Western Blot (WB)
(Western blot detection of GCNF in Jurkat, MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.)
Mouse anti-Human GCNF Monoclonal Antibody | anti-GCNF antibody
Anti-GCNF Mouse mAb
Western Blot (WB)
(Western blot detection of GCNF in Jurkat, MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.)
Western Blot (WB)
(Western blot detection of GCNF in Jurkat, MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-GCNF mouse mAb (dilution 1:200).)
Immunocytochemistry (ICC)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-GCNF mouse mAb (dilution 1:200).)
Western Blot (WB)
(Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.)
Western Blot (WB)
(Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue)
Immunofluorescence (IF)
(Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.)
UniPort Summary Function: Orphan nuclear receptor. Binds to a response element containing the sequence 5-TCAAGGTCA-3. May be involved in the regulation of gene expression in germ cell development during gametogenesis (By similarity).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]
Uniprot Description
NR6A1: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. Homodimer. Interacts with UIMC1. Shows highest expression in the germ cells of the adult testis. Belongs to the nuclear hormone receptor family. NR6 subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Nuclear receptor
Chromosomal Location of Human Ortholog: 9q33.3
Cellular Component: nucleoplasm; nucleus; transcription factor complex
Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; sequence-specific DNA binding
Biological Process: cell proliferation; gamete generation; negative regulation of transcription from RNA polymerase II promoter; spermatogenesis; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter
Research Articles on GCNF
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Product Notes
The GCNF nr6a1 (Catalog #AAA476329) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-GCNF Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GCNF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Researchers should empirically determine the suitability of the GCNF nr6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCNF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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