Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
5605 results for " DNA Replication" - showing 1-50
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Western Blot (WB)
(Western Blot analysis of 293T, A549, Raji, K562 and NIH/3T3 cell using MCM6 Polyclonal Antibody at dilution of 1:433.3)
MCM6, Polyclonal Antibody (Cat# AAA2525714)
Full Name
MCM6 Polyclonal Antibody
Gene Names
mcm6; mis5; mmcm6
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
RPA-70, ELISA Kit (Cat# AAA2509013)
Full Name
Human RPA-70 (replication protein A) ELISA Kit
Gene Names
RpA-70; CG9633; D-RPA70; D-SSB; DmelCG9633; DmRPA; dmrpa1; dRP-A; DRP-A; i164; RP-A; RPA; RPA 70; RPA 70 kDa; Rpa-70; RpA1; RPA1; RpA70; RPA70; Ssb-70
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western Blot: Sample: Lane1: Human K562 Cells; Lane2: Human Hela Cells; Lane3: Human Jurkat Cells.)
Minichromosome Maintenance Deficient 2, Polyclonal Antibody (Cat# AAA2026581)
Full Name
Polyclonal Antibody to Minichromosome Maintenance Deficient 2 (MCM2)
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
Western Blot (WB)
(Western blot analysis of PCNA Antibody (C-term) in Jurkat, Hela, 293 cell line lysates (35ug/lane). PCNA (arrow) was detected using the purified Pab.)
PCNA, Polyclonal Antibody (Cat# AAA9206515)
Full Name
PCNA Antibody (C-term)
Gene Names
PCNA; ATLD2
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blotAll lanes: WD repeat-containing protein 54 at 2ug/ml+mouse stomach tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 36kDaObserved band size: 36kDa)
WD repeat-containing protein 54, Polyclonal Antibody (Cat# AAA1489930)
Full Name
Rabbit anti-human WD repeat-containing protein 54 polyclonal Antibody
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Geminin, GMNN, ELISA Kit (Cat# AAA2602751)
Full Name
Human Geminin, GMNN ELISA Kit
Gene Names
GMNN; Gem
Reactivity
Human
Pricing
$275/48-Strip-Wells | $395/96-Strip-Wells | $1,660/5x96-Strip-Wells | $3,030/10x96-Strip-Wells
GAPDH, Polyclonal Antibody (Cat# AAA9205368)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(All lanes: DNA replication licensing factor MCM6 antibody at 3ug/ml+Jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 93kDaObserved band size: 93kDa)
DNA replication licensing factor MCM6, Polyclonal Antibody (Cat# AAA1499335)
Full Name
Rabbit anti-human DNA replication licensing factor MCM6 polyclonal Antibody(MCM6)
Gene Names
MCM6; Mis5; P105MCM; MCG40308
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
SDS-PAGE (SDS)
(DS-PAGE Analysis Purified EBV Mouse Monoclonal Antibody. Confirmation of Purity and Integrity of Antibody.)
EBV Early Antigens, Monoclonal Antibody (Cat# AAA438342)
Full Name
EBV Early Antigens
Reactivity
EBV
Applications
IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500)
MCM6, Polyclonal Antibody (Cat# AAA2526839)
Full Name
MCM6 Polyclonal Antibody
Gene Names
mcm6; mis5; mmcm6
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: MCM2 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 102 KDaObserved band size: 102 KDa)
DNA replication licensing factor MCM2, Polyclonal Antibody (Cat# AAA7003843)
Full Name
Rabbit anti-human DNA replication licensing factor MCM2 polyclonal Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(All lanes: MCM3 antibody at 4 ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: K562 whole cell lysateLane 4:HepG2 whole cell lysateLane 5:Jurkats whole cell lysateLane 6:U917 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 91,96 kDaObserved band size: 91 kDa)
DNA replication licensing factor MCM3, Polyclonal Antibody (Cat# AAA1498914)
Full Name
Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3)
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human
Applications
EIA, WB, IHC, ChIP
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human lung cancer tissue using MCM2 Polyclonal Antibody at dilution 1:50)
MCM2, Polyclonal Antibody (Cat# AAA2524343)
Full Name
MCM2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Pancreas stained with ATRX Mouse Monoclonal Antibody (39f).)
ATRX/RAD54, Monoclonal Antibody (Cat# AAA4380174)
Full Name
ATRX/RAD54 (Alpha Thalassemia Mental Retardation)
Gene Names
ATRX; JMS; XH2; XNP; MRX52; RAD54; RAD54L; ZNF-HX
Reactivity
Human. Others not known.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(All lanes: DNA replication licensing factor MCM5 antibody at 3ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateLane 2:mouse thymus tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 82kDaObserved band size: 82kDa)
DNA replication licensing factor MCM5, Polyclonal Antibody (Cat# AAA1493689)
Full Name
Rabbit anti-human DNA replication licensing factor MCM5 polyclonal Antibody(MCM5)
Gene Names
MCM5; CDC46; P1-CDC46
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
Antigen Affinity Purified
Pricing
$180/0.05 mL | $260/0.1 mL | $1,160/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Double Stranded DNA Monoclonal Antibody (121-3))
Double Stranded DNA (dsDNA), Monoclonal Antibody (Cat# AAA438724)
Full Name
Double Stranded DNA (dsDNA) (Nuclear Marker) Mouse Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot analysis of various cells using BM28 Polyclonal Antibody at dilution of 1:2000.)
