applicable Applications for anti-RORA antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3 ug/ml)
• Immunogen Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from ligand-binding domain of human ROR Alpha.
• Antigen Type: Synthetic peptide
• Immunogen: RORA / ROR Alpha antibody was raised against synthetic 20 amino acid peptide from ligand-binding domain of human ROR Alpha. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%); Turkey, Chicken, Xenopus (95%); Stickleback, Pufferfish, Zebrafish (90%).
• Antigen Modification: Ligand-binding Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-RORA / ROR Alpha antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-RORA / ROR Alpha antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-ROR Alpha antibody IHC of human brain, spinal trigeminal nucleus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-RORA antibody

Retinoid-related orphan receptor alpha (ROR alpha), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect the development of the central nervous system. Deletion of ROR alpha in mice causes severe impairment in the differentiation of cerebellar Purkinje neurons and results in the 'staggerer' phenotype. ROR alpha has been suggested as a potential target in the treatment of chronic inflammatory diseases, including atherosclerosis and rheumatoid arthritis.

Product Categories/Family for anti-RORA antibody

Family: NHR; Subfamily: NR1 Thyroid hormone-like

NCBI and Uniprot Product Information

• NCBI GI #: 4506577
• NCBI GeneID: 6095
• NCBI Accession #: NP_002934.1
• NCBI GenBank Nucleotide #: NM_002943.3
• UniProt Accession #: P35398; P35397; P35399; P45445; Q495X4; Q96H83
• Molecular Weight: 53,556 Da
• NCBI Official Full Name: nuclear receptor ROR-alpha isoform c
• NCBI Official Synonym Full Names: RAR-related orphan receptor A
• NCBI Official Symbol: RORA
• NCBI Official Synonym Symbols: ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
• NCBI Protein Information: nuclear receptor ROR-alpha; nuclear receptor RZR-alpha; transcription factor RZR-alpha; retinoid-related orphan receptor alpha; nuclear receptor subfamily 1 group F member 1; thyroid hormone nuclear receptor alpha variant 4; retinoic acid receptor-related orphan receptor alpha
• UniProt Protein Name: Nuclear receptor ROR-alpha
• Protein Family: Nuclear receptor
• UniProt Gene Name: RORA
• UniProt Synonym Gene Names: NR1F1; RZRA
• UniProt Entry Name: RORA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

RORA iso1: Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. Monomer. Interacts (via the DNA-binding domain) with HIF1; the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. By hypoxia and melatonin. Widely expressed in a number of tissues. Activated by CaMK4. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; beta-catenin binding; oxysterol binding; steroid hormone receptor activity; transcription factor binding; transcription factor activity

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; granule cell precursor proliferation; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; regulation of macrophage activation; positive regulation of circadian rhythm; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; muscle cell differentiation; regulation of transcription, DNA-dependent; negative regulation of inflammatory response; xenobiotic metabolic process; cerebellar Purkinje cell differentiation; regulation of steroid metabolic process; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; angiogenesis; nitric oxide biosynthetic process; cGMP metabolic process; regulation of smoothened signaling pathway