Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
3172 results for "Tuberculosis" - showing 1-50
Mycobacterium Tuberculosis major secretory protein Antigen 85B, Recombinant Protein (Cat# AAA143259)
Full Name
Recombinant Mycobacterium Tuberculosis major secretory protein Antigen 85B
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
$250/0.01 mg | $1,110/0.1 mg | $180/0.002 mg | $4,780/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
tuberculosis (TB) antibody (IgG), ELISA Kit (Cat# AAA702330)
Full Name
Human tuberculosis (TB) antibody, IgG ELISA Kit
Reactivity
Human
Pricing
$405/48-Strip-Wells | $570/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,470/5x96-Strip-Wells | $4,700/10x96-Strip-Wells
SDS-PAGE
Enoyl-[acyl-carrier-protein] reductase [NADH], Recombinant Protein (Cat# AAA1150692)
Full Name
Recombinant Mycobacterium tuberculosis Enoyl-[acyl-carrier-protein] reductase [NADH]
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $1,495/0.5 mg (Yeast) | $625/0.1 mg (E-Coli) | $685/0.1 mg (Yeast) | $365/0.02 mg (E-Coli) | $410/0.02 mg (Yeast) | $8,395/5x1 mg (E-Coli) | $6,555/5x0.5 mg (Yeast)
Testing Data
(Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN- gamma within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN- gamma and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.)
IFN GAMMA, Monoclonal Antibody (Cat# AAA215223)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin
Applications
EIA, FC/FACS
Pricing
$630/0.25 mg | $2,690/5x0.25 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
SDS-Page
Diacylglycerol acyltransferase/mycolyltransferase Ag85C (fbpC), Recombinant Protein (Cat# AAA1079141)
Full Name
Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85C (fbpC)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
ESAT6, Recombinant Protein (Cat# AAA204541)
Full Name
ESAT6, 1-95aa, Mycobacterium tuberculosis, His tag, Baculovirus
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
$230/0.01 mg | $405/0.05 mg | $985/0.25 mg | $4,180/5x0.25 mg
Mycobacterium tuberculosis, Polyclonal Antibody (Cat# AAA534825)
Full Name
Mycobacterium tuberculosis antibody
Applications
EIA, IHC, WB, IFA
Purity
> 95%
Pricing
$205/1 mg | $915/5x1 mg
SDS-PAGE
Diacylglycerol acyltransferase/mycolyltransferase Ag85B (fbpB), Recombinant Protein (Cat# AAA1017744)
Full Name
Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85B (fbpB)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $1,495/0.5 mg (Yeast) | $625/0.1 mg (E-Coli) | $685/0.1 mg (Yeast) | $365/0.02 mg (E-Coli) | $410/0.02 mg (Yeast) | $8,395/5x1 mg (E-Coli) | $6,555/5x0.5 mg (Yeast)
SDS-PAGE
Ba(Mycobacterium tuberculosis) Phosphate-binding protein PstS 1, Recombinant Protein (Cat# AAA1265435)
Full Name
Recombinant Ba(Mycobacterium tuberculosis) Phosphate-binding protein PstS 1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$365/0.02 mg | $625/0.1 mg | $1,915/1 mg
Western Blot (WB)
(Western BlotPositive WB detected in: mpt64 293TTransfected lysate, 293T non-Transfected lysateAll lanes: mpt64 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 51 kDaObserved band size: 51 kDa)
Mycobacterium tuberculosis Immunogenic protein MPT64, Polyclonal Antibody (Cat# AAA719734)
Full Name
Rabbit anti-Mycobacterium tuberculosis Immunogenic protein MPT64 polyclonal Antibody
Gene Names
mpt64; mpb64
Reactivity
MycoBacterium tuberculosis
Applications
EIA, WB
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-PAGE
Probable cutinase Rv1984c (Rv1984c), Recombinant Protein (Cat# AAA1060498)
Full Name
Recombinant Mycobacterium tuberculosis Probable cutinase Rv1984c (Rv1984c)
Gene Names
cfp21; clp1; culp1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
ESAT-6-like protein EsxH, Recombinant Protein (Cat# AAA1103807)
Full Name
Recombinant Mycobacterium tuberculosis ESAT-6-like protein EsxH
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-Page
Hypoxic response protein 1 (hrp1), Recombinant Protein (Cat# AAA1070643)
Full Name
Recombinant Mycobacterium tuberculosis Hypoxic response protein 1 (hrp1)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
Recombination protein RecR (recR), Recombinant Protein (Cat# AAA1092958)
Full Name
Recombinant Mycobacterium tuberculosis Recombination protein RecR (recR)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
Antitoxin MT2731 (MT2731), Recombinant Protein (Cat# AAA1085329)
Full Name
Recombinant Mycobacterium tuberculosis Antitoxin MT2731 (MT2731)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$685/0.1 mg (Yeast) | $410/0.02 mg (Yeast) | $2,930/5x0.1 mg (Yeast)
Western Blot (WB)
(Western blotAll lanes: Antigen 85-A antibody at 2ug/mlLane 1: Recombinant Mycobacterium tuberculosis Antigen 85-A protein 1ugLane 2: Recombinant Mycobacterium tuberculosis Antigen 85-A protein 10ugSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 36kDaObserved band size: 36kDa)
Mycobacterium tuberculosis Antigen 85-A, Polyclonal Antibody (Cat# AAA1490476)
Full Name
Rabbit anti-Mycobacterium tuberculosis Antigen 85-A polyclonal Antibody
Gene Names
fbpA; 85A; mpt44
Reactivity
MycoBacterium tuberculosis
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
Putative amidase AmiB2 (amiB2), Recombinant Protein (Cat# AAA1013003)
Full Name
Recombinant Putative amidase AmiB2 (amiB2)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
Steroid C26-monooxygenase, Recombinant Protein (Cat# AAA1080108)
Full Name
Recombinant Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Steroid C26-monooxygenase
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $1,495/0.5 mg (Yeast) | $625/0.1 mg (E-Coli) | $685/0.1 mg (Yeast) | $365/0.02 mg (E-Coli) | $410/0.02 mg (Yeast) | $8,395/5x1 mg (E-Coli) | $6,555/5x0.5 mg (Yeast)
Testing Data
(Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN- gamma within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN- gamma and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.)