BM28, Polyclonal Antibody (Cat# AAA2538862)
Full Name
BM28 Polyclonal Antibody
Gene Names
Mcm2; BM28; CDCL1; Mcmd2; AA959861; AW476101; mKIAA0030
Reactivity
Human, Mouse
Applications
WB, IHC, IF, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
REPIN1, ELISA Kit (Cat# AAA2510142)
Full Name
Human REPIN1 (Replication Initiator 1) ELISA Kit
Gene Names
REPIN1; AP4; RIP60; ZNF464; Zfp464
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Cervix stained with HSVI Mouse Monoclonal Antibody (HSVI/2095).)
HSV1 (Herpes Simplex Virus Type I), Monoclonal Antibody (Cat# AAA4381514)
Full Name
HSV1 (Herpes Simplex Virus Type I)
Reactivity
HSV1 (Herpes Simplex Virus 1)
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot detection of RPA70/RPA1 in Raji, HCT116, Jurkat, MCF7 and Hela cell lysates using RPA70/RPA1 mouse mAb (dilution 1:1000).Predicted band size:68kDa.Observed band size:70kDa.)
RPA70/RPA1, Monoclonal Antibody (Cat# AAA476285)
Full Name
Anti-RPA70/RPA1 Mouse mAb
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human, Monkey
Applications
WB, ICH, ICC, FC
Purity
Affinity purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
Western Blot (WB)
(Western blot detection of PCNA in Hela, 3T3, COS7, C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)
PCNA, Monoclonal Antibody (Cat# AAA475080)
Full Name
Anti-PCNA Mouse mAb
Gene Names
PCNA; ATLD2
Reactivity
Human, Mouse, Monkey, Rat, Hamster
Applications
WB, IP, ICC, IHC
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of 293T, hela, K562, Raji and 231 cell using MCM2 Polyclonal Antibody at dilution of 1:400)
MCM2, Polyclonal Antibody (Cat# AAA2516475)
Full Name
MCM2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Double Stranded DNA Monoclonal Antibody (SPM603))
Double Stranded DNA (dsDNA), Monoclonal Antibody (Cat# AAA439089)
Full Name
Double Stranded DNA (dsDNA) (Nuclear Marker) Mouse Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Detection limit for recombinant GST tagged SETX is 0.3ng/ml using MBS641490 as a capture antibody.)
Senataxin, Monoclonal Antibody (Cat# AAA641490)
Full Name
Senataxin (Probable Helicase Senataxin, SETX, Amyotrophic Lateral Sclerosis 4 Protein, ALS4, AOA2, bA479K20.2, KIAA0625, SCAR1, SEN1 Homolog)
Gene Names
SETX; ALS4; AOA2; SCAR1; bA479K20.2
Reactivity
Human
Applications
IF, EIA, WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$620/0.1 mg | $2,775/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7003456 at dilution of 1:100)
GMNN, Polyclonal Antibody (Cat# AAA7003456)
Full Name
GMNN Antibody
Gene Names
GMNN; Gem; MGORS6
Reactivity
Human
Applications
EIA, IHC
Purity
Affinity Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using MBS7049024 at dilution of 1:100)
RPA1, Polyclonal Antibody (Cat# AAA7049024)
Full Name
RPA1 Antibody
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human
Applications
EIA, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Hela whole cell lysates, using UHRF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
UHRF1, Polyclonal Antibody (Cat# AAA9607582)
Full Name
UHRF1 Antibody
Gene Names
UHRF1; Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, IP, EIA, ChIP
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
FMR1, Polyclonal Antibody (Cat# AAA9207834)
Full Name
FMR1 Antibody (N-term)
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, mouse
Applications
IHC-P, WB, FC,E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Gallbladder stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).)
Topoisomerase (DNA) I, Mitochondrial (TOP1MT), Monoclonal Antibody (Cat# AAA438648)
Full Name
Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Mouse Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Sample(30 ug whole cell lysate)A: H1299B: HeLa S37.5% SDS PAGEPrimary antibody diluted at 1: 1000)
RPA 70 kDa subunit, Polyclonal Antibody (Cat# AAA9401646)
Full Name
RPA 70 kDa subunit antibody
Gene Names
RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by antigen-affinity chromatography.
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (SPM379).)
MCM7/CDC47, Monoclonal Antibody (Cat# AAA4380107)
Full Name
MCM7/CDC47 (Proliferation Marker)
Gene Names
MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
Reactivity
Human. Others not known
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Recommended PCNA Antibody Titration: 1.25ug/ml)
PCNA, Polyclonal Antibody (Cat# AAA834856)
Full Name
PCNA antibody
Gene Names
PCNA; ATLD2
Applications
IHC, WB
Pricing
$535/0.1 mL | $2,395/5x0.1 mL
SDS-PAGE
DNA replication licensing factor MCM2, Recombinant Protein (Cat# AAA954533)
Full Name
Recombinant Human DNA replication licensing factor MCM2 (MCM2)
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $395/0.1 mg (E-Coli) | $245/0.02 mg (E-Coli) | $6,205/5x1 mg (E-Coli)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z antibody at 1:500 dilution.)
