Rabbit anti-Human, Mouse RC3H1 Polyclonal Antibody | anti-RC3H1 antibody

RC3H1 Antibody

Cat. Num. AAA9630994

• Gene Names: RC3H1; RNF198; ROQUIN
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RC3H1; Polyclonal Antibody; RC3H1 Antibody; KIAA2025; Rc3h1; RC3H1_HUMAN; RGD1563512; RING finger and C3H zinc finger protein 1; Ring Finger and CCCH type Zinc Finger Domain 1; RING finger protein 198; RNF198; Roquin; RP5 1198E17.5; Sanroque protein; anti-RC3H1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RC3H1 Antibody detects endogenous levels of RC3H1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RC3H1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human RC3H1.
• Conjugation: Unconjugated
• Similarity: The RING-type zinc finger is required for proper localization to stress granules, but not to P-bodies. The ROQ region is required for CDE RNA-binding (PubMed:25504471, PubMed:25026078). Has 2 separate RNA-binding sites, one for CDE RNA and the other for dsRNA, both sites are important for mRNA decay (PubMed:25026078). ADE RNA-binding involves an extended binding surface on the ROQ region with a number of additional residues compared with the CDE RNA (By similarity). It may also be involved in localization to stress granules (By similarity). HEPN (higher eukaryotes and prokaryotes nucleotide-binding) are observed in both N- and C-terminal sides of ROQ domain with 3D structure even if they are poredcted on the basis of sequence.
• Post Translational Modifications: Proteolytically cleaved after Arg-510 and Arg-579 by MALT1 in activated CD4(+) T cells; cleavage at Arg-510 and Arg-579 is critical for promoting RC3H1 degradation in response to T-cell receptor (TCR) stimulation, and hence is necessary for prolonging the stability of a set of mRNAs controlling Th17 cell differentiation.
• Subunit Structure: Able to homodimerize. Interacts with DDX6 and EDC4. Interacts with CCR4-NOT deadenylase complex (By similarity). Interacts with RC3H1; the interaction is RNA independent.
• Tissue Specificity: Widely expressed. Expressed at higher level in cerebellum, spleen, ovary and liver.
• Function: Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF, TNFRSF4 and in many more mRNAs. Cleaves translationally inactive mRNAs harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-independent manner (By similarity). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity (By similarity). In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression (By similarity). Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation (By similarity). Recognizes and binds mRNAs containing a hexaloop stem-loop motif, called alternative decay element (ADE) (By similarity). Together with ZC3H12A, destabilizes TNFRSF4/OX40 mRNA by binding to the conserved stem loop structure in its 3'UTR (By similarity). Able to interact with double-stranded RNA (dsRNA). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression. Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2A, UBE2B, UBE2D2, UBE2F, UBE2G1, UBE2G2 and UBE2L3 and produces polyubiquitin chains. Shows the strongest activity when paired with UBE2N:UBE2V1 or UBE2N:UBE2V2 E2 complexes and generate both short and long polyubiquitin chains.
• Note: During stress, such as that induced by arsenite treatment, localizes to cytosolic stress granules (By similarity). Localization to stress granules, but not to P-bodies, depends upon the RING-type zinc finger (By similarity). ICOS repression may correlate with the localization to P-bodies, not to stress granules (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-RC3H1 antibody

Cytoplasm; P-body; Cytoplasmic granule; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 73695473
• NCBI GeneID: 149041
• NCBI Accession #: NP_742068
• NCBI GenBank Nucleotide #: NM_172071.3
• UniProt Accession #: Q5TC82; Q5W180; Q5W181; Q8IVE6; Q8N9V1; B3KVK1
• Molecular Weight: 125 kDa
• NCBI Official Full Name: roquin-1 isoform b
• NCBI Official Synonym Full Names: ring finger and CCCH-type domains 1
• NCBI Official Symbol: RC3H1
• NCBI Official Synonym Symbols: RNF198; ROQUIN
• NCBI Protein Information: roquin-1; RING finger protein 198; RING finger and C3H zinc finger protein 1; probable E3 ubiquitin-protein ligase Roquin; RING finger and CCCH-type zinc finger domain-containing protein 1
• UniProt Protein Name: Roquin-1
• Protein Family: Roquin
• UniProt Gene Name: RC3H1
• UniProt Synonym Gene Names: KIAA2025; RNF198; Roquin
• UniProt Entry Name: RC3H1_HUMAN

NCBI Description

This gene encodes a protein containing RING-type and C3H1-type zinc finger motifs. The encoded protein recognizes and binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs, leading to mRNA deadenylation and degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

RC3H1: Represses ICOS function, an essential costimulatory receptor for follicular T-cells. May act as an ubiquitin ligase that regulates ubiquitin-dependent control of AU-rich mRNAs by AUF1/HNRPD and HUR/ELAVL1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q25.1

Cellular Component: stress granule

Molecular Function: mRNA 3'-UTR binding; zinc ion binding

Biological Process: spleen development; negative regulation of germinal center formation; negative regulation of activated T cell proliferation; protein ubiquitination; negative regulation of B cell proliferation; regulation of mRNA stability; lymph node development; regulation of germinal center formation; T cell proliferation; negative regulation of T-helper cell differentiation; B cell homeostasis; regulation of T cell receptor signaling pathway; cytoplasmic mRNA processing body assembly; T cell homeostasis