FTLS / RSPO2 recombinant protein

Recombinant Human FTLS / RSPO2 Protein (186 Leu/Pro, Fc tag)

Cat. Num. AAA2545414

• Gene Names: RSPO2; CRISTIN2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: FTLS / RSPO2; Recombinant Human FTLS / RSPO2 Protein (186 Leu/Pro; Fc tag); UNQ9384; PRO34209; CRISTIN2; MGC35555; MGC43342; FTLS / RSPO2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 179

• Application Notes: The secreted recombinant human RSPO2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 425 amino acids and has a predicted molecular mass of 48.3 kDa. ?The apparent molecular mass of rh RSPO2/Fc monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for FTLS / RSPO2 recombinant protein

Background: R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype . When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.

Description: A DNA sequence encoding the human RSPO2 (Q6UXX9-1) N-teminal fragment (Met 1-Gly 205, 186 Leu/Pro) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 544583460
• NCBI GeneID: 340419
• NCBI Accession #: NP_001269792.1
• NCBI GenBank Nucleotide #: NM_001282863.1
• UniProt Accession #: Q6UXX9; Q4G0U4; Q8N6X6; B3KVP0
• Molecular Weight: 20,972 Da
• NCBI Official Full Name: R-spondin-2 isoform 2
• NCBI Official Synonym Full Names: R-spondin 2
• NCBI Official Symbol: RSPO2
• NCBI Official Synonym Symbols: CRISTIN2
• NCBI Protein Information: R-spondin-2
• UniProt Protein Name: R-spondin-2
• UniProt Gene Name: RSPO2
• UniProt Synonym Gene Names: hRspo2

NCBI Description

This gene encodes a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular region

Molecular Function: protein binding; receptor binding