Histone H2A.Z, Polyclonal Antibody (Cat# AAA837313)
Full Name
Histone H2A.Z antibody
Gene Names
H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
Applications
IF, ICC, IHC, WB
Purity
Histone H2A.Z antibody was purified by antigen-affinity chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
SDS-Page
(SDS-PAGE Analysis Purified ATRX Mouse Monoclonal Antibody (23c). Confirmation of Integrity and Purity of Antibody.)
ATRX/RAD54, Monoclonal Antibody (Cat# AAA4380175)
Full Name
ATRX/RAD54 (Alpha Thalassemia Mental Retardation)
Gene Names
ATRX; JMS; XH2; XNP; MRX52; RAD54; RAD54L; ZNF-HX
Reactivity
Human. Others not known.
Applications
EIA
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Ku (p70/p80), Monoclonal Antibody (Cat# AAA438044)
Full Name
Ku (p70/p80)
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human, Primates. Does not react with mouse, rat and chicken.
Applications
IF, IP, ICC, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with ATRX Rabbit Recombinant Monoclonal Antibody (ATRX/2900R).)
ATRX/RAD54 (Alpha Thalassemia Mental Retardation), Monoclonal Antibody (Cat# AAA4380661)
Full Name
ATRX/RAD54 (Alpha Thalassemia Mental Retardation)
Gene Names
ATRX; JMS; XH2; XNP; MRX52; RAD54; RAD54L; ZNF-HX
Reactivity
Human. Others not known.
Applications
Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Confirmation and in vitro characterization of Hit compounds. A) HeLa cells were treated with the ten compounds that produced the strongest reduction in pSer40/41MCM2 levels in the primary screen. Protein extracts were prepared and levels of residual pSer40/41MCM2 phosphorylation were measured by western blot analysis with the indicated antibodies. B) The same compounds were tested for their ability to inhibit CDC7 kinase activity in in vitro kinase assay. Kinase reactions were performed on a synthetic MCM2 substrate in the absence or presence of the indicated drug and reactions were run on SDS-PAGE gels. CDC7 activity on the synthetic MCM2 substrate was monitored by Western blotting with an anti-pSer108MCM2 antibody. As a control the levels of CDC7 kinase and synthetic MCM2 substrate present in each reaction were also assessed and shown to be similar in all reactions.From: FitzGerald J, Murillo LS, O'Brien G, O'Connell E, O'Connor A, et al. (2014) A High Through-Put Screen for Small Molecules Modulating MCM2 Phosphorylation Identifies Ryuvidine as an Inducer of the DNA Damage Response. PLoS ONE 9(6): e98891.)
MCM2, Monoclonal Antibody (Cat# AAA212357)
Full Name
MOUSE ANTI HUMAN MCM2
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Applications
WB
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (MCM7/1469).)
MCM7, Monoclonal Antibody (Cat# AAA4380106)
Full Name
MCM7 (Proliferation Marker)
Gene Names
MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Monoclonal Antibody (MCM7/1468).)
MCM7, Monoclonal Antibody (Cat# AAA4380105)
Full Name
MCM7 (Proliferation Marker)
Gene Names
MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot detection of RPA32/RPA2 in MCF7, Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s.)
RPA32/RPA2, Monoclonal Antibody (Cat# AAA475403)
Full Name
Anti-RPA32/RPA2 Mouse mAb
Gene Names
RPA2; REPA2; RPA32; RP-A p32; RP-A p34
Reactivity
Human
Applications
WB, IP, ICC
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CDT1 using LOVO whole cell lysates)
CDT1, Polyclonal Antibody (Cat# AAA9608602)
Full Name
CDT1 Antibody
Gene Names
CDT1; DUP; RIS2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot Analysis A) HeLa (B) Raji and C) HepG2 Cell Lysate Using MCM7 Monoclonal Antibody (MCM7/1466).)
MCM7, Monoclonal Antibody (Cat# AAA4380103)
Full Name
MCM7 (Proliferation Marker)
Gene Names
MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Immunohistochemistry (IHC)
(HC Suggested Anti-CDT1 antibodyTitration:2 ug/mlPositive Control:Mouse brain stem cells)
CDT1, Polyclonal Antibody (Cat# AAA3208168)
Full Name
CDT1 antibody - C-terminal region
Gene Names
CDT1; DUP; RIS2
Reactivity
Cow, Dog, Human, Mouse, Pig, Rabbit, Rat, Yeast
Applications
IHC, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Immunofluorescence (IF)
(MBS9600145 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
MCM5, Polyclonal Antibody (Cat# AAA9600145)
Full Name
MCM5 Antibody
Gene Names
MCM5; CDC46; MGORS8; P1-CDC46
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
