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15 results for "T cell Mediated Autoimmunity" - showing 1-15


Flow Cytometry (FC/FACS (FCM staining of human PBMC's using CD46 Monoclonal Antibody (122.2).)

CD46, Monoclonal Antibody (Cat# AAA438295)

Full Name
CD46 (Membrane Cofactor Protein) Mouse Monoclonal Antibody
Gene Names
CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
Reactivity
Human
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data (Cluster Of Differentiation 276 (CD276) also known as B7-H3 in human, is a member of the B7/CD28 superfamily of costimulatory molecules serving as an accessory modulator of T-cell response. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production. Besides, Sialic acid-binding Ig-like lectin 12 (SIGLEC12) has been identified as an interactor of CD276, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD276 and recombinant human SIGLEC12. Briefly, CD276 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC12-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD276 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CD276 and SIGLEC12 was shown in Figure 1, and this effect was in a dose dependent manner.)

Cluster Of Differentiation 276 (CD276), Active Protein (Cat# AAA2097281)

Full Name
Active Cluster Of Differentiation 276 (CD276)
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg

Valpha TCR, Recombinant Antibody (Cat# AAA488206)

Full Name
Anti-Valpha TCR [34]
Reactivity
Human
Applications
EIA, FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Anti-CEACAM Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CEACAM, Polyclonal Antibody (Cat# AAA9204586)

Full Name
CEACAM Antibody (N-term)
Gene Names
CEACAM1; BGP; BGP1; BGPI
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL

Valpha TCR, Recombinant Antibody (Cat# AAA488207)

Full Name
Anti-Valpha TCR [34]
Reactivity
Human
Applications
EIA, FC/FACS, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg

TNF, Polyclonal Antibody (Cat# AAA1488703)

Full Name
Rabbit Anti Human TNF PolyClonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$320/0.1 mL | $1,440/5x0.1 mL

CD5L / CD5 Antigen-Like, Recombinant Protein (Cat# AAA2903333)

Full Name
Human CD5L / CD5 Antigen-Like Recombinant Protein
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE

ARID5A, Polyclonal Antibody (Cat# AAA9630008)

Full Name
ARID5A Antibody
Gene Names
ARID5A; MRF1; MRF-1; RP11-363D14
Reactivity
Human
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (Immunofluorescence staining of U937 cells using anti-CD20 antibody (MBS488637) 18B12. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 18B12 (MBS488637) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488637, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)

CD20, Monoclonal Antibody (Cat# AAA488636)

Full Name
Anti-CD20 [18B12]
Gene Names
Ms4a1; Cd20; Ly-44; Ms4a2; AA960661
Reactivity
Mouse
Applications
FC/FACS, IF, WB, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-CD80 antibody RM80 (B467) (MBS488602) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RM80 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

CD80, Monoclonal Antibody (Cat# AAA488602)

Full Name
Anti-CD80 [RM80]
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

CD86, Monoclonal Antibody (Cat# AAA488604)

Full Name
Anti-CD86 [PO.3]
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 (MBS488604) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488604, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

CD86, Monoclonal Antibody (Cat# AAA488605)

Full Name
Anti-CD86 [PO.3]
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of fixed RAW264.7 cells with anti-CD80 antibody RM80 (B467) (MBS488602) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of RM80 (MBS488602) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488602, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)

CD80, Monoclonal Antibody (Cat# AAA488603)

Full Name
Anti-CD80 [RM80]
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Applications
FC/FACS, WB, IHC, BL, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF) (Immunofluorescence staining of U937 cells using anti-CD20 antibody (MBS488637) 18B12. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of 18B12 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)

CD20, Monoclonal Antibody (Cat# AAA488637)

Full Name
Anti-CD20 [18B12]
Gene Names
Ms4a1; Cd20; Ly-44; Ms4a2; AA960661
Reactivity
Mouse
Applications
FC/FACS, IF, WB, FA
Purity
Purified antibody. Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg

RC3H1, Polyclonal Antibody (Cat# AAA9630994)

Full Name
RC3H1 Antibody
Gene Names
RC3H1; RNF198; ROQUIN
Reactivity
Human, Mouse
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
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