Rabbit anti-Human ARID5A Polyclonal Antibody | anti-ARID5A antibody
ARID5A Antibody
Gene Names
ARID5A; MRF1; MRF-1; RP11-363D14
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
ARID5A; Polyclonal Antibody; ARID5A Antibody; ARID domain containing protein 5A; ARID-containing protein 5A; AT rich interactive domain 5A; AT rich interactive domain 5A (MRF1 like); AT rich interactive domain containing protein 5A; Modulator recognition factor 1; MRF 1; MRF1; RFVG5814; RP11 363D14; anti-ARID5A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
ARID5A Antibody detects endogenous levels of ARID5A.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ARID5A antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human ARID5A.
Conjugation
Unconjugated
Post Translational Modifications
Phosphorylated by MAPK14 on serine residues involving a TLR4 signaling pathway upon lipopolysaccharide (LPS) stimulation leading to its ubiquitination and proteasomal degradation.Ubiquitinated leading to proteasomal degradation; involving WWP1 linked to MAPK14-mediated phosphorylation upon LPS stimulation.
Subunit Structure
Interacts with SOX9 (By similarity). Interacts with ESR1. Interacts with RORC (By similarity).
Function
Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer-dependent gene expressison. May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (By similarity). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors. As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3'UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells. In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling. Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (By similarity). Stabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR; thereby competing with the mRNA-destabilizing functions of RC3H1 and endoribonuclease ZC3H12A (By similarity).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,074 Da
NCBI Official Full Name
AT-rich interactive domain-containing protein 5A
NCBI Official Synonym Full Names
AT rich interactive domain 5A (MRF1-like)
NCBI Official Symbol
ARID5A
NCBI Official Synonym Symbols
MRF1; MRF-1; RP11-363D14
NCBI Protein Information
AT-rich interactive domain-containing protein 5A; ARID domain-containing protein 5A; RFVG5814; modulator recognition factor 1; modulator recognition factor I
UniProt Protein Name
AT-rich interactive domain-containing protein 5A
Protein Family
UniProt Gene Name
ARID5A
UniProt Synonym Gene Names
MRF1; ARID domain-containing protein 5A; MRF-1
UniProt Entry Name
ARI5A_HUMAN
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Product Notes
The ARID5A arid5a (Catalog #AAA9630008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARID5A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARID5A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the ARID5A arid5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARID5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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