Rabbit TLR2 Polyclonal Antibody | anti-TLR2 antibody

TLR2 Antibody (N-term)

Cat. Num. AAA9211881

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry
• Purity: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Synonyms: TLR2; Polyclonal Antibody; TLR2 Antibody (N-term); Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; CD282; TIL4; anti-TLR2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TLR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-242 amino acids from the N-terminal region of human TLR2.
• Purity/Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 212-242
• Sequence Length: 784

• Applicable Applications for anti-TLR2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC)
• Application Notes: WB~~1:1000; IHC-P~~1:10~50; FC~~1:10~50
• Function: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852, PubMed:17889651). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins (PubMed:10426995, PubMed:10426996). Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6 (PubMed:11441107). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4 (PubMed:16622205). MAPK activation in response to bacterial peptidoglycan also occurs via this receptor (PubMed:16622205). Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36); the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712). M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression (PubMed:15809303). Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse) (By similarity). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides (PubMed:16880211). Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM (By similarity).
• Cellular Location: Membrane {ECO:0000250|UniProtKB:Q9QUN7}; Single- pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q9QUN7}; Single-pass type I membrane protein. Membrane raft. Note=Does not reside in lipid rafts before stimulation but accumulates increasingly in the raft upon the presence of the microbial ligand. In response to diacylated lipoproteins, TLR2:TLR6 heterodimers are recruited in lipid rafts, this recruitment determines the intracellular targeting to the Golgi apparatus. Triacylated lipoproteins induce the same mechanism for TLR2:TLR1 heterodimers.
• Tissue Location: Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-TLR2 Antibody (C226) at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ?g per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with TLR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC)

(Flow cytometric analysis of CEM cells using TLR2 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TLR2 antibody

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB.

Product Categories/Family for anti-TLR2 antibody

Cardiovascular; Immunology; Microbiology; Signal Transduction

References

Meng, G., et al., J. Biol. Chem. 278(41):39822-39829 (2003). Sandor, F., et al., J. Cell Biol. 162(6):1099-1110 (2003). Wang, X., et al., FASEB J. 17(12):1727-1729 (2003). Huang, L.Y., et al., J. Immunol. 171(3):1441-1446 (2003). Takeuchi, J., et al., Biochem. Biophys. Res. Commun. 306(3):674-679 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 19718734
• NCBI GeneID: 7097
• NCBI Accession #: NP_003255.2
• NCBI GenBank Nucleotide #: NM_003264.3
• UniProt Accession #: O60603; O15454; Q8NI00; B3Y612; D1CS45; D1CS48; D1CS49
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral; Cell surface; Apoptosis

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell projection; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; protein heterodimerization activity; triacylated lipoprotein binding; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-12 production; apoptosis; response to toxin; positive regulation of leukocyte migration; microglial cell activation; leukotriene metabolic process; positive regulation of NF-kappaB import into nucleus; detection of triacylated bacterial lipoprotein; response to molecule of fungal origin; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of interleukin-17 production; myelin formation in the central nervous system; toll-like receptor signaling pathway; response to hypoxia; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; I-kappaB phosphorylation; chloramphenicol transport; positive regulation of inflammatory response; positive regulation of cytokine secretion; positive regulation of nitric-oxide synthase biosynthetic process

Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To