Western Blot (WB)
(Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500)
Rabbit MCM6 Polyclonal Antibody | anti-MCM6 antibody
MCM6 Polyclonal Antibody
Gene Names
mcm6; mis5; mmcm6
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
MCM6; Polyclonal Antibody; MCM6 Polyclonal Antibody; Mis5; P105MCM; MCG40308; anti-MCM6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
2mg/mL (varies by lot)
Sequence Length
821
Applicable Applications for anti-MCM6 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human MCM6
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of 293T, K562, NIH/3T3 and hela cell using MCM6 Polyclonal Antibody at dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer using MCM6 Polyclonal Antibody at dilution of 1:45)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human cervical cancer using MCM6 Polyclonal Antibody at dilution of 1:45)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human tonsil using MCM6 Polyclonal Antibody at dilution of 1:45)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human tonsil using MCM6 Polyclonal Antibody at dilution of 1:45)
Related Product Information for anti-MCM6 antibody
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,639 Da
NCBI Official Full Name
maternal DNA replication licensing factor mcm6
NCBI Official Synonym Full Names
minichromosome maintenance complex component 6<
NCBI Official Symbol
mcm6
NCBI Official Synonym Symbols
mis5; mmcm6
NCBI Protein Information
maternal DNA replication licensing factor mcm6; maternal Mcm6 protein; maternal minichromosome maintenance protein 6; mis5p; mmcm6; xMCM6
UniProt Protein Name
Maternal DNA replication licensing factor mcm6
Protein Family
UniProt Gene Name
mmcm6
UniProt Synonym Gene Names
mMCM6; xMCM6
UniProt Entry Name
MCM6M_XENLA
Uniprot Description
Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. The existence of maternal and zygotic forms of mcm3 and mcm6 suggests that specific forms of mcm2-7 complexes may be used during different stages of development.
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Product Notes
The MCM6 mmcm6 (Catalog #AAA2526839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCM6 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MCM6 mmcm6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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