IFN GAMMA, Monoclonal Antibody (Cat# AAA215222)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA
Reactivity
Dog, Dolphin, Ferret, Fin Whale, Goat, Horse, Human, Mink, Rabbit, Pig, Sheep.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Based on sequence similarity, is expected to react with: Mustelid
N.B. Antibody reactivity and working conditions may vary between species.
Applications
EIA, FC
Pricing
$680/0.5 mg | $2,900/5x0.5 mg
tuberculosis antibody IgM (TB-Ab IgM), ELISA Kit (Cat# AAA917256)
Full Name
Human tuberculosis antibody IgM, TB-Ab IgM ELISA Kit
Reactivity
Human
Pricing
$405/48-Strip-Wells | $570/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,470/5x96-Strip-Wells | $4,700/10x96-Strip-Wells
SDS-PAGE
Adenylate kinase (adk), Recombinant Protein (Cat# AAA1182254)
Full Name
Recombinant Mycobacterium tuberculosis Adenylate kinase (adk)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-Page
Low molecular weight antigen MTB12 (mtb12), Recombinant Protein (Cat# AAA1002640)
Full Name
Recombinant Mycobacterium tuberculosis Low molecular weight antigen MTB12 (mtb12)
Gene Names
cfp2; mtb12
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$2,235/1 mg (Yeast) | $685/0.1 mg (Yeast) | $410/0.02 mg (Yeast) | $10,040/5x1 mg (Yeast)
SDS-Page
Mycothione reductase (mtr), Recombinant Protein (Cat# AAA1239474)
Full Name
Recombinant Mycothione reductase (mtr)
Gene Names
mtr; gorA
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
Glutamate decarboxylase 1 (GAD1) autoantibody, ELISA Kit (Cat# AAA706472)
Full Name
Human Glutamate decarboxylase 1, GAD1 autoantibody ELISA Kit
Gene Names
GAD; GAD1; glutamate decarboxylase; GLUTAMATE DECARBOXYLASE; GLUTAMATE DECARBOXYLASE 1; MKP11.30; MKP11_30
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells
SDS-PAGE
Signal peptidase I, Recombinant Protein (Cat# AAA1302881)
Full Name
Recombinant Mycobacterium tuberculosis Signal peptidase I
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $1,495/0.5 mg (Yeast) | $625/0.1 mg (E-Coli) | $685/0.1 mg (Yeast) | $365/0.02 mg (E-Coli) | $410/0.02 mg (Yeast) | $8,395/5x1 mg (E-Coli) | $6,555/5x0.5 mg (Yeast)
SDS-Page
MPT51/MPB51 antigen, Recombinant Protein (Cat# AAA1182334)
Full Name
Recombinant Mycobacterium tuberculosis MPT51/MPB51 antigen
Gene Names
fbpD; fbpC1; mpb51; mpt51
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
Diacylglycerol acyltransferase/mycolyltransferase Ag85A (fbpA), Recombinant Protein (Cat# AAA1265459)
Full Name
Recombinant Mycobacterium tuberculosis Diacylglycerol acyltransferase/mycolyltransferase Ag85A (fbpA), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$365/0.02 mg | $975/0.2 mg | $4,185/5x0.2 mg
SDS-PAGE
Toxin RelG (relG), Recombinant Protein (Cat# AAA1050448)
Full Name
Recombinant Toxin RelG (relG)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
Mycobacterium tuberculosis ESAT-6-like protein EsxB, Polyclonal Antibody (Cat# AAA1491361)
Full Name
Rabbit anti-Mycobacterium tuberculosis ESAT-6-like protein EsxB polyclonal Antibody
Gene Names
esxB; cfp10; lhp
Reactivity
MycoBacterium tuberculosis
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-PAGE
Thymidylate kinase, Recombinant Protein (Cat# AAA1005731)
Full Name
Recombinant Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) Thymidylate kinase
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
SDS-PAGE
Immunogenic protein MPT63, Recombinant Protein (Cat# AAA1265196)
Full Name
Recombinant Mycobacterium tuberculosis Immunogenic protein MPT63 protein
Gene Names
mpt63; mpb63
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$365/0.02 mg | $625/0.1 mg | $1,915/1 mg | $8,395/5x1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Testing Data
(Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN- gamma within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN- gamma and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.)
