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Type
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Gene Name
Isotype
Host
Application
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5204 results for "gtpase-activating-protein" - showing 1-50
Breakpoint cluster region protein (BCR), Recombinant Protein (Cat# AAA718453)
Full Name
Recombinant Human Breakpoint cluster region protein (BCR) , partial
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Western Blot (WB)
(All lanes: Carbonic anhydrase 1 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:293T whole cell lysateLane 3:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 53,14 kDaObserved band size: 53 kDa)
Ras GTPase-activating protein-binding protein 1, Polyclonal Antibody (Cat# AAA1499184)
Full Name
Rabbit anti-human Ras GTPase-activating protein-binding protein 1 polyclonal Antibody(G3BP1)
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Western Blot (WB)
(All lanes: ADP-ribosylation factor GTPase-activating protein 1 antibody at 8ug/mlLane 1:PC-3 whole cell lysateLane 2:Hela whole cell lysateLane 3:mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45,46,32,40 kDaObserved band size: 45 kDa)
ADP-ribosylation factor GTPase-activating protein 1, Polyclonal Antibody (Cat# AAA1493690)
Full Name
Rabbit anti-human ADP-ribosylation factor GTPase-activating protein 1 polyclonal Antibody(ARFGAP1)
Gene Names
ARFGAP1; ARF1GAP; HRIHFB2281
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
TBC1 domain family, member 15, ELISA Kit (Cat# AAA9319961)
Full Name
Human TBC1 domain family member 15, TBC1D15 ELISA Kit
Gene Names
TBC1D15; RAB7-GAP
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Immunoprecipitation (IP)
(Immunoprecipitation of DLC1 transfected lysate using DLC1 rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with DLC1 mouse polyclonal antibody.)
DLC-1, Polyclonal Antibody (Cat# AAA6007450)
Full Name
DLC-1 (Rho GTPase-activating Protein 7, Deleted in Liver Cancer 1 Protein, HP Protein, Rho-type GTPase-activating Protein 7, START Domain-containing Protein 12, StARD12, StAR-related Lipid Transfer Protein 12, DLC1, ARHGAP7, KIAA1723, STARD12)
Gene Names
DLEC1; F56; DLC1
Reactivity
Human
Applications
IP
Purity
Serum
Serum
Serum
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Western Blot (WB)
(Western blotAll lanes: NDST1 antibody at 6ug/ml+ MCF7 whole cell lysateGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 101,63kDaObserved band size: 85 kDa)
Regulator of G-protein signaling 20, Polyclonal Antibody (Cat# AAA1497892)
Full Name
Rabbit anti-human Regulator of G-protein signaling 20 polyclonal Antibody
Gene Names
RGS20; RGSZ1; ZGAP1; gz-GAP; g(z)GAP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Ran GTPase activating protein 1, ELISA Kit (Cat# AAA9321016)
Full Name
Human Ran GTPase-activating protein 1, RANGAP1 ELISA Kit
Gene Names
RANGAP1; SD; Fug1; RANGAP
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
RasGAP, ELISA Kit (Cat# AAA2507347)
Full Name
Mouse RasGAP (Ras GTPase Activating Protein) ELISA Kit
Gene Names
RASA1; GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
Reactivity
Mouse
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western blot analysis of ARHGAP22 expression in HeLa (A), mouse kidney (B), rat kidney (C) whole cell lysates.)
