Typical Testing Data/Standard Curve (for reference only)
Human REPIN1 ELISA Kit | REPIN1 elisa kit
Human REPIN1 (Replication Initiator 1) ELISA Kit
Gene Names
REPIN1; AP4; RIP60; ZNF464; Zfp464
Reactivity
Human
Synonyms
REPIN1; Human REPIN1 (Replication Initiator 1) ELISA Kit; REPIN1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human REPIN1. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human REPIN1 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
624
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for REPIN1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of REPIN1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REPIN1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human REPIN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REPIN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human REPIN1. You can calculate the concentration of Human REPIN1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REPIN1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human REPIN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REPIN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human REPIN1. You can calculate the concentration of Human REPIN1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,920 Da
NCBI Official Full Name
replication initiator 1 isoform 3
NCBI Official Synonym Full Names
replication initiator 1
NCBI Official Symbol
REPIN1
NCBI Official Synonym Symbols
AP4; RIP60; ZNF464; Zfp464
NCBI Protein Information
replication initiator 1; 60 kDa origin-specific DNA-binding protein; 60 kDa replication initiation region protein; ATT-binding protein; DHFR oribeta-binding protein RIP60; H_DJ0584D14.12; replication initiation region protein (60kD); zinc finger protein 464 (RIP60); zinc finger protein AP4
UniProt Protein Name
Replication initiator 1
Protein Family
UniProt Gene Name
REPIN1
UniProt Synonym Gene Names
RIP60; ZNF464
UniProt Entry Name
REPI1_HUMAN
Uniprot Description
REPIN1: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. Homodimers and homomultimers. Found in a complex with RIP60 and RIP100.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: nucleoplasm; nuclear membrane; lipid particle; nucleus; nuclear origin of replication recognition complex
Molecular Function: DNA binding; metal ion binding
Biological Process: DNA replication
Research Articles on REPIN1
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Product Notes
The Human REPIN1 repin1 (Catalog #AAA2510142) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510142 ELISA Kit recognizes Human REPIN1. It is sometimes possible for the material contained within the vial of "REPIN1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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