RSPO2 recombinant protein

RSPO2 Protein, Mouse, Recombinant (mFc Tag)

Cat. Num. AAA8123109

• Gene Names: Rspo2; ftls; AA673245; D430027K22; 2610028F08Rik
• Purity: >85% as determined by SDS-PAGE
• Synonyms: RSPO2; RSPO2 Protein; Mouse; Recombinant (mFc Tag); Mouse R-Spondin 2/FTLS/RSPO2 Protein (Fc Tag); 2610028F08Rik Protein; AA673245 Protein; D430027K22 Protein; ftls Protein; R-spondin 2; RSPO2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly205

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asn 24
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Rspo2 (NP_766403.1)(Met1-Gly205) was expressed with the Fc region of mouse IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RSPO2 recombinant protein

Background: R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt/beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2/Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2/Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.

References

Cadieu E, et al. (2009) Coat Variation in the Domestic Dog Is Governed by Variants in Three Genes. Science. 326: 150-153.Kazanskaya O, et al. (2004) R-Spondin2 is a secreted activator of Wnt/beta-catenin signaling and is required for Xenopus myogenesis. Dev Cell. 7(4): 525-34.Parker HG, et al. (2010) An insertion in the RSPO2 gene correlates with improper coat in the Portuguese water dog. J Hered. 101(5):612-7.

NCBI and Uniprot Product Information

• NCBI GI #: 27370216
• NCBI GeneID: 239405
• NCBI Accession #: NP_766403.1
• NCBI GenBank Nucleotide #: NM_172815.3
• UniProt Accession #: Q8BFU0; Q7TPX3
• Molecular Weight: 28,276 Da
• NCBI Official Full Name: R-spondin-2
• NCBI Official Synonym Full Names: R-spondin 2 homolog (Xenopus laevis)
• NCBI Official Symbol: Rspo2
• NCBI Official Synonym Symbols: ftls; AA673245; D430027K22; 2610028F08Rik
• NCBI Protein Information: R-spondin-2; cristin-2; mCristin-2; roof plate-specific spondin-2; cysteine-rich and single thrombospondin domain-containing protein 2
• UniProt Protein Name: R-spondin-2
• Protein Family: R-spondin
• UniProt Gene Name: Rspo2
• UniProt Synonym Gene Names: Cristin-2; mCristin-2
• UniProt Entry Name: RSPO2_MOUSE

Uniprot Description

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation

Cellular Component: extracellular space; cell surface; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding

Biological Process: osteoblast differentiation; embryonic forelimb morphogenesis; Wnt receptor signaling pathway; negative regulation of odontogenesis of dentine-containing teeth; response to stimulus; embryonic hindlimb morphogenesis; bone mineralization