Typical Testing Data/Standard Curve (for reference only)
Mouse Beta-1, 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase 3 (GCNT3) ELISA Kit | Gcnt3 elisa kit
Mouse Beta-1, 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase 3 (GCNT3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GCNT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCNT3 antibody and an GCNT3-HRP conjugate. The assay sample and buffer are incubated together with GCNT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCNT3 concentration since GCNT3 from samples and GCNT3-HRP conjugate compete for the anti-GCNT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GCNT3 from the sample, fewer sites are left to bind GCNT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCNT3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.
Protein type: EC 2.4.1.102; Membrane protein, integral; Transferase; EC 2.4.1.150; Glycan Metabolism - O-glycan biosynthesis
Cellular Component: Golgi apparatus; membrane; integral to membrane
Molecular Function: transferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity
Biological Process: protein amino acid O-linked glycosylation; tissue morphogenesis; immunoglobulin production in mucosal tissue; intestinal absorption
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Product Notes
The Mouse Gcnt3 gcnt3 (Catalog #AAA7210159) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7210159 ELISA Kit recognizes Mouse Gcnt3. It is sometimes possible for the material contained within the vial of "Beta-1, 3-galactosyl-O-glycosyl-glycoprotein beta-1, 6-N-acetylglucosaminyltransferase 3 (GCNT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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