Rabbit anti-Human, Mouse GIT1 Polyclonal Antibody | anti-GIT1 antibody

GIT1 Antibody

Cat. Num. AAA7051985

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: GIT1; Polyclonal Antibody; GIT1 Antibody; ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; anti-GIT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 770

• Applicable Applications for anti-GIT1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human ARF GTPase-activating protein GIT1 protein (470-642AA)
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Immunogen Species: Homo sapiens (Human)
• Research Area: Signal Transduction
• Target Names: GIT1
• Preparation and Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse brain tissueAll lanes: GIT1 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 85,20,86 KDaObserved band size: 85 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7051985 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS7051985 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7051985 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-GIT1 antibody

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 146231998
• NCBI GeneID: 28964
• NCBI Accession #: NP_001078923.1
• NCBI GenBank Nucleotide #: NM_001085454.1
• UniProt Accession #: Q9Y2X7; Q86SS0; Q9BRJ4; B4DGU9; B4DSV3
• NCBI Official Full Name: ARF GTPase-activating protein GIT1 isoform 1
• NCBI Official Synonym Full Names: GIT ArfGAP 1
• NCBI Official Symbol: GIT1
• NCBI Protein Information: ARF GTPase-activating protein GIT1
• UniProt Protein Name: ARF GTPase-activating protein GIT1
• Protein Family: ARF GTPase-activating protein
• UniProt Gene Name: GIT1
• UniProt Synonym Gene Names: ARF GAP GIT1; CAT-1; CAT1

Uniprot Description

GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).

Protein type: GAPs; GAPs, ARF; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane

Molecular Function: protein binding

Biological Process: ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway