Typical Testing Data/Standard Curve (for reference only)
Human RPA-70 ELISA Kit | RPA-70 elisa kit
Human RPA-70 (replication protein A) ELISA Kit
Gene Names
RpA-70; CG9633; D-RPA70; D-SSB; DmelCG9633; DmRPA; dmrpa1; dRP-A; DRP-A; i164; RP-A; RPA; RPA 70; RPA 70 kDa; Rpa-70; RpA1; RPA1; RpA70; RPA70; Ssb-70
Reactivity
Human
Synonyms
RPA-70; Human RPA-70 (replication protein A) ELISA Kit; RPA-70 elisa kit
Reactivity
Human
Specificity
This kit recognizes Human RPA1 in samples. No significant cross-reactivity or interference between Human RPA1 and analogues was observed.
Sequence Length
603
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.16-10ng/mL
Sensitivity
0.1ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RPA1 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RPA1 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for RPA-70 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human RPA1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RPA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RPA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RPA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RPA1. You can calculate the concentration of Human RPA1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RPA1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RPA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RPA1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RPA1. You can calculate the concentration of Human RPA1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,624 Da
NCBI Official Full Name
replication protein A 70
NCBI Official Synonym Full Names
Replication Protein A 70
NCBI Official Symbol
RpA-70
NCBI Official Synonym Symbols
CG9633; D-RPA70; D-SSB; DmelCG9633; DmRPA; dmrpa1; dRP-A; DRP-A; i164; RP-A; RPA; RPA 70; RPA 70 kDa; Rpa-70; RpA1; RPA1; RpA70; RPA70; Ssb-70
NCBI Protein Information
CG9633 gene product from transcript CG9633-RA; CG9633-PA; RpA-70-PA; drosophila replication protein A; replication protein A 70; single stranded-binding protein 70
UniProt Protein Name
Replication protein A 70 kDa DNA-binding subunit
UniProt Gene Name
RpA-70
UniProt Synonym Gene Names
RPA1; RP-A p70; RF-A protein 1; DmRPA1
UniProt Entry Name
RFA1_DROME
Uniprot Description
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
Research Articles on RPA-70
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Product Notes
The Human RPA-70 rpa-70 (Catalog #AAA2509013) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509013 ELISA Kit recognizes Human RPA-70. It is sometimes possible for the material contained within the vial of "RPA-70, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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