Western Blot (WB)
(Anti-GMFB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human GMF Beta/GMFB Polyclonal Antibody | anti-GMFB antibody
Anti-GMF Beta/GMFB Antibody, Rabbit Polyclonal
Gene Names
GMFB; GMF
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
GMF Beta/GMFB; Polyclonal Antibody; Anti-GMF Beta/GMFB Antibody; Rabbit Polyclonal; GMFB Antibody; Rabbit PAb; Antigen Affinity Purified; glia maturation factor; beta; Anti-GMF Antibody; anti-GMFB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human GMF Beta/GMFB
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-GMFB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
ELISA: 1:25,000-1:50,000
Immunogen
Recombinant Human GMFB Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human GMFB (rh GMFB). GMFB specific IgG was purified by Human GMFB affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-GMFB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-GMFB rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-GMFB antibody
GMFB is a nerve growth factor which belongs to the actin-binding proteins ADF family, GMF subfamily. GMFB is involved in nervous system development, angiogenesis and immune function. It is especially crucial for the nervous system. GMFB causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. It contains 1 ADF-H domain and is phosphorylated after phorbol ester stimulation. GMFB overexpression in astrocytes results in the increase of BDNF production. GMFB expression is increased by exercise, thus BDNF is important for exercise-induction of BDNF.
References
Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40. Zaheer S, et al. (2011) Augmented expression of glia maturation factor in Alzheimer's disease. Neuroscience. 194:227-33. Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,713 Da
NCBI Official Full Name
glia maturation factor beta
NCBI Official Synonym Full Names
glia maturation factor, beta
NCBI Official Symbol
GMFB
NCBI Official Synonym Symbols
GMF
NCBI Protein Information
glia maturation factor beta; GMF-beta
UniProt Protein Name
Glia maturation factor beta
Protein Family
UniProt Gene Name
GMFB
UniProt Synonym Gene Names
GMF-beta
UniProt Entry Name
GMFB_HUMAN
Uniprot Description
GMF-beta: This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. Belongs to the actin-binding proteins ADF family. GMF subfamily.
Protein type: Actin-binding; Inhibitor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q22.2
Cellular Component: intracellular
Molecular Function: signal transducer activity; growth factor activity; protein kinase inhibitor activity; enzyme activator activity; actin binding
Biological Process: positive regulation of catalytic activity; nervous system development; negative regulation of protein kinase activity; locomotory behavior; learning; signal transduction; protein amino acid phosphorylation
Research Articles on GMFB
Similar Products
Product Notes
The GMFB gmfb (Catalog #AAA8106872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GMF Beta/GMFB Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GMF Beta/GMFB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000. Researchers should empirically determine the suitability of the GMFB gmfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GMF Beta/GMFB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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