Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: MCM2 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 102 KDaObserved band size: 102 KDa)
Rabbit anti-Human DNA replication licensing factor MCM2 Polyclonal Antibody | anti-MCM2 antibody
Rabbit anti-human DNA replication licensing factor MCM2 polyclonal Antibody
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: MCM2 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 102 KDaObserved band size: 102 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Raji whole cell lysate, 293 whole cell lysateAll lanes: MCM2 antibody at 4.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 102 KDaObserved band size: 102 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7003843 diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7003843 diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(Immunofluorescence staining of MCF-7 cells with MBS7003843 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC)
(Immunofluorescence staining of MCF-7 cells with MBS7003843 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
Uniprot Description
MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.
Protein type: EC 3.6.4.12; DNA-binding
Chromosomal Location of Human Ortholog: 3q21
Cellular Component: chromatin; cytoplasm; MCM complex; microtubule cytoskeleton; nuclear chromosome, telomeric region; nuclear origin of replication recognition complex; nucleoplasm; nucleus
Molecular Function: ATP binding; DNA binding; DNA helicase activity; DNA replication origin binding; histone binding; metal ion binding; protein binding
Biological Process: cell cycle; DNA replication; DNA replication initiation; DNA strand elongation during DNA replication; DNA unwinding during replication; G1/S transition of mitotic cell cycle; mitotic cell cycle; nucleosome assembly
Research Articles on MCM2
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Product Notes
The MCM2 mcm2 (Catalog #AAA7003843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human DNA replication licensing factor MCM2 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA replication licensing factor MCM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the MCM2 mcm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA replication licensing factor MCM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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