SDS-Page
(SDS-PAGE and Coomassie Stain: Representative gel from|this lot. Purity was assessed by SDS-PAGE and Coomassie blue|staining using 3ug of S0096-02A.)
SAPKK3, active Active Protein
SAPKK3, active, Recombinant, Human (Stress-activated Protein Kinase Kinase 3, SAPK Kinase 3, SAPKK-3, SKK3, Dual Specificity Mitogen-Activated Protein Kinase Kinase 6, MAP Kinase Kinase 6, MAP2K6, MAPKK 6, MAPKK6, MKK6, PRKMK6, MAPK/ERK Kinase 6, MEK 6, M
Purity
Highly PurifiedPurified using amylose resin beads and activated with MEK Kinase. MEK Kinase was removed using Ni-NTA chromatography. Mr ~81kD; purity 80% as determined by SDS-PAGE and coomassie blue staining.
Synonyms
SAPKK3; active; Recombinant; Human (Stress-activated Protein Kinase Kinase 3; SAPK Kinase 3; SAPKK-3; SKK3; Dual Specificity Mitogen-Activated Protein Kinase Kinase 6; MAP Kinase Kinase 6; MAP2K6; MAPKK 6; MAPKK6; MKK6; PRKMK6; MAPK/ERK Kinase 6; MEK 6; M; active active protein
Purity/Purification
Highly Purified
Purified using amylose resin beads and activated with MEK Kinase. MEK Kinase was removed using Ni-NTA chromatography. Mr ~81kD; purity 80% as determined by SDS-PAGE and coomassie blue staining.
Purified using amylose resin beads and activated with MEK Kinase. MEK Kinase was removed using Ni-NTA chromatography. Mr ~81kD; purity 80% as determined by SDS-PAGE and coomassie blue staining.
Form/Format
Supplied as a frozen solution in 50mM Tris-HCl, pH 7.5, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.15M NaCl, 0.03% Brij-35, 20% glycerol.
Specific Activity
33u/mg using myelin basic protein (MBP) as a final substrate. 1u SKK3/MEK6 = 1u p38a/SAPK2a generated per minute. 1u p38a/SAPK2a = 1nmol phosphate incorporated into MBP per minute.
Preparation and Storage
Stable for 6 months at -70 degree C from date of shipment.
SDS-Page
(SDS-PAGE and Coomassie Stain: Representative gel from|this lot. Purity was assessed by SDS-PAGE and Coomassie blue|staining using 3ug of S0096-02A.)
SDS-Page
(SDS-PAGE and Coomassie Stain: Representative gel from|this lot. Purity was assessed by SDS-PAGE and Coomassie blue|staining using 3ug of S0096-02A.)
Testing Data
(16–80ng of enzyme|phosphorylated, 2uM unactive SAPK2a.)
Testing Data
(16–80ng of enzyme|phosphorylated, 2uM unactive SAPK2a.)
Related Product Information for SAPKK3, active active protein
Mitogen-activated protein (MAP) kinases are activated by dual phosphorylation on threonine and tyrosine residues. The activation is carried out by the family of enzymes known as MAP Kinase Kinases (SKKs, MKKs or MEKs). There are at least four distinct SKKs/MKKs/MEKs identified in mammalian cell types: MKK1/MKK2 that activates Erk1/Erk2; MKK3 that activates Mpk2/p38 MAPK; MKK4 that activates p38 and Jun kinase; and MKK5 that activates ErkK5/BMK1. A newly described SKK/MKK has been termed SKK3/MKK6 or MEK6. SKK3/MKK6 is closely related to MKK3 and selectively phosphorylates p38. However, unlike the previously described SKKs/MKKs/MEKs, SKK3/MKK6 exists in a variety of alternatively spliced isoforms and unlike MKK4 is not regulated by rac or Cdc42.
Product Categories/Family for SAPKK3, active active protein
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Product Notes
The SAPKK3, active (Catalog #AAA636209) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SAPKK3, active, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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