Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse R-spondin-2 (RSPO2) Standard Curve.)
Mouse R-Spondin-2 (RSPO2) ELISA Kit | RSPO2 elisa kit
Mouse R-Spondin-2 (RSPO2) ELISA Kit
Gene Names
Rspo2; ftls; AA673245; D430027K22; 2610028F08Rik
Reactivity
Mouse
Synonyms
R-Spondin-2 (RSPO2); Mouse R-Spondin-2 (RSPO2) ELISA Kit; RSPO2; CRISTIN2; MGC35555; MGC43342; R-spondin family; member 2; RSPO2 elisa kit
Reactivity
Mouse
Specificity
Mouse R-spondin-2 (RSPO2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RSPO2. No significant cross-reactivity or interference between Mouse RSPO2 and analogues was observed.
Sequence Length
243
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse R-spondin-2 (RSPO2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse R-spondin-2 (RSPO2) Standard Curve.)
Related Product Information for RSPO2 elisa kit
Description: This Mouse R-spondin-2 (RSPO2) ELISA Kit employs a two-site sandwich ELISA to quantitate RSPO2 in samples. An antibody specific for RSPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1) signaling. Xenopus and human RSPO2 enhanced mouse Wnt3a signaling in transfected human embryonic kidney cells. C-terminally truncated Xenopus Rspo2, but not the full-length protein, was secreted from cells and retained its activity. Mutation analysis showed that the TSP1 domain of Xenopus Rspo2 was dispensable for activity, but the furin domains were required. RT-PCR analysis showed that Xenopus embryos injected with Wnt8 or beta-catenin induced Rspo2 and Rspo3. Overexpression of Rspo2 in Xenopus activated the Wnt/beta-catenin pathway upstream of dishevelled (DVL1), interfered with Tgfb-type growth factors, and promoted myogenesis.
Background: R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1) signaling. Xenopus and human RSPO2 enhanced mouse Wnt3a signaling in transfected human embryonic kidney cells. C-terminally truncated Xenopus Rspo2, but not the full-length protein, was secreted from cells and retained its activity. Mutation analysis showed that the TSP1 domain of Xenopus Rspo2 was dispensable for activity, but the furin domains were required. RT-PCR analysis showed that Xenopus embryos injected with Wnt8 or beta-catenin induced Rspo2 and Rspo3. Overexpression of Rspo2 in Xenopus activated the Wnt/beta-catenin pathway upstream of dishevelled (DVL1), interfered with Tgfb-type growth factors, and promoted myogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
R-spondin-2 isoform 2
NCBI Official Synonym Full Names
R-spondin 2
NCBI Official Symbol
Rspo2
NCBI Official Synonym Symbols
ftls; AA673245; D430027K22; 2610028F08Rik
NCBI Protein Information
R-spondin-2
UniProt Protein Name
R-spondin-2
Protein Family
UniProt Gene Name
Rspo2
UniProt Synonym Gene Names
Cristin-2; mCristin-2
UniProt Entry Name
RSPO2_MOUSE
Uniprot Description
R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation
Cellular Component: extracellular space; cell surface; extracellular region
Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding
Biological Process: osteoblast differentiation; embryonic forelimb morphogenesis; Wnt receptor signaling pathway; negative regulation of odontogenesis of dentine-containing teeth; response to stimulus; embryonic hindlimb morphogenesis; bone mineralization
Research Articles on RSPO2
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Product Notes
The Mouse RSPO2 rspo2 (Catalog #AAA9717103) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717103 ELISA Kit recognizes Mouse RSPO2. It is sometimes possible for the material contained within the vial of "R-Spondin-2 (RSPO2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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