ARHGAP22, Polyclonal Antibody (Cat# AAA821095)
Full Name
Anti-ARHGAP22 Antibody
Gene Names
ARHGAP22; RhoGAP2; RhoGap22
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7043615 at dilution of 1:100)
ARHGAP10, Polyclonal Antibody (Cat# AAA7043615)
Full Name
ARHGAP10 Antibody
Gene Names
ARHGAP10; GRAF2; PSGAP; PS-GAP
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
N-chimaerin, CHN1, ELISA Kit (Cat# AAA2602677)
Full Name
Mouse N-chimaerin, CHN1 ELISA Kit
Gene Names
Chn1; ARHGAP2; AI413815; 0610007I19Rik; 0710001E19Rik; 1700112L09Rik; 2900046J01Rik
Reactivity
Mouse
Pricing
$285/48-Strip-Wells | $440/96-Strip-Wells | $1,790/5x96-Strip-Wells | $3,280/10x96-Strip-Wells
Western Blot (WB)
(Western blotAll lanes: ARFGAP3 antibody at 4ug/mlLane 1:HepG2 whole cell lysateLane 2:Jurkat whole cell lysateLane 3:A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 57,52 kDaObserved band size: 57 kDa)
ARFGAP3, Polyclonal Antibody (Cat# AAA1493385)
Full Name
ARFGAP3 Antibody
Gene Names
ARFGAP3; ARFGAP1
Reactivity
Human
Applications
EIA, WB, IHC, IP
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7005010 at dilution of 1:100)
ARL6IP5, Polyclonal Antibody (Cat# AAA7005010)
Full Name
ARL6IP5 Antibody
Gene Names
ARL6IP5; JWA; jmx; hp22; PRAF3; Yip6b; DERP11; HSPC127; addicsin; GTRAP3-18
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%,Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: CHN1 antibody at 6.08ug/mlLane 1: 293T whole cell lysateLane 2: MCF7 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Hela whole cell lysateGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 54,39,51 kDaObserved band size: 53 kDa)
CHN1, Polyclonal Antibody (Cat# AAA7044769)
Full Name
CHN1 Antibody
Gene Names
CHN1; NC; CHN; DURS2; ARHGAP2; RHOGAP2
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)
GAP, ELISA Kit (Cat# AAA2510006)
Full Name
Human GAP (GTPase Activating Protein) ELISA Kit
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded MBS7043990 using human liver cancer at dilution 1:100)
RABGAP1L, Polyclonal Antibody (Cat# AAA7043990)
Full Name
RABGAP1L Antibody
Gene Names
RABGAP1L; HHL; TBC1D18
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
GAP, ELISA Kit (Cat# AAA2511133)
Full Name
Rat GAP (Ras Gtpase Activating Protein) ELISA Kit
Reactivity
Rat
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7045653 at dilution of 1:100)
ARHGAP42, Polyclonal Antibody (Cat# AAA7045653)
Full Name
ARHGAP42 Antibody
Gene Names
ARHGAP42; GRAF3
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blotAll lanes: RACGAP1 antibody at 2.68 ug/ml+Raji whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 71 kDaObserved band size: 71,40 kDa)
RACGAP1, Polyclonal Antibody (Cat# AAA7046264)
Full Name
RACGAP1 Antibody
Gene Names
RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human thymus tissue using MBS7051400 at dilution of 1:100)
RACGAP1, Polyclonal Antibody (Cat# AAA7051400)
Full Name
RACGAP1 Antibody
Gene Names
RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7045387 at dilution 1:100)
RANBP1, Polyclonal Antibody (Cat# AAA7045387)
Full Name
RANBP1 Antibody
Gene Names
RANBP1; HTF9A
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
GIT2, cDNA Clone (Cat# AAA1270251)
Full Name
GIT2 cDNA Clone
Gene Names
GIT2; PKL; CAT2; CAT-2
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB)
(Western blotAll lanes: RGS20 antibody at 8ug/ml+mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 44,32,22,18,26,28 kDaObserved band size: 44 kDa)
RGS20, Polyclonal Antibody (Cat# AAA1492621)
Full Name
RGS20 Antibody
Gene Names
RGS20; RGSZ1; ZGAP1; gz-GAP; g(z)GAP
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
ARHGAP29, cDNA Clone (Cat# AAA1274934)
Full Name
ARHGAP29 cDNA Clone
Gene Names
ARHGAP29; PARG1
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB)
(Western BlotPositive WB detected in:Mouse heart tissueAll lanes: SRGAP2B antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54 kDaObserved band size: 54 kDa)
SRGAP2B, Polyclonal Antibody (Cat# AAA7049751)
Full Name
SRGAP2B Antibody
Gene Names
SRGAP2B; SRGAP2L; SRGAP2P2
Reactivity
Human, Mouse
Applications
EIA, WB, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in:Hela whole cell lysate,Mouse brain tissueAll lanes: RASA1 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 117,101,59,102 kDaObserved band size: 117 kDa)
RASA1, Polyclonal Antibody (Cat# AAA7006455)
Full Name
RASA1 Antibody
Gene Names
RASA1; GAP; PKWS; RASA; p120; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
Reactivity
Human
Applications
EIA, WB, IF
Purity
>95%,Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
Rac-GAP1, ELISA Kit (Cat# AAA2511371)
Full Name
Human Rac-GAP1 (Rac-GTPase Activating Protein 1) ELISA Kit
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western blot analysis of GIT2 expression in MCF7 (A) whole cell lysates.)