IFN GAMMA, Monoclonal Antibody (Cat# AAA215224)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:FITC
Applications
FC/FACS
Pricing
$630/0.1 mg | $2,690/5x0.1 mg
SDS-PAGE
Malate synthase G (glcB), partial, Recombinant Protein (Cat# AAA1037879)
Full Name
Recombinant Mycobacterium tuberculosis Malate synthase G (glcB), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$2,040/1 mg (E-Coli) | $640/0.1 mg (E-Coli) | $440/0.02 mg (E-Coli) | $8,950/5x1 mg (E-Coli)
Escherichia Coli Elongation Factor TU, Monoclonal Antibody (Cat# AAA631756)
Full Name
Escherichia Coli Elongation Factor TU (EF-TU)
Applications
EL/EIA, WB, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Pricing
$715/0.1 mg | $3,205/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
luteinizing hormone (LH), ELISA Kit (Cat# AAA706567)
Full Name
Monkey luteinizing hormone, LH ELISA Kit
Reactivity
Monkey
Pricing
$455/48-Strip-Wells | $640/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,650/5x96-Strip-Wells | $5,045/10x96-Strip-Wells
Testing Data
(Published customer image: Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN- gamma within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN- gamma and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.)
IFN GAMMA, Monoclonal Antibody (Cat# AAA215225)
Full Name
MOUSE ANTI BOVINE INTERFERON GAMMA:RPE
Applications
FC/FACS
Pricing
$710/100 Tests | $3,035/5x100 Tests
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Western Blot (WB)
(Anti-TLR2 Antibody (C226) at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ?g per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
TLR2, Polyclonal Antibody (Cat# AAA9211881)
Full Name
TLR2 Antibody (N-term)
Gene Names
TLR2; TIL4; CD282
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, FC
Purity
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Methionine aminopeptidase 1 (map-1), Recombinant Protein (Cat# AAA1474756)
Full Name
Recombinant Mycobacterium tuberculosis Methionine aminopeptidase 1 (map-1)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,915/1 mg (E-Coli) | $625/0.1 mg (E-Coli) | $365/0.02 mg (E-Coli) | $8,395/5x1 mg (E-Coli)
Immunohistochemistry (IHC)
(MBS9603409 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
VPS33B, Polyclonal Antibody (Cat# AAA9603409)
Full Name
VPS33B Antibody
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of TSC-22R using HepG2 whole cell lysates)
TSC-22R, Polyclonal Antibody (Cat# AAA9602936)
Full Name
TSC-22R Antibody
Gene Names
TSC22D3; DIP; GILZ; DSIPI; TSC-22R
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Mycobacterium tuberculosis CFP10 (10kDa culture filtrate protein), Monoclonal Antibody (Cat# AAA312553)
Full Name
MAb to M. tuberculosis CFP10
Applications
EIA
Purity
>95% pure. Protein G chromatography
Pricing
$795/1 mg | $3,430/5x1 mg
Myobacterium Tuberculosis DnaK, Recombinant Protein (Cat# AAA142708)
Full Name
Recombinant Myobacterium Tuberculosis DnaK (HSP70)
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
$250/0.01 mg | $1,400/0.1 mg | $180/0.002 mg | $6,085/5x0.1 mg
Mycobacterium tuberculosis, Hsp65, Monoclonal Antibody (Cat# AAA310272)
Full Name
MAb to M. tuberculosis
Applications
EIA/ELISA, Western Blot
Purity
>90% pure. Protein A chromatography
Pricing
$1,025/1 mg | $4,460/5x1 mg
Mycobacterium tuberculosis (all antigens), Polyclonal Antibody (Cat# AAA315163)
Full Name
Rabbit A' M. tuberculosis
Applications
Immunohistochemistry - Paraffin; IFA
Purity
>95% pure. Protein A chromatography
Pricing
$530/1 mL | $2,235/5x1 mL
Mycobacterium tuberculosis, 38kDa, Recombinant, Recombinant Protein (Cat# AAA319392)
Full Name
M. tuberculosis, 38kDa, Recombinant
Applications
ELISA
Purity
95% pure (SDS-PAGE)
Pricing
$755/0.1 mg | $3,245/5x0.1 mg
