S100A1 recombinant protein

Recombinant Mouse S100A1 Protein (His tag)

Cat. Num. AAA2547069

• Gene Names: S100a1; S100; S100a; AI266795
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: S100A1; Recombinant Mouse S100A1 Protein (His tag); AI266795; S100; S100a; S100A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 94

• Application Notes: The recombinant mouse S100A1 consisting of 109 amino acids and has a calculated molecular mass of 12.5 kDa. rmS100A1 migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for S100A1 recombinant protein

Background: S100A1 is a Ca2+binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation.This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation.. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

Description: A DNA sequence encoding the mouse S100A1 (P56565) (Gly 2-Ser 94) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6755386
• NCBI GeneID: 20193
• NCBI Accession #: NP_035439.1
• NCBI GenBank Nucleotide #: NM_011309.3
• UniProt Accession #: P56565; O88949
• Molecular Weight: 10,505 Da
• NCBI Official Full Name: protein S100-A1
• NCBI Official Synonym Full Names: S100 calcium binding protein A1
• NCBI Official Symbol: S100a1
• NCBI Official Synonym Symbols: S100; S100a; AI266795
• NCBI Protein Information: protein S100-A1
• UniProt Protein Name: Protein S100-A1
• Protein Family: Protein
• UniProt Gene Name: S100a1

Uniprot Description

S100A1: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1. Interacts with FKBP4. Interacts with RYR1 and RYR2. Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. Belongs to the S-100 family.

Protein type: Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 3 F1|3 39.24 cM

Cellular Component: A band; cytoplasm; I band; M band; neuron projection; nucleus; sarcoplasmic reticulum; Z disc

Molecular Function: ATPase binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter