CD5L / CD5 Antigen-Like Recombinant Protein | CD5L recombinant protein

Human CD5L / CD5 Antigen-Like Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: CD5L / CD5 Antigen-Like; Human CD5L / CD5 Antigen-Like Recombinant Protein; CD5 antigen-like; Apoptosis inhibitor expressed by macrophages; hAIM; CT-2; IgM-associated peptide; SP-alpha; API6; UNQ203/PRO229; CD5L recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 347

• Applicable Applications for CD5L recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with FASN; the interaction is direct (By similarity). Interacts with IgM; protecting CD5L from renal excretion and leading to increased CD5L levels in circulating blood (PubMed:8034987, PubMed:24804991).
• Entry Function: Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflammed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (By similarity). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:24295828). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (PubMed:16030018, PubMed:24223991, PubMed:24583716, PubMed:25713983).
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 5174411
• NCBI Accession #: NP_005885.1
• NCBI GenBank Nucleotide #: NP_005885.1
• UniProt Accession #: O43866
• NCBI Official Full Name: CD5 antigen-like isoform 1
• UniProt Protein Name: CD5 antigen-like
• Protein Family: CD5 antigen
• UniProt Gene Name: CD5L
• UniProt Synonym Gene Names: API6; hAIM
• UniProt Entry Name: CD5L_HUMAN

Uniprot Description

CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.

Protein type: Receptor, misc.; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: extracellular space

Biological Process: cellular defense response