RSPO3 active protein

RSPO3 Protein, Human, Recombinant (aa 1-146, His Tag)

Cat. Num. AAA8120719

• Gene Names: RSPO3; PWTSR; THSD2; CRISTIN1
• Purity: >96% as determined by SDS-PAGE
• Synonyms: RSPO3; RSPO3 Protein; Human; Recombinant (aa 1-146; His Tag); Human RSPO3/R-spondin 3 Protein (aa 1-146; CRISTIN1 Protein; PWTSR Protein; THSD2 Protein; R-spondin 3; RSPO3 active protein

• Host: HEK293 Cells
• Purity/Purification: >96% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val146

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2ug/ml (100uL/well) can bind Human RNF43 hFc. The EC50 of Human RNF43 hFc is 3.0-12.0ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2 ug/ml (100 ul/well) can bind Human RNF43 hFc, the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL.)

Related Product Information for RSPO3 active protein

Background: R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.

References

Aoki M, et al. (2007) R-spondin3 is required for mouse placental development. Dev Biol. 301(1): 218-26.Kazanskaya O, et al. (2008) The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development. Development. 135(22): 3655-64.Kim KA, et al. (2008) R-Spondin family members regulate the Wnt pathway by a common mechanism. Mol Biol Cell. 19(6): 2588-96.Ohkawara B, et al. (2011) Rspo3 Binds Syndecan 4 and Induces Wnt/PCP Signaling via Clathrin-Mediated Endocytosis to Promote Morphogenesis. Dev Cell. 20(3): 303-14.

NCBI and Uniprot Product Information

• NCBI GI #: 18490982
• NCBI GeneID: 84870
• NCBI Accession #: NP_116173.2
• NCBI GenBank Nucleotide #: NM_032784.4
• UniProt Accession #: Q9BXY4; Q5VTV4; Q96K87; B2RC27
• Molecular Weight: 33,233 Da
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: RSPO3
• NCBI Official Synonym Symbols: PWTSR; THSD2; CRISTIN1
• NCBI Protein Information: R-spondin-3; R-spondin 3 homolog; protein with TSP type-1 repeat; roof plate-specific spondin-3; thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: RSPO3
• UniProt Synonym Gene Names: PWTSR; THSD2; hPWTSR; hRspo3
• UniProt Entry Name: RSPO3_HUMAN

NCBI Description

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

Uniprot Description

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; G-protein-coupled receptor binding; frizzled binding; receptor binding

Biological Process: Wnt receptor signaling pathway through beta-catenin