RSPO1 active protein

RSPO1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122679

• Gene Names: Rspo1; Rspondin; R-spondin
• Purity: >95% as determined by SDS-PAGE
• Synonyms: RSPO1; RSPO1 Protein; Mouse; Recombinant (His Tag); Mouse R-Spondin 1/RSPO1 Protein (His Tag); R-spondin Protein; Rspondin Protein; R-spondin 1; RSPO1 active protein

• Host: CHO Stable Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln265

• Species: Mouse
• Activity: Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9ug/ml in the presence of 5ng/mL recombinant mouse Wnt3a.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2)(Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 ug/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.)

Related Product Information for RSPO1 active protein

Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

References

Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62.Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.

NCBI and Uniprot Product Information

• NCBI GI #: 227452351
• NCBI GeneID: 192199
• NCBI Accession #: NP_619624.2
• NCBI GenBank Nucleotide #: NP_619624.2
• UniProt Accession #: Q9Z132
• NCBI Official Full Name: R-spondin-1
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: Rspo1
• NCBI Official Synonym Symbols: Rspondin; R-spondin
• NCBI Protein Information: R-spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: Rspo1
• UniProt Synonym Gene Names: Cristin-3; mCristin-3

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4|4 D2.2

Cellular Component: extracellular space; nucleus

Molecular Function: G-protein-coupled receptor binding; heparin binding; protein binding; receptor binding

Biological Process: male meiosis; positive regulation of protein amino acid phosphorylation; positive regulation of Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin