Rabbit anti-Human DLAT Polyclonal Antibody | anti-dlaT antibody

DLAT, CT (DLAT, DLTA, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, 70kD mitochondrial autoantigen of primary biliary cirrhosis, Dihydrolipoamide acetyltransferase component of pyruvate dehydroge

Cat. Num. AAA6005422

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DLAT; Polyclonal Antibody; CT (DLAT; DLTA; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial; 70kD mitochondrial autoantigen of primary biliary cirrhosis; Dihydrolipoamide acetyltransferase component of pyruvate dehydroge; Anti -DLAT; anti-dlaT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-dlaT antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: DLAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 587-617 amino acids from the C-terminal region of human DLAT.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 (lane 1), HepG2 (lane 2), Jurkat (lane 3) cell line lysates (35ug/lane) using MBS6005422. This demonstrates that MBS6005422 detected the DLAT protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS6005422 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005422 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells (right histogram) using MBS6005422 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-dlaT antibody

DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.

Product Categories/Family for anti-dlaT antibody

Antibodies; Abs to Enzymes; Acetyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 15609352
• NCBI GeneID: 888777
• NCBI Accession #: NP_216731.1
• NCBI GenBank Nucleotide #: NC_000962.3
• NCBI Official Full Name: DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase
• NCBI Official Symbol: dlaT
• NCBI Protein Information: DlaT, dihydrolipoamide acyltransferase, E2 component of pyruvate dehydrogenase
• Protein Family: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex