Testing Data
(Sample(30 ug whole cell lysate)A: H1299B: HeLa S37.5% SDS PAGEPrimary antibody diluted at 1: 1000)
Rabbit anti-Human RPA 70 kDa subunit Polyclonal Antibody | anti-RPA1 antibody
RPA 70 kDa subunit antibody
Immunohistochemistry: 1:100-1:250
Immunofluorescence: 1:100-1:200
Testing Data
(Sample(30 ug whole cell lysate)A: H1299B: HeLa S37.5% SDS PAGEPrimary antibody diluted at 1: 1000)
Testing Data
(Sample(30 ug whole cell lysate)A: H1299B: HeLa S37.5% SDS PAGEPrimary antibody diluted at 1: 1000)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit antibody at 1: 100 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit antibody at 1: 100 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit antibody at 1: 200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit antibody at 1: 200 dilution.)
NCBI and Uniprot Product Information
Uniprot Description
RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: nucleoplasm; male germ cell nucleus; lateral element; PML body; DNA replication factor A complex; nucleus
Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding
Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; homeostasis of number of cells within a tissue; base-excision repair; nucleotide-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; positive regulation of cell proliferation; nucleotide-excision repair, DNA damage removal; hemopoiesis; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA-dependent DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
Research Articles on RPA1
Similar Products
Product Notes
The RPA1 rpa1 (Catalog #AAA9401646) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RPA 70 kDa subunit antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPA 70 kDa subunit can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500-1:3000 Immunohistochemistry: 1:100-1:250 Immunofluorescence: 1:100-1:200. Researchers should empirically determine the suitability of the RPA1 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPA 70 kDa subunit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