GIT2, Polyclonal Antibody (Cat# AAA8207516)
Full Name
Anti-GIT2 Antibody
Gene Names
GIT2; CAT2; CAT-2
Reactivity
Human, Chicken
Applications
WB
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$175/0.03 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
RACGAP1, cDNA Clone (Cat# AAA1266440)
Full Name
RACGAP1 cDNA Clone
Gene Names
RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: GIT1 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 85,20,86 KDaObserved band size: 85 KDa)
GIT1, Polyclonal Antibody (Cat# AAA7051985)
Full Name
GIT1 Antibody
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Testing Data
(Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Primary antibody diluted at 1: 1000)
Rho GAP4, Polyclonal Antibody (Cat# AAA9411037)
Full Name
Rho GAP4 antibody
Gene Names
ARHGAP4; C1; RGC1; p115; SrGAP4; RhoGAP4
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by antigen-affinity chromatography.
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Ras GTPase-activating protein SynGAP (SYNGAP1), Recombinant Protein (Cat# AAA1304855)
Full Name
Recombinant Human Ras GTPase-activating protein SynGAP (SYNGAP1) , partial
Gene Names
SYNGAP1; MRD5; RASA1; RASA5; SYNGAP
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $510/0.1 mg (E-Coli) | $310/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using Phospho-G3BP1-S232 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
G3BP1-S232, Antibody (Cat# AAA128331)
Full Name
Phospho-G3BP1-S232 Polyclonal Antibody
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using IQGAP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IQGAP3, Polyclonal Antibody (Cat# AAA9128941)
Full Name
IQGAP3 Polyclonal Antibody
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Lane1; Lane2: Mouse Brain Tissue; Lane3: Rat Brain Tissue.)
Ras GTPase Activating Protein 1, Polyclonal Antibody (Cat# AAA2026028)
Full Name
Polyclonal Antibody to Ras GTPase Activating Protein 1 (RASA1)
Gene Names
RASA1; GAP; PKWS; RASA; p120; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse thymus, using ARL6IP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
ARL6IP5, Polyclonal Antibody (Cat# AAA9134834)
Full Name
ARL6IP5 Polyclonal Antibody
Gene Names
ARL6IP5; JWA; jmx; hp22; PRAF3; Yip6b; DERP11; HSPC127; addicsin; GTRAP3-18
Reactivity
Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Phospho-GTPase Activating Protein (Ser387) Antibody expression in COS7 cells lysates.The lane on the right is treated with the antigen-specific peptide.)
GTPase Activating Protein, Polyclonal Antibody (Cat# AAA9601251)
Full Name
Phospho-GTPase Activating Protein (Ser387) Antibody
Gene Names
RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HL-60 cells, using G3BP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
G3BP2, Polyclonal Antibody (Cat# AAA9126015)
Full Name
G3BP2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat, Hamster
Applications
WB, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Immunofluorescence (IF)
(MBS9604975 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
AS250, Polyclonal Antibody (Cat# AAA9604975)
Full Name
AS250 Antibody
Gene Names
RALGAPA2; p220; AS250; C20orf74; dJ1049G11; bA287B20.1; dJ1049G11.4
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of G3BP-1 expression in 293 whole cell lysates)
G3BP-1, Polyclonal Antibody (Cat# AAA9601977)
Full Name
G3BP-1 Antibody
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebellum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse CHN2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Chimerin 2, Polyclonal Antibody (Cat# AAA2032766)
Full Name
Chimerin 2 (CHN2) Polyclonal Antibody
Gene Names
Chn2; Bch; ARHGAP3; 1700026N20Rik; 4930557O16Rik
Reactivity
Mouse
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $210/0.2 mL | $355/0.5 mL | $435/1 mL | $1,240/5x1 mL
Immunohistochemistry (IHC)
(MBS9608398 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
KHDRBS1, Polyclonal Antibody (Cat# AAA9608398)
Full Name
KHDRBS1 Antibody
Gene Names
KHDRBS1; p62; p68; Sam68
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9602054 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
ARHGAP1, Polyclonal Antibody (Cat# AAA9602054)
Full Name
ARHGAP1 Antibody
Gene Names
ARHGAP1; RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
G3BP, Monoclonal Antibody (Cat# AAA435094)
Full Name
G3BP Mouse mAb
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of IQGAP1 expression in Rat brain lysate )
IQGAP1, Polyclonal Antibody (Cat# AAA9605842)
Full Name
IQGAP1 Antibody
Gene Names
IQGAP1; SAR1; p195; HUMORFA01